A method for rooting in a bottle of tissue cultured seedlings of Xianmai fragrant tea
A technology of showing pulse-flavored tea vegetables and tissue culture seedlings, applied in the field of plant tissue culture, can solve the problems of low rooting rate of tissue culture seedlings, insufficient supply of propagation methods, and large demand, so as to promote industrial production processes and reduce production costs , the effect of increasing the rooting rate
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Embodiment 1
[0018] A method for rooting in a bottle of tissue cultured seedlings of Xianmaixiang tea leaves, comprising the steps of:
[0019] (1) Select the subcultured Xianmaixiang tea vegetable tissue culture seedlings, and the selection standard is healthy Xianmaixiang tea vegetable seedlings with a height of 3-4cm, normal growth and no vitrification;
[0020] (2) Inoculate the selected subcultured Xianmaixiangcha seedlings on 1 / 4MS rooting medium;
[0021] The rooting medium includes 40 g / L of sucrose, 7 g / L of agar powder, and added indole-3-butyric acid with a concentration of 0.1 mg / L; the pH of the medium is 6.0, the culture temperature is 26 ° C, and the light is 3000 lx .
[0022] The root primordium grew out after 9 days of inoculation, and the root length was 3-4cm on the 17th day, and the statistical rooting rate was 95% (the rooting rate was the percentage of the number of rooted plants and the number of plants in the inoculated group).
Embodiment 2
[0024] A method for rooting in a bottle of tissue cultured seedlings of Xianmaixiang tea leaves, comprising the steps of:
[0025] (1) Select the subcultured Xianmaixiang tea vegetable tissue culture seedlings, and the selection standard is healthy Xianmaixiang tea vegetable seedlings with a height of 3-4cm, normal growth and no vitrification;
[0026] (2) Inoculate the selected subcultured Xianmaixiangcha seedlings on 1 / 4MS rooting medium;
[0027] The rooting medium comprises 35 g / L of sucrose, 6 g / L of agar powder, and added indole-3-butyric acid with a concentration of 0.2 mg / L; the pH of the medium is 5.9, the culture temperature is 25 ° C, and the light is 3000 lx .
[0028] Root primordium grew out after 8 days of seedling inoculation, and the root length was 3-4 cm on 16 days, and the statistical rooting rate was 96% (the rooting rate was the percentage of the number of rooted plants and the number of plants in the inoculation group).
Embodiment 3
[0030] A method for rooting in a bottle of tissue cultured seedlings of Xianmaixiang tea leaves, comprising the steps of:
[0031] (1) Select the subcultured Xianmaixiang tea vegetable tissue culture seedlings, and the selection standard is healthy Xianmaixiang tea vegetable seedlings with a height of 3-4cm, normal growth and no vitrification;
[0032] (2) Inoculate the selected subcultured Xianmaixiangcha seedlings on 1 / 4MS rooting medium;
[0033] The rooting medium includes 30 g / L of sucrose, 5 g / L of agar powder, and the addition of indole-3-butyric acid with a concentration of 0.3 mg / L; the pH of the medium is 5.8, the culture temperature is 24 ° C, and the light is 2500 lx .
[0034] After 5 days of inoculation, the root primordium grew out, and the root length was 3 to 4 cm on the 13th day, and the statistical rooting rate was 100% (the rooting rate was the percentage of the number of rooted plants and the number of plants in the inoculation group).
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