A kind of method of tall fescue tissue culture and differentiation regeneration
A technique of tissue culture and tall fescue, applied in the field of genetic engineering, can solve the problems of low reproductive coefficient, slow growth, and germplasm decline of ramets, and achieve the effects of easy transplanting and survival, simple operation, and solving difficult reproduction
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[0018] It is easy to understand that, according to the technical solutions of the present invention, without changing the essential spirit of the present invention, those of ordinary skill in the art can propose various alternative structures and implementations. Therefore, the following specific embodiments are only exemplary descriptions of the technical solutions of the present invention, and should not be regarded as the whole of the present invention or as limitations or restrictions on the technical solutions of the present invention.
[0019] A method for tissue culture and differentiation and regeneration of tall fescue, comprising the following steps:
[0020] S1. Disinfection and sterilization of seeds: 50% sulfuric acid oscillating and soaking for 25 minutes, rinse with sterile water to remove impurities such as separated glumes and papules, then disinfect with 70% alcohol for 45s, rinse with sterile water for 3 times, and finally use 30 % sodium hypochlorite (avail...
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