41results about How to "Good for transplanting" patented technology

The invention provides a process for producing the oriental lily sterilized seed globule in the bottle, comprising: choosing explant, sterilizing, inducing culture and getting the sterilized stem tip, then breeding culture, subculture, and producing the sterilized seed globule in the bottle directly. The invention produces the sterilized seed globule in the bottle directly to avoid the problem of long period of ball forming and the accumulation of virus existing in the oriental lily, the seed globule can be planted in the soil when out of the bottle, and the survival rate is high. The breeding procedure of the sterilized seed globule is simplified by modifying and optimizing the whole producing procedure for the lily seed globule, and the breeding time of sterilizing the oriental lily is shortened greatly, the quality of seed globule is improved when the producing cost is decreased, which reinforces market competitive power of the domestic self-breeding seed globule and is suitable for the mass production of the oriental lily sterilized seed globule.

The invention discloses a method for inducing blueberries to root by using liquid culture media and permeable matrix, comprising the following steps: (1) preparing rooting culturing media; (2) transferring the healthy and strong blueberry seedlings which are subcultured for 40d and are 3-4 cm high into the rooting culturing media, culturing the blueberry seedlings for 7-10d in the dark and for 12h / d for about 20d under intermitted illumination condition until the blueberry seedlings have 3-7 root systems of 0.5-2 cm long; and (3) transplanting the test-tube blueberry seedlings which are cultured for 30d to root, are 3-6 cm high and have the root length of 0.5-2 cm into the media in which the proportion of turfy soil to moss is 1:1, and managing the blueberry seedlings. The rooting culturing media is prepared according to the following substeps: 1) preparing 0.8 cm*1.2 cm filtering paper balls or defatted cotton balls or 0.2 cm*0.6 cm vermiculites which function as the matrix of the rooting culturing media; 2) preparing rooting culturing solution which has the WPM of 1 / 2, the IBA of 1.0-5.0 mg.L-1, the edible white sugar of 2 percent and the pH value of 5.0-5.2; and 3) adding 6-10 filtering paper balls or defatted cotton balls or 20-30 vermiculites which are soaked by the rooting culturing solution and adding 3-5 ml rooting culturing solution to each culturing bottle and capping the culturing to sterilize according to the routine method. The invention has short rooting period and low production cost, can ensure that 100 percent of the blueberry seedlings can root and over 95 percent of the transplanted blueberry seedlings can survive and realize the rapid industrialized propagation of the blueberry seedlings on a large scale.

The invention relates to a method for improving tissue culture reproductive speed of Alpinia zerumbet, which relates to the tissue culture technology of Alpinia zerumbet, and comprises the following steps that: A. seed collection is proceeded after 7 to 8 continuous sunny days, clumped Alpinia zerumbet plants which are used as seed are dug out from the soil 2 to 3 days before the seed production, soil is removed, and the Alpinia zerumbet plants are hung upside down to be dried for 2 to 3 days at a cool well-ventilated indoor place; B. lateral buds on tubers or spouts are selected as explant materials; C. adventitious buds are inducted to produce; D. adventitious buds are bred and cultured; E. adventitious buds are rooted to culture; and F. seedlings are transplanted. The rapid formation of adventitious buds is promoted through high temperature and high-concentration hormone, so the induction efficiency is improved, and the culture time is shortened; the formation of variegated leaf solid buds can be realized by changing the culture medium formula, reducing the culture temperature and increasing the light density, whitening variation can be reduced, the rapid breeding rate and seedling formation rate can be effectively improved, the factory-oriented production progress of the tissue cultured seedling can be accelerated, and the production cost can be reduced.

The invention discloses a greenhouse psammophyte culture medium. Four different matrix layers, namely a base layer, an original soil layer, a nutritive layer and a covering layer, are sequentially paved from bottom to top, wherein the base layer is one or a mixture of more than one of quartz, feldspar, mica, kaolinite, limestone and granite, the thickness of the base layer is 30-80cm, and particle size is 1-5cm; the original soil layer is obtained by mixing chestnut soil, gray desert soil and desert sandy soil in volume ratio of (1-2):10:(1-3), and the thickness of the original soil layer is 20-60cm; the nutritive layer is a mixture of organic matters, inorganic minerals, inorganic fertilizer, microelement fertilizer and microbial fertilizer, and the thickness of the nutritive layer is 20-100cm; and the covering layer is formed by vermiculite and / or volcanic rock, and the thickness of the covering layer is 5-10cm. The greenhouse psammophyte culture medium is easy to produce and low in cost and can obviously improve the transplanting survival rate of greenhouse landscape psammophytes when applied to cultivation of the greenhouse landscape psammophytes.

The invention discloses a crop transplanter which comprises a mounting frame, a feeding seat, a blanking mechanism, a rack and a driving mechanism. The driving mechanism is fixed to the center of thetop of the rack, second electric push rods and supports are uniformly mounted at two ends of the driving mechanism respectively, two seedling frames are uniformly hinged to the upper portion between the two supports, and a control handle seat is mounted at one end of the rack. The blanking mechanism is fixed between the control handle seat and the supports, and a feeding seat is arranged above theblanking mechanism. By installing a second motor and material guiding discs, the four material guiding discs are convenient to rotate through the rotation of the second motor, when a groove in the first material guiding disc enters the corresponding gap in a seedling tray, the grooves of the remaining three material guiding discs enter the corresponding gaps in the seedling tray in sequence, cropseedlings in the seedling tray fall at intervals in sequence, the crop seedlings are convenient to automatically drop one by one, the manual operation is reduced, the cost is reduced, and the workingefficiency is improved.

The invention discloses a woody plant cutting propagation method and a device for cutting propagation of the woody plant. The device for cutting propagation of the woody plant is composed of a water tank and a pipe which is communicated with the water tank. The pipe is provided with many holes in which plugs with small holes are inserted. The woody plant cutting propagation method is mainly realized through the following steps of: a, preparing a cutting wood; B, shearing and processing the cutting wood; c, processing a seed bed; d, performing cutting; e, managing after cutting; and f, transplanting after rootage. According to the woody plant cutting propagation method, advance ring cutting is performed for realizing advance healing of an incision, preventing infection and improving nutrient substance accumulation on a branch. The cutting wood is inserted into the pipe through the rubber plug, and a relatively high water pressure in the pipe presses water into the cutting wood, thereby ensuring enough water supply to the cutting wood and furthermore improving a survival rate.

The invention relates to the field of agricultural equipment, in particular to an agricultural transplant device. The agricultural transplant device comprises a support frame, an adjustment mechanism,a seedling removing mechanism and a transplanting mechanism; the adjustment mechanism is mounted on one side of the support frame, the transplanting mechanism is fixedly connected with the adjustmentmechanism, the seedling removing mechanism is fixedly connected with the support frame and located above the transplanting mechanism, and the transplanting mechanism comprises a lifting component, adriving component, a transmission component and a transplanting component, the lifting component is fixedly connected with the adjusting mechanism, the transplanting component is fixedly connected with the lifting component, the driving component is mounted on one side of the transplanting component, and the transplanting component and the driving component are in transmission connection through the transmission component. The agricultural transplant device has the advantages that the device does not need to be driven by pedals, and can automatically return to facilitate seedling feeding, so that the labor intensity and the amount of labor of farmers can be further reduced, and the seedling transplanting efficiency can be improved.

The invention relates to a method for transplanting lagerstroemia plant trunk-cultivation modeling seedlings and belongs to the technical field of plant cultivation. The method comprises the following steps that 1, pits are formed in base soil and 2, mud is prepared with the match of manual operation. The method solves the problem that when inarching transplantation is conducted on a certain number of lagerstroemia plant seedlings in a traditional planting method, root gaps can not be filled and covered with solid soil. Root systems of the seedlings are completed filled and covered according to the mud filling method, transplanting survival is facilitated, and smooth conduction of inarching truck-cultivation work is guaranteed. Meanwhile, organic matter is added, so that soil fertility is increased, root growth of the seedlings is facilitated, and the added pesticide is beneficial to prevention and treatment of soil insects and soil-borne diseases.

The invention discloses an agricultural planting box with a drilling function. The agricultural planting box comprises a water storage mechanism and a planting mechanism. The water storage mechanism comprises a water storage tank, an observation window, a planting box and a funnel. The planting box is arranged in the water storage tank in a sleeving mode. One side of the upper portion of the waterstorage tank is connected with the funnel through a water inlet pipe. The planting mechanism comprises a bottom cover, a top cover and a hole retaining column, wherein the top cover covers the upperpart of the planting box, and the bottom cover is mounted on the lower part of the planting box through hinges. By injecting water into the water storage tank, the arranged funnel, the water inlet pipe and a partition plate can effectively prevent the water in the water storage tank from spilling out in the transportation process, so that the normal water consumption requirement of the crops is guaranteed in the transportation process; when soil is filled into the planting box, the hole retaining column can retain the planting holes needed for planting, so that when crops are transplanted, theholes do not need to be drilled again, and the crop transplanting work is facilitated.

The invention discloses a special culture medium for perilla frutescens, which comprises the following components: 1 / 4-1 / 2 of an MS culture medium, 0.2-0.4mg / L of naphthylacetic acid, 0.7-1mg / L of brassinolide, 0.8-1.2mg / L of triacontanol, 0.02-0.05mg / L of daminozide, 3-5g / L of white granulated sugar and 8-10g / L of agar. The brassinolide, the triacontanol and the daminozide are added into the traditional plant culture medium for the perilla frutescens, the triacontanol and the naphthylacetic acid which are synergistic can effectively promote the growth of roots, stems and leaves of the perilla frutescens, and a small quantity of daminozide can coordinate effects of the components and regulate the nutrition distribution in the perilla frutescens culture process, so that the perilla frutescens roots grow stronger, and the perilla frutescens is transplanted and survives easily; moreover, the brassinolide, the triacontanol and the daminozide have different degrees of disease resistance, and can inhibit the growth of germs, thereby improving the survival rate of the perilla frutescens and remarkably reducing the browning rate of the perilla frutescens.

The invention discloses a hormone-free corn callus regeneration method. The hormone-free corn callus regeneration method comprises the following steps: culturing granular corn calluses with good growth vigor on a hormone-free embryoid induction culture medium at 24-26 DEG C for 20-23 days, and inducing to generate an embryoid; transferring the embryoid to a hormone-free regeneration culture medium, wherein the culturing temperature is 24-26 DEG C, the illumination intensity is 12000lux-18000lux and the illumination time each day is 16h; culturing until roots and buds are generated to obtain a regenerated plant; and then continually culturing the regenerated plant for 6-7 days to raise strong seedlings under the following conditions that the temperature is 26-28 DEG C, the illumination intensity is 24000lux-30000lux and the illumination time each day is 16h. According to the hormone-free corn callus regeneration method, the osmotic pressure of a regeneration culture phase is regulated and controlled by changing the use amount of sucrose so as to realize the efficient regeneration of the calluses, and the whole process does not need plant hormones. The method not only saves the experiment cost, but also avoids poisoning tissue materials due to application of hormones for a long time.

The invention provides a festuca arundinacea tissue culture and differentiation regeneration method. The method comprises the following steps of S1, disinfecting and sterilizing seeds; S2, carrying out induction culture on embryonic calluses of explants; S3, carrying out proliferation culture on the calluses; S4, performing differentiation culture of the embryogenic calluses and rapid proliferation of immature embryos; and S5, performing strong seedling rooting culture and aseptic seedling transplanting. The method is simple to operate, the embryogenic calluses are easily formed by seed germs, complete plants with rhizomes and leaves are formed on a differential medium, and formed tissue culture seedlings are high in transplanting survival rate. Through embryoid multiplication culture, a large number of embryoid cluster buds or plantlets can be formed in a short period of 2 months. The cluster buds or plantlets only need to be cultured in a basic culture medium for about 20 days to form complete robust seedlings with roots, stems and leaves.

The invention discloses a method for constructing a malus asiatica cotyledon regeneration system. The method comprises the following steps of I, performing pretreatment; II, performing sterilization;III, inducing adventitious buds; IV, performing extending for strengthening buds and performing culturing; V, performing rooting culturing; and IV, performing transplanting. Malus asiatica cotyledon is used as an explant, in vitro regeneration is completed, and the technical solution provided by the invention is used for in vitro regeneration of malus asiatica, so that the induction sprouting rateand the value increment coefficient are increased. Experiment prove that obtained regrowth seedlings have the advantages that buds are strong, main roots are healthy, and seedling transplanting survival rate is high. The method for constructing a malus asiatica cotyledon regeneration system provided by the invention provides a new technique and method for malus asiatica heredity transformation and molecular breeding.

The invention provides a root induction method for Chinese fir test-tube plantlets. The method specifically includes the steps that the robust Chinese fir test-tube plantlets are inoculated to high-concentration auxin to be stimulated and induced to root and then are inoculated to an NAA auxin solution, the test-tube plantlets obtained after stimulation treatment are inoculated into a blank culture medium to be cultured for 1 day, the Chinese fir test-tube plantlets are treated in the NAA auxin solution and then are cultured at the constant temperature of 25 DEG C under LED red light, and root induction culture of the Chinese fir test-tube plantlets can be completed; the Chinese fir test-tube plantlets obtained after root induction culture are transplanted into a greenhouse and are hardened at the room temperature; the base parts of the Chinese fir tissue-cultured plantlets are treated with ABT1+IAA+yellow mud after the culture medium is cleaned away, then the hardened Chinese fir plantlets are transplanted to a light-medium culture cup, and rooting treatment of the Chinese fir test-tube plantlets is completed. According to the method, after stimulation treatment is carried out with high-concentration auxin ANN, root source bases can be easily formed; then, the Chinese fir test-tube plantlets are transferred into the blank culture medium, so that elongation growth of the root source bases is promoted; the rooting percentage is increased to 95.8% from 10%.

The invention discloses a rolling type small flowerpot soil spreading device used for a garden for simulating of a running robot. The rolling type small flowerpot soil spreading device comprises a box body, wherein the bottom parts of two sides of the box body are independently provided with supporting frames; the bottom of each supporting frame is provided with a supporting foot; the top of the box body is provided with a material feeding hopper; the bottom of the box body is provided with a material outlet; a material discharging part is arranged below the material outlet; a rolling device is arranged in the box body; the lower part between the supporting frames on two ends is provided with a fixing frame; in addition, two sides of the fixing frame are independently fixedly connected with the lower parts of the corresponding side edges of the supporting frames through a horizontal plate; and a flowerpot body is arranged in the fixing frame. The rolling type small flowerpot soil spreading device has the beneficial effects that the rolling device capable of simulating the structural form of the running robot is adopted to roll soil to avoid a phenomenon that caking soil directly falls into the flowerpot to affect subsequent transplantation work, meanwhile, working labor force of a manual operation is reduced, and working efficiency is improved.

The invention relates to the field of plant tissue culture, in particular to a tissue culture method for promoting strong roots of potato seedlings. The tissue culture method comprises the following steps: (1) carrying out material collection and disinfection; (2) stripping stem tips: stripping outer layer leaves of the sterilized potato apical buds under a binocular dissection mirror, and leavingonly 1-2 leaf primordia; (3) carrying out primary culture: inoculating 5-6 stem tips with sizes of 0.1-0.2mm in a primary culture medium for culturing; (4) carrying out rooting culture: inoculating the sterile seedlings, obtained in step (3), in a rooting culture medium in a form of single plants for culturing so as to obtain virus-free test-plantlets. The tissue culture method provided by the invention utilizes stem tip in vitro culture to solve the problem of large consumption of seed potatoes; furthermore, the primary culture medium, the rooting culture medium and culture conditions are optimized, so that the method not only can enable potatoes to have more roots and rapidly take roots, but also can enable the tissue cultured seedlings of the potatoes to be healthy and strong so as toenable the seedlings to be more easily transplanted and more easily survive.

The invention relates to the technical field of plant cultivation, in particular to a cunninghamia lanceolata tissue culture seedling transplanting matrix and a preparation method thereof. The transplanting matrix comprises the following components in parts by volume: 1-3 parts of fermented bark, 2-3 parts of fermented wood bran, 4-5 parts of foamed coconut bran and 0.5-1 part of yellow soil. According to the invention, by taking the bark, the wood bran and the coconut bran as main materials, and the yellow soil as an auxiliary material, the cunninghamia lanceolata tissue culture seedling transplanting matrix is prepared, so that the content of the organic matters in the bark is high, the antibacterial property and the stress resistance are achieved, and the content of organic matters in the bark is high, including cellulose, hemicellulose, lignin, or other microelement; the carbon-nitrogen ratio of the wood bran is high, and the air permeability and the water-retaining property are good, the coconut bran is loose in texture, natural decomposition is slow, and the service life of the matrix can be prolonged, the texture of the yellow soil is compact, the loss of moisture and nutrients can be effectively prevented, and the prepared matrix is very suitable for transplanting cunninghamia lanceolata tissue culture seedlings.

The invention provides a cutting seedling raising method for Armeniaca sibirica, and relates to the technical field of vegetative propagation of plants. The cutting seedling raising method for the Armeniaca sibirica comprises the steps of cutting slip selection, cutting slip treatment, cutting, rooting management and transplanting seedling raising, wherein cutting slips are soaked in IBA with the concentration of 400-800 mg / L and salicylic acid with the concentration of 200-600 mg / L, turf, mountain base soil and river sand are mixed according to the volume ratio of (1-3): (3-5): (1-3) to serve as a cutting medium, the rooting rate of the Armeniaca sibirica cutting slips and the growth traits of cutting seedlings can be obviously improved, and the problem that the Armeniaca sibirica cutting is difficult to root is solved.

The invention discloses a method for plug-tray greenwood cutting seedling culture of rhododendron ovatum. A semi-lignified twig grown in a current year is clipped and taken as a cutting, and a cuttingshoot is clipped from the cutting and soaked in an ABT reagent of concentration of 50-100 mg / L for 4-6 h; after soaking, the cutting shoot is inserted into a seedling culture plug tray for cutting, thorough watering is conducted immediately after cutting, a sterilizing agent is sprayed, and the seedling culture plug tray is coated with a thin film and shielded; and then, watering is conducted incase of dryness, and transplantation can be conducted when an axillary bud of the cutting shoot germinates and a new root of the cutting shoot grows to 1-3 cm. In this way, the method can greatly increase a cutting rooting rate of rhododendron ovatum, can increase a cutting survival rate of rhododendron ovatum to about 73.33%, can save a matrix and space of seedling culture, does not need establishment of a dedicated arc shed, realizes good moisture preservation effects, does not need frequent water supplementation, is simple and easy in operations, low in production cost and high in economicbenefits, can promote transplantation survival and has a high application value.

The invention relates to a method for improving tissue culture reproductive speed of Alpinia zerumbet, which relates to the tissue culture technology of Alpinia zerumbet, and comprises the following steps that: A. seed collection is proceeded after 7 to 8 continuous sunny days, clumped Alpinia zerumbet plants which are used as seed are dug out from the soil 2 to 3 days before the seed production, soil is removed, and the Alpinia zerumbet plants are hung upside down to be dried for 2 to 3 days at a cool well-ventilated indoor place; B. lateral buds on tubers or spouts are selected as explant materials; C. adventitious buds are inducted to produce; D. adventitious buds are bred and cultured; E. adventitious buds are rooted to culture; and F. seedlings are transplanted. The rapid formation of adventitious buds is promoted through high temperature and high-concentration hormone, so the induction efficiency is improved, and the culture time is shortened; the formation of variegated leaf solid buds can be realized by changing the culture medium formula, reducing the culture temperature and increasing the light density, whitening variation can be reduced, the rapid breeding rate and seedling formation rate can be effectively improved, the factory-oriented production progress of the tissue cultured seedling can be accelerated, and the production cost can be reduced.

The invention relates to a Xinjiang Hotan rose rhizome seedling raising and large-scale cultivation method. The method comprises the steps of seedling raising material selection, nutrient soil adaptation, root burying seedling raising, transplanting and field management. By means of the method, standard Hotan rose planting operation regulations are established, and rapid artificial seedling raising, field cultivation and standard planting are provided, and moreover, the propagation period of the Hotan roses is shortened, the method is suitable for industrial cultivation of the Hotan roses, anda foundation is laid for rapid propagation of the Hotan roses. Technical support is provided for forming a Hotan rose industry chain, so that the special species generate huge economic benefits; anda scientific basis is provided for excellent variety breeding and rapid propagation research of the Hotan roses. The method solves the key problems of quality real-time evaluation standard, high addedvalue product development, comprehensive resource utilization and the like suitable for Hotan rose variety breeding, large-scale artificial planting, seedling culture and planting processes in the southern Xinjiang region.

The invention provides a root induction method for Chinese fir test-tube plantlets. The method specifically includes the steps that the robust Chinese fir test-tube plantlets are inoculated to high-concentration auxin to be stimulated and induced to root and then are inoculated to an NAA auxin solution, the test-tube plantlets obtained after stimulation treatment are inoculated into a blank culture medium to be cultured for 1 day, the Chinese fir test-tube plantlets are treated in the NAA auxin solution and then are cultured at the constant temperature of 25 DEG C under LED red light, and root induction culture of the Chinese fir test-tube plantlets can be completed; the Chinese fir test-tube plantlets obtained after root induction culture are transplanted into a greenhouse and are hardened at the room temperature; the base parts of the Chinese fir tissue-cultured plantlets are treated with ABT1+IAA+yellow mud after the culture medium is cleaned away, then the hardened Chinese fir plantlets are transplanted to a light-medium culture cup, and rooting treatment of the Chinese fir test-tube plantlets is completed. According to the method, after stimulation treatment is carried out with high-concentration auxin ANN, root source bases can be easily formed; then, the Chinese fir test-tube plantlets are transferred into the blank culture medium, so that elongation growth of the root source bases is promoted; the rooting percentage is increased to 95.8% from 10%.

The scheme discloses a Sichuan pepper cultivation method, which comprises the following steps of: S1: seed processing: putting a seed in a container, adding boiled water (of which the temperature is 94-98 DEG C) of which the amount is 2-3 times of volume of the seed, stirring for 4-5 min, then, adding cold water until the temperature of water is 25-30 DEG C, then, dipping for 4-5h, taking out theseed, then, continuously dipping the seed in clean cold water for 28-36h, taking out the seed from the water, and enabling the seed to stand and keep the temperature for 4-5 days in a temperature environment of 30-35 DEG C; S2: soil preparation and sowing: after soil preparation and furrow formation, adopting a sowing way for sowing seeds; S3: seedling cultivation; S4: transplantation; and S5: trimming and shaping. The growth speed of the Sichuan pepper cultivated through the scheme disclosed by the invention is improved, the incidence rate of a Sichuan pepper tree is lowered, and the survivalrate of the Sichuan pepper is improved.

The invention relates to the technical field of plant tissue culture, and specifically discloses a method for constructing a peanut cotyledon regeneration system, comprising the following steps: (1) pretreatment; (2) sterilization; (3) callus culture; (4) Cluster bud induction; (5) Proliferation culture; (6) Strong seedling culture; (7) Rooting culture; (8) Transplanting. The method for constructing a stable peanut cotyledon regeneration system provided by the invention provides a new technology and method for peanut genetic transformation and molecular breeding.