The invention discloses a method for artificial rapid propagation of orchid orchids by using embryogenic
callus, which comprises the following steps:
cutting open the sterilized and sterilized capsules, inoculating the seeds in the
germination medium, and waiting for healing After the wound tissue appeared,
embryo callus induction and proliferation, protocorm differentiation, growth and proliferation, rooting culture,
seedling hardening and
transplanting were carried out. The core of the present invention is that the embryogenic
callus is produced after the seed coat of Orchid orchid is broken, and then differentiated into protocorms; on this basis, the culture medium is optimized and adjusted, and the embryogenic callus is proliferated, the protocorm is differentiated,
Germination growth and proliferation are carried out simultaneously, which simplifies the
tissue culture process, and the three culture processes are carried out in one medium at the same time, which greatly improves the
reproduction efficiency. In addition, the invention has low cost, short period, high
seedling quality and
survival rate.