A kind of high-efficiency artificial seedling raising method of Passiflora high-quality hybrid

Abstract

The invention provides a high-efficiency artificial seedling-raising method of high-quality passionflower hybrids, which comprises the steps of: inoculating the sterilized leaf explants in a culture medium, inducing callus, forming clustered buds and proliferating , rooting induction, seedling hardening and transplanting. The present invention optimizes and adjusts the production process of hybrid seedlings of passionflower, synchronously carries out callus growth, cluster bud generation and cluster bud proliferation, simplifies the tissue culture process, and carries out three culture processes in one culture medium at the same time , greatly improving the reproductive efficiency. In addition, the invention solves the phenomena such as plant yellowing and leaf defoliation that are easy to occur in the tissue culture process of Passiflora plants, and has low cost, short cycle, high quality and high survival rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for high-efficiency artificial seedling cultivation of high-quality passionflower hybrids. Background technique [0002] Passiflora coerulea L., also known as passion fruit, egg fruit, Brazil fruit, is a perennial herbaceous to semi-woody vine climbing tropical and subtropical plant of Passifloraceae (Passifloraceae) Passiflora (Passiflora); Originated in South America from Brazil to Argentina, my country is mainly distributed in Taiwan, Guangdong, Fujian, Guangxi, Zhejiang, Sichuan, Yunnan and other provinces and regions. Passiflora is evergreen in four seasons, with gorgeous flower color, 5 petals, light green, outer corona lobes are filamentous, and inner corona fringe, its fruit is berry ovoid to nearly spherical, orange or yellow when ripe, good for storage Good luck, good taste, with the reputation of "king of juice". [0003] Passiflora is a cross-pollina...

AI Technical Summary

Problems solved by technology

Traditionally, Passiflora is mainly propagated by seeds and cuttings. Seed seedlings have problems such as low germination rate, long seedling period, low survival rate, large separation of characters, and low robustness; while cuttings also have long cycle and Low survival rate and other problems, and long-term asexual reproduction is easy to cause virus accumulation, resulting in the degradation of passionflower species, the decline in variety quality, and the production decline year by year

These unfavorable factors have restricted the development of passionflower planting and processing industry, making my country's passionflower have no market competitiveness in the world

In Xishuangbanna Manfeilong Farm, Yunnan, a hybrid F 1 During the generation, a hybrid (new variety has not yet been applied for) has been selected with strong disease resistance, self-compatibility, purple-red fruit, fruit setting rate of more than 70%, and strong fruit juice fragrance and high sweetness; The variety is very unstable, and the above-mentioned excellent traits cannot be fixed by sexual reproduction.

There are many reports on tissue culture of various varieties of Passiflora, but there are still some problems in terms of reproduction coefficient and cycle, yellowing and defoliation of bottle seedlings, transplanting survival rate and high cost, so that the overall application of tissue culture seedlings is still at a low level. Level

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