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Method for improving tissue culture reproductive speed of Alpinia zerumbet

A technique of Mosaic Ginger and tissue culture, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problem that the price of tissue culture seedlings and sieved seedlings remains high, and affects the factory production of tissue culture seedlings and tissue culture seedlings. In short supply and other problems, to achieve the effect of facilitating transplanting survival, speeding up the process of factory production, and reducing the phenomenon of albino variation

Inactive Publication Date: 2013-07-31
东莞市生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the report about the tissue culture of Alpinia mosaic is seldom at present, and because of the reason of Alpinia mosaic plant itself, in actual production, there are the problems of low proliferation rate, serious separation of chimeras, and poor seedling rate in the tissue culture propagation of Alpinia mosaic. It has greatly affected the process of industrial production of tissue culture seedlings, the supply of tissue culture seedlings is in short supply, and the prices of tissue culture seedlings and screened seedlings have remained high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A. Seed collection should be carried out after 7 to 8 consecutive sunny days. 2 to 3 days before seed production, dig out the Alpinia japonica plants that need to be planted in clumps from the soil, remove the soil, and hang them upside down in a ventilated and cool place indoors. Dry for 2 to 3 days.

[0037] B. Select the pumped shoots on the tubers as explant materials.

[0038] C. Induction of adventitious buds:

[0039] (1) Disinfection method: After removing the overlong leaves and root system on the tuber, rinse the soil under tap water, and cut off the tender shoots and old stems used as explant materials, and dip them in wet degreasing cotton 0.5% washing powder water lightly wipes the surface of shoots and tubers used as explant materials, and then rinses under tap water for 10-20 minutes. On a clean workbench, remove excess leaves and outer bracts on the tender buds used as explant materials; keep the size of each piece of explant material at a size of 1-2c...

Embodiment 2

[0059] A. Seed collection should be carried out after 7 to 8 consecutive sunny days. 2 to 3 days before seed production, dig out the Alpinia japonica plants that need to be planted in clumps from the soil, remove the soil, and hang them upside down in a ventilated and cool place indoors. Dry for 2-3 days;

[0060] B. select the tender buds that have taken out on the tuber as explant material;

[0061] C. Induction of adventitious buds:

[0062] (1) Disinfection method: After removing the overlong leaves and root system on the tuber, rinse the soil under tap water, and cut off the tender shoots and old stems used as explant materials, and dip them in wet degreasing cotton 0.5% washing powder water lightly wipes the surface of shoots and tubers used as explant materials, and then rinses under tap water for 10-20 minutes. On a clean workbench, remove excess leaves and outer bracts on the tender buds used as explant materials; keep the size of each piece of explant material at a...

Embodiment 3

[0082] A. Seed collection should be carried out after 7 to 8 consecutive sunny days. 2 to 3 days before seed production, dig out the Alpinia japonica plants that need to be planted in clumps from the soil, remove the soil, and hang them upside down in a ventilated and cool place indoors. Dry for 2-3 days;

[0083] B. select the lateral bud on the tuber as the explant material;

[0084] C. Induction of adventitious buds:

[0085] (1) Disinfection method: after removing the overlong blade and root system on the tuber, rinse the soil under tap water, and dip in 0.5% washing powder water by weight with wet degreasing cotton and gently wipe the tuber used as explant material Surface, then rinse under running water for 10-20 minutes. On a clean workbench, cut off the large lateral buds used as explant materials on the tuber, and leave the small lateral buds on the tuber, keeping the size of each explant material at 1-2cm×1-2cm . According to two different types of large lateral ...

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Abstract

The invention relates to a method for improving tissue culture reproductive speed of Alpinia zerumbet, which relates to the tissue culture technology of Alpinia zerumbet, and comprises the following steps that: A. seed collection is proceeded after 7 to 8 continuous sunny days, clumped Alpinia zerumbet plants which are used as seed are dug out from the soil 2 to 3 days before the seed production, soil is removed, and the Alpinia zerumbet plants are hung upside down to be dried for 2 to 3 days at a cool well-ventilated indoor place; B. lateral buds on tubers or spouts are selected as explant materials; C. adventitious buds are inducted to produce; D. adventitious buds are bred and cultured; E. adventitious buds are rooted to culture; and F. seedlings are transplanted. The rapid formation of adventitious buds is promoted through high temperature and high-concentration hormone, so the induction efficiency is improved, and the culture time is shortened; the formation of variegated leaf solid buds can be realized by changing the culture medium formula, reducing the culture temperature and increasing the light density, whitening variation can be reduced, the rapid breeding rate and seedling formation rate can be effectively improved, the factory-oriented production progress of the tissue cultured seedling can be accelerated, and the production cost can be reduced.

Description

technical field [0001] The invention relates to the tissue culture propagation technology of Alpinia japonica, in particular to a method for improving the tissue culture propagation speed of Alpinia japonica. technical background [0002] Variegata (Alpinia zerumbet cv. Variegata), also known as Alpinia zerumbet cv. Variegata (Taiwan), is a variant of Alpinia genus Ginger in the family Zingiberaceae. It is a perennial herbaceous foliage plant. The plant height is 0.6-2m, clustered, leathery leaves, dark green with golden vertical stripes, it is a foliage and flower viewing plant with high ornamental value, and it is also an indispensable species in various garden landscape designs. [0003] Although Alpinia mosaic can bear fruit in the natural state, the golden leaf spot traits of the progeny plants after the seeds are sown cannot be inherited, so they can only reproduce by natural branching, but the coefficient is very low, and the growth period is long, so it lags behind. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 陈春满张善信陈青云
Owner 东莞市生物技术研究所
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