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A kind of method of tall fescue tissue culture and differentiation regeneration

A technique of tissue culture and tall fescue, applied in the field of genetic engineering, can solve the problems of low reproductive coefficient, slow growth, and germplasm decline of ramets, and achieve the effects of easy transplanting and survival, simple operation, and solving difficult reproduction

Active Publication Date: 2022-06-24
南京栢特隆生物科技有限公司
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Problems solved by technology

[0003] It is very difficult to propagate tall fescue through seeds, so it is often used in production to propagate by division. However, division propagation not only has a low reproduction coefficient, but also slow growth, and it is easy to cause serious decline in germplasm, which affects the quality of tall fescue. Rapid Propagation and Application of Germplasm

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  • A kind of method of tall fescue tissue culture and differentiation regeneration
  • A kind of method of tall fescue tissue culture and differentiation regeneration
  • A kind of method of tall fescue tissue culture and differentiation regeneration

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Embodiment Construction

[0018] It is easy to understand that, according to the technical solutions of the present invention, without changing the essential spirit of the present invention, those of ordinary skill in the art can propose various alternative structures and implementations. Therefore, the following specific embodiments are only exemplary descriptions of the technical solutions of the present invention, and should not be regarded as the whole of the present invention or as limitations or restrictions on the technical solutions of the present invention.

[0019] A method for tissue culture and differentiation and regeneration of tall fescue, comprising the following steps:

[0020] S1. Disinfection and sterilization of seeds: 50% sulfuric acid oscillating and soaking for 25 minutes, rinse with sterile water to remove impurities such as separated glumes and papules, then disinfect with 70% alcohol for 45s, rinse with sterile water for 3 times, and finally use 30 % sodium hypochlorite (avail...

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Abstract

The present invention proposes a method for tall fescue tissue culture and differentiation regeneration, comprising the following steps: S1, disinfection and sterilization of seeds; S2, induction and culture of explant embryogenic callus; S3, callus proliferation and culture ; S4, differentiation culture of embryogenic callus and rapid proliferation of immature embryos; S5, rooting culture of strong seedlings and transplanting of sterile seedlings. The invention has simple operation, and the seed germ can easily form embryogenic callus, and a complete plant with roots, stems and leaves can be formed on the differentiation medium, and the transplanted survival rate of the formed tissue culture seedlings is high. After embryoid body proliferation and culture, a large number of embryoid body cluster buds or plantlets can be formed within a short period of 2 months. Cluster buds or plantlets only need about 20 days of cultivation in minimal medium to form complete robust seedlings with roots, stems and leaves.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for tissue culture and differentiation and regeneration of tall fescue. Background technique [0002] Tall fescue (Festuca elata Keng ex E. Alexeev) is a perennial herb of the subgenus Fescue of the Poaceae family. It is a perennial herb because of its strong adaptability, outstanding stress resistance, strong resistance to trampling and disease, and no dormancy in summer. Grass species suitable for widespread promotion and use are indispensable landscaping lawn ground cover plants in cool-season landscaping. [0003] It is very difficult to propagate tall fescue through seeds, so ramet propagation is often used in production. However, ramet propagation not only has a very low reproduction coefficient, but also slows growth. Rapid propagation and popularization of germplasm. SUMMARY OF THE INVENTION [0004] The technical problem to be solved by the present inventi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许磊
Owner 南京栢特隆生物科技有限公司
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