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Plant tissue culture method for promoting strong roots of potato seedlings

A technology of plant tissue culture and potato, applied in the field of plant tissue culture, can solve the problems of weak growth of test-tube seedlings, affect the application effect of test-tube seedling cultivation efficiency, and underdeveloped root system, so as to promote strong roots of seedlings, easier transplanting and survival, and rooting The effect of effect promotion

Active Publication Date: 2018-09-21
WUHAN YEDONGLI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now the field cultivation of potatoes mainly adopts tuber propagation. Tuber propagation has many advantages, but there are also obvious disadvantages, that is, large amount of seed potatoes, low reproduction coefficient and easy accumulation of viruses, resulting in serious quality degradation.
[0004] And the test-tube plantlet that tissue culture obtains often grows way weaker, and root system is underdeveloped, and survival rate after transplanting is low, has a strong impact on the culture efficiency of test-tube plantlet and further application effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A plant tissue culture method for promoting strong roots of potato seedlings, comprising the following steps:

[0025] (1) Material collection and disinfection: In June, the top buds of potato seedlings growing vigorously in the field were cut off, the leaves were cut off, rinsed with tap water for 40-50 minutes, dried with sterile filter paper, and placed on an ultra-clean workbench with 75% Soak in alcohol for 25s, then sterilize with 0.1% mercury liter for 3-6min, and finally rinse with sterile water for 7-8 times, set aside;

[0026] (2) Stem tip stripping: under a binocular dissecting microscope, peel off the outer leaves of the sterilized potato terminal buds, leaving only 1-2 leaf primordia;

[0027] (3) Primary culture: Inoculate 5-6 shoot tips with a size of 0.1-0.2mm on the primary medium for cultivation; wherein the primary medium consists of: add 6-BA, ZT, Ethephon and uniconazole, the final concentration of 6-BA is 1.2mg / L, the final concentration of ZT is...

Embodiment 2

[0030] A plant tissue culture method for promoting strong roots of potato seedlings, comprising the following steps:

[0031] (1) Material collection and disinfection: In June, the top buds of potato seedlings growing vigorously in the field were cut off, the leaves were cut off, rinsed with tap water for 40-50 minutes, dried with sterile filter paper, and placed on an ultra-clean workbench with 75% Soak in alcohol for 25 seconds, then disinfect with 0.1% mercury liter for 3-6 minutes, and finally rinse with sterile water for 7-8 times, and set aside; add 2 drops of Tween 80 to the mercury liter solution;

[0032] (2) Stem tip stripping: under a binocular dissecting microscope, peel off the outer leaves of the sterilized potato terminal buds, leaving only 1-2 leaf primordia;

[0033] (3) Primary culture: Inoculate 5-6 shoot tips with a size of 0.1-0.2mm on the primary medium for cultivation; wherein the primary medium consists of: add 6-BA, ZT, Ethephon and uniconazole, the f...

Embodiment 3

[0036] A plant tissue culture method for promoting strong roots of potato seedlings, comprising the following steps:

[0037] (1) Material collection and disinfection: In June, the top buds of potato seedlings growing vigorously in the field were cut off, the leaves were cut off, rinsed with tap water for 40-50 minutes, dried with sterile filter paper, and placed on an ultra-clean workbench with 75% Soak in alcohol for 25s, then sterilize with 0.1% mercury liter for 3-6min, and finally rinse with sterile water for 7-8 times, set aside;

[0038] (2) Stem tip stripping: under a binocular dissecting microscope, peel off the outer leaves of the sterilized potato terminal buds, leaving only 1-2 leaf primordia;

[0039](3) Primary culture: Inoculate 5-6 shoot tips with a size of 0.1-0.2mm on the primary medium for cultivation; wherein the primary medium consists of: add 6-BA, ZT, Ethephon and uniconazole, the final concentration of 6-BA is 1.8mg / L, the final concentration of ZT is ...

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Abstract

The invention relates to the field of plant tissue culture, in particular to a tissue culture method for promoting strong roots of potato seedlings. The tissue culture method comprises the following steps: (1) carrying out material collection and disinfection; (2) stripping stem tips: stripping outer layer leaves of the sterilized potato apical buds under a binocular dissection mirror, and leavingonly 1-2 leaf primordia; (3) carrying out primary culture: inoculating 5-6 stem tips with sizes of 0.1-0.2mm in a primary culture medium for culturing; (4) carrying out rooting culture: inoculating the sterile seedlings, obtained in step (3), in a rooting culture medium in a form of single plants for culturing so as to obtain virus-free test-plantlets. The tissue culture method provided by the invention utilizes stem tip in vitro culture to solve the problem of large consumption of seed potatoes; furthermore, the primary culture medium, the rooting culture medium and culture conditions are optimized, so that the method not only can enable potatoes to have more roots and rapidly take roots, but also can enable the tissue cultured seedlings of the potatoes to be healthy and strong so as toenable the seedlings to be more easily transplanted and more easily survive.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a plant tissue culture method for promoting root strengthening of potato seedlings. Background technique [0002] Plant tissue culture technology is a technology developed in the early 20th century. It refers to the in vitro culture of isolated plant organs, tissues, cells, protoplasts, etc. under sterile conditions to induce callus, Adventitious buds, etc., followed by the process of forming a complete plant. Tissue culture technology, because of its advantages of controlling cell growth and differentiation under various artificial culture conditions, has effectively promoted the interdisciplinary development of plant physiology, pathology, pharmacy, genetics, breeding, and biochemistry, and has been widely used. In various industries such as agriculture, forestry, horticulture, industry, medicine, etc., it produces huge economic and social benefits. In summary, there are th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 陈道甫林心灵杨辉艳马星何子元
Owner WUHAN YEDONGLI BIOTECH CO LTD
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