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Proliferation culture medium in blueberry tissue culture

A technology of proliferation medium and tissue culture, applied in the field of proliferation medium, can solve the problems of low reproduction coefficient, long rooting time and high management cost, and achieve the effects of high proliferation efficiency, short time and easy preparation

Inactive Publication Date: 2011-08-31
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cutting propagation of lingonberry requires a long time for rooting, about 60 days, high management costs, and low reproduction coefficient. Therefore, tissue culture factory propagation is an effective way to ensure the supply of seedlings
At present, there are occasional foreign literatures on the tissue culture research data of lingonberry, but they are not systematic, and the high-efficiency breeding system is relatively lacking, and there is no effective proliferation medium suitable for industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In March, take lingonberry sharpblue (the current production variety) about 15 cm from the middle and upper part of the current year's branches as explants, rinse with tap water for 15 minutes, gently scrub with gauze to remove stains, rinse with tap water for 15 minutes, and cut the rinsed branches into Small sections of 4-5cm, with 2-4 buds left for each section, put them in an ultra-clean workbench for disinfection. Disinfection procedure: 75% ethanol for 30 seconds, 0.1% mercury liter for 15 minutes (add 2-3 drops of Tween 80) and shake from time to time, then rinse with sterile water 4 times, 3 minutes each time. Cut off 0.5cm at both ends of the sterilized branches, then cut the branches into small sections of 1-2cm, keep 1-2 buds in each section, and finally inoculate the cut stem sections with buds into the pre-prepared added 0.5mg.L -1 On ordinary MS medium of ZT, culture at 25°C under 1500-2500Lex light for 2 weeks, and the light time is 12 hours / day. After ...

Embodiment 2

[0028] In March, take the middle and upper part of the lingonberry No. 3 variety (the current production variety) as explants, rinse with tap water for 15 minutes, gently scrub with gauze to remove stains, and then rinse with tap water for 15 minutes. Cut the final branches into small sections of 4-5cm, leave 2-4 buds in each section, and put them in an ultra-clean workbench for disinfection. Disinfection procedure: 75% ethanol for 30 seconds, 0.1% mercury liter for 15 minutes (add Tween 802-3 drops) and shake from time to time, then rinse with sterile water 4 times, 3 minutes each time. Cut off 0.5cm at both ends of the sterilized branches, then cut the branches into small sections of 1-2cm, keep 1-2 buds in each section, and finally inoculate the cut stem sections with buds into the pre-prepared added 0.5mg.L -1 On ordinary MS medium of ZT, culture at 25°C under 1500-2500Lex light for 2 weeks, and the light time is 12 hours / day. After 2 weeks, the germinated shoots were cu...

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Abstract

The invention relates to a proliferation culture medium formula in blueberry tissue culture, which consists of the components with different concentrations as follows: macroelements, microelements, iron salts, organic compositions and the like. By using the culture medium, the proliferation efficiency can be as high as more than 15 times; and the proliferation culture time is as short as only 25-30 days, and proliferation seedlings can reach 3-4cm in height, thus fully satisfying the need for rooting culture. Simultaneously, the culture medium consists of the compositions which have common sources without compounds with special forms, and has easy preparation.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture, in particular to a proliferation medium for tissue culture in the process of cranberry factory seedling cultivation. Background technique: [0002] Bilberry is a new generation of fruit with the fastest development in the world in recent years. It has unique nutritional and health functions. It can be eaten fresh, processed into jam and wine, extracted from natural anthocyanins, and used as raw materials for medicines. At present, it has been proved that the biologically active substances in the lingonberry fruit have the functions of improving eyesight, eliminating eye fatigue, and enhancing vision; they have obvious therapeutic effects on various malignant tumors such as rectal cancer; , the role of preventing Alzheimer's disease; can reduce the cholesterol level in the body, regulate blood sugar concentration, prevent and treat cardiovascular disease and type II diabetes; can enh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李凌李政
Owner SOUTHWEST UNIV
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