A kind of method of banana MS-free tissue culture

Abstract

The invention provides a method for tissue culture of bananas without MS. In the banana cluster bud proliferation stage, the subculture stage and the rooting culture stage, the cluster bud proliferation tissue culture fluid, the subculture tissue culture fluid and the root tissue culture fluid are respectively used. The cassava ethanol anaerobic fermentation broth medium is included in the cluster bud proliferation tissue culture fluid, the subculture tissue culture fluid and the rooting tissue culture fluid. Adopting the technical scheme of the present invention, using the cassava alcohol anaerobic fermentation liquid to replace the basic medium MS, not only does not have any influence on the normal growth of the three different stages of banana tissue culture, but also greatly reduces the production cost, turning waste into wealth, and improving ecological quality. The environment is more environmentally friendly, and the cultivated banana seedlings can also achieve the effect of the seedlings cultivated by MS tissue culture in the past, and the number and sturdiness of the roots are more obvious. The greening period is earlier than the basic MS medium, and it is easier to transplant the tissue culture seedlings Survival provides favorable conditions for large-scale tissue culture production.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to a method for tissue culture of bananas without MS. Background technique [0002] Banana (Musa paradisiaca L.) belongs to Musa family (Musaceae), Musa genus (Musa L), is a perennial large evergreen herbaceous monocot plant, originated in tropical Asia, and is one of the main fresh fruit species in the world. my country has been cultivating bananas for more than two thousand years, and is one of the countries with the longest history of cultivating bananas. South China is the main production area of ​​bananas in my country, and it is also one of the origins of bananas. [0003] At present, almost all of the commercially valuable banana cultivars in my country are vegetatively fruity, with no seeds in the fruit or completely degraded seeds, belonging to triploids (including AAA genotype, AAB genotype and ABB genotype), with a high degree of male and female Due to the characteristic...

AI Technical Summary

Problems solved by technology

The commonly used medium is MS (Murashige and Skoog) medium, which needs to add macronutrients, trace element organic matter, iron salts, sucrose, hormones, agar and distilled water during preparation, and hormones are expensive, and plant growth is prone to dependence; and When preparing, the mother liquor needs to be prepared step by step, and the steps are cumbersome

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