Root induction method for Chinese fir test-tube plantlets
A technology for test-tube seedlings and Chinese fir, which is applied in the field of inducing rooting and culturing of Chinese fir plants, can solve problems such as difficult rooting of fir test-tube seedlings, and achieves the effects of overcoming the difficulty in inducing rooting of test-tube seedlings, improving the survival rate, and solving the difficulty in rooting.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] Induced rooting technique of Chinese fir test-tube seedlings
[0024] Experimental materials The materials come from the aseptic seedlings of Chinese fir in the state-owned forest farm of Laibin City, Guangxi, and have undergone 5 consecutive multiplication and cultivation of cluster buds.
[0025] Experimental equipment Ultra-clean workbench, tweezers, scalpel, sterile filter paper and medium containing two different hormones and blank medium. The whole experiment process was carried out in the cultivation room, the temperature was 24±1°C, and the light intensity was 2000-3000LX.
[0026] The Chinese fir test-tube seedling material treatment first selects the Chinese fir test-tube seedlings that grow robustly and has a size of 3.0-4.0 cm, and then cuts off 2-3 leaves at the base of the test-tube seedlings when inoculating (it is advisable that the leaves do not contact the medium when inoculating). During the inoculation process, do not leave the fir test-tube seedlin...
Embodiment 2
[0036] Effects of seedling hardening time on rooting of Chinese fir tissue culture seedlings on light substrate nutrient cups
[0037] High-concentration auxin stimulates and induces the rooting of Chinese fir test-tube seedlings with the same steps as in Example 1, and adopts the Chinese fir test-tube seedlings inoculated into the 2,4-D naphthalene acetic acid NAA auxin solution of 10 mg / L in embodiment 1 after stimulating treatment for 3d The test-tube seedlings were inserted into a blank medium and cultivated for 1 day (the blank medium specifically consists of the following raw materials in parts by weight: 50 g of MS medium, 3 g of diatom powder, 2.4 g of sucrose, and 0.25 g of konjac glucomannan. ), then this Chinese fir test-tube plantlet is processed 24h in the 2,4-D naphthalene acetic acid NAA auxin solution of 10mg / L, and the induced rooting Chinese fir test-tube plantlet that obtains carries out the next test:
[0038] Cultivate the rooted test-tube seedlings under ...
Embodiment 3
[0043] Effects of fly ash, sawdust and thiourea on the rooting of fir tissue culture seedlings on light substrate nutrient cups
[0044] High-concentration auxin stimulates and induces the rooting of Chinese fir test-tube seedlings with the same steps as in Example 1, and adopts the Chinese fir test-tube seedlings inoculated into the 2,4-D naphthalene acetic acid NAA auxin solution of 10 mg / L in embodiment 1 after stimulating treatment for 3d The test-tube seedlings were inserted into a blank medium and cultivated for 1 day (the blank medium specifically consists of the following raw materials in parts by weight: 50 g of MS medium, 3 g of diatom powder, 2.4 g of sucrose, and 0.25 g of konjac glucomannan. ), then this Chinese fir test-tube plantlet is processed 24h in the 2,4-D naphthalene acetic acid NAA auxin solution of 10mg / L, and the induced rooting Chinese fir test-tube plantlet that obtains carries out the next test:
[0045] The rooted test-tube seedlings were cultivate...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com