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A kind of cultivation method of Maca polyploid plant

A breeding method and polyploid technology, applied in the biological field, can solve the problems of low quality and yield of maca, achieve the effects of overcoming chimera phenomenon, saving land resources, and shortening the induction time

Inactive Publication Date: 2017-04-26
会泽枢康中草药种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for cultivating maca polyploid plants for the low quality and yield of existing maca, and to select and breed a batch of new germplasm of polyploid maca. Maca polyploid breeding lays the technical foundation, and can also provide polyploid seedlings with the same genotype background to meet the needs of artificial planting

Method used

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  • A kind of cultivation method of Maca polyploid plant
  • A kind of cultivation method of Maca polyploid plant
  • A kind of cultivation method of Maca polyploid plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Press 100 grains / pack of maca seeds with fine gauze, wash them under running water for 2 hours, put them on an ultra-clean workbench and soak them in 75% (volume ratio) alcohol for 60 seconds, and then wash them with 0.1% HgCl 2 (Hercuric chloride) (mass ratio) Sterilize for 8 minutes, and finally rinse with sterile water 10 times, each time not less than 2 minutes, then put it on sterile filter paper, open the fine gauze to absorb the surface moisture, and get the disinfection and sterilization treatment seeds; shake the vessel well throughout the disinfection process;

[0032] 2. Callus induction Insert the sterilized seeds obtained in step 1 into the following medium A:

[0033] B 5 basic medium

[0034] Sucrose 20000 mg / L

[0035] Agar powder 4800 mg / L

[0036] pH 5.6

[0037] Under the conditions of light intensity 1500-2000 lx, light time 10 h / d, and temperature controlled at 20±1°C, the seeds were germinated and cultivated;

[0038] 3. The seeds in the m...

Embodiment 2

[0067] Embodiment two: the processing such as selection, disinfection of Maca seed described in this embodiment, inoculation, culture method, step and culture condition are all identical with embodiment one, and each culture medium composition is different, specifically as follows:

[0068] (1) Medium A:

[0069] B 5 basic medium

[0070] Sucrose 20000 mg / L

[0071] Agar powder 4800 mg / L

[0072] pH 5.8

[0073] (2) Callus induction medium B:

[0074] B 5 basic medium

[0075] Naphthaleneacetic acid NAA 1.8mg / L

[0076] Zeatin ZT 0.1 mg / L

[0077] Colchicine 1.0 mg / L

[0078] Sucrose 20000 mg / L

[0079] Agar powder 4800 mg / L

[0080] pH 5.8

[0081] (3) Cluster bud induction and proliferation medium C:

[0082] B 5 basic medium

[0083] 6-Benzylaminopurine 6-BA 1.0 mg / L

[0084] Naphthaleneacetic acid NAA 2.0mg / L

[0085] Zeatin ZT 0.3 mg / L

[0086] Colchicine 0.2 mg / L

[0087] Sucrose 20000 mg / L

[0088] Agar powder 4800 mg / L

[0089] pH 5.8

[0090] (4)...

Embodiment 3

[0098] Embodiment three: processing such as selection, disinfection of maca seed described in this embodiment, inoculation, culture method, step and culture condition are all identical with embodiment one, and each culture medium composition is different, specifically as follows:

[0099] (1) Medium A:

[0100] B 5 basic medium

[0101] Sucrose 20000 mg / L

[0102] Agar powder 4800 mg / L

[0103]pH 5.4

[0104] (2) Callus induction medium B:

[0105] B 5 basic medium

[0106] Naphthaleneacetic acid NAA 2.0mg / L

[0107] Zeatin ZT 1.0 mg / L

[0108] Colchicine 2.0 mg / L

[0109] Sucrose 20000 mg / L

[0110] Agar powder 4800 mg / L

[0111] pH 5.4

[0112] (3) Cluster bud induction and proliferation medium C:

[0113] B 5 basic medium

[0114] 6-Benzylaminopurine 6-BA 1.5 mg / L

[0115] Naphthaleneacetic acid NAA 1.8mg / L

[0116] Zeatin ZT 1.0 mg / L

[0117] Colchicine 0.5 mg / L

[0118] Sucrose 20000 mg / L

[0119] Agar powder 4800 mg / L

[0120] pH 5.4

[0121] (4) Ro...

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Abstract

The invention discloses a method for cultivating maca polyploid plants. The method comprises the steps that disinfected maca seeds are inoculated into an empty B5 culture medium, after the seeds germinate, hypocotyledonary axes of the seeds are taken to be inoculated to a callus induction culture medium with high colchicine to be cultured, obtained calluses are transplanted to a tufted-bud induction and enrichment medium with low colchicine to be cultured, then subculture multiplication is carried out for three to four generations, and maca polyploid multiple shoots are obtained; when the polyploidy multiple shoots grow to 3 cm to 4 cm, single studs are cut off to be transplanted to a rooting medium, and maca polyploid test-tube plantlets can be obtained after culturing of 25 d; after hardening-seedling is carried out on the polyploid test-tube plantlets for 3 d according to the convention, the polyploid test-tube plantlets are transplanted into a floating tray with disinfection humus, and maca polyploid seedlings are obtained after culturing of 60 d. The method has the advantages that screening can be carried out during the callus period, the method is simple and high in inductivity, few chimeras exist, the death rate is low, the polyploid test-tube plantlets can be obtained through meristematic proliferation and obtained polyploid materials, the induction time is greatly shortened, and the induced polyploid plants are good in quality and high in stress resistance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for breeding new varieties of medicinal and edible plants, in particular to a method for cultivating polyploid Maca plants. Background technique [0002] Maca Lepidium meyenii The genus Brassicaceae (Brassicaceae) Lepidium ) is an annual herbaceous plant, native to the Andes in Peru at an altitude of more than 3500 m. It can grow normally in the unique environment of no fertilizer, lack of oxygen, large temperature difference between day and night, and long-term ice. Maca is often referred to as "Peruvian Ginseng" because it rivals ginseng in terms of nutritional content and energy and stamina-boosting properties. Because maca absorbs a large amount of soil nutrients during its growth, under the original farming conditions, the land after harvesting maca roots needs to be left fallow for several years before it can be planted again. Therefore, maca was once on the verge of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王枢
Owner 会泽枢康中草药种植有限公司
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