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The method of overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flaxa grandiflorum

A large-flowered flax grass, artificial technology, applied in the directions of gardening methods, botanical equipment and methods, gardening tools/equipment, etc., to achieve the effects of developed root system, high transplant survival rate, and improved phenomenon of burning tip necrosis

Active Publication Date: 2021-05-07
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defects in the above-mentioned existing propagation techniques, and provide a method capable of improving the rapid propagation efficiency of the flax herb, which lays a technical foundation for the protection of its wild resources and the development of artificial planting. Fixed to provide high-quality seedlings with consistent genotype background to meet the needs of artificial planting

Method used

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  • The method of overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flaxa grandiflorum
  • The method of overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flaxa grandiflorum
  • The method of overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flaxa grandiflorum

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Embodiment 1

[0069] A method for overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flax Radix, comprising the following steps:

[0070] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformities, and take the lower stem section of the shoot tip, including the 4th to 6th nodes, 5.9cm long.

[0071] 2. Wash the surface dust and sundries of the knotted stem section of step 1 with tap water first, then soak it in 10% washing powder solution (mass ratio) for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, and place it on the ultra-clean workbench. Disinfect with 75% alcohol by volume for 15s, and then use 0.1% HgCl by mass 2 Sterilize for 6 minutes, and finally rinse with sterile water 5-6 times, each time not less than 3 minutes. Shake the dish well throughout the sterilization process.

[0072] 3. Induce the germination of axillary buds, the occurrence and pro...

Embodiment 2

[0086] A method for overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flax Radix, comprising the following steps:

[0087] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformity, and take the lower stem section of the shoot tip, including the 4th to 6th nodes, 6cm long.

[0088] 2. Wash the surface dust and sundries of the knotted stem section of step 1 with tap water first, then soak it in 10% washing powder solution (mass ratio) for 10 minutes, shake it slightly, rinse it with running water for 30 minutes, and place it on the ultra-clean workbench. Disinfect with 75% alcohol by volume for 15s, and then use 0.1% HgCl by mass 2 Sterilize for 6 min, and finally rinse with sterile water for 5 times, 3 min each time. Shake the dish well throughout the sterilization process.

[0089] 3. Induce the germination of axillary buds, the occurrence and proliferation of basal clum...

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Abstract

The invention discloses a method for improving the efficiency of artificial rapid propagation of flaxgrass overcoming burnt tip necrosis, which belongs to the field of biotechnology, and comprises the following steps: inoculating a sterilized and sterilized explant of a segmented stem segment into a culture medium, Induce the germination of axillary buds, the occurrence and proliferation of clustered buds on the base stems, overcome the necrosis of burnt tips, rejuvenate and take root, and transplant hardened seedlings. The invention completely solves the phenomenon of burning tip necrosis common in in vitro rapid propagation of plants of the family Scrophulariaceae, especially of the genus Flax, and has low cost, short cycle, high seedling quality and survival rate. In addition, the production process of aseptic seedlings of C. grandiflorum was optimized and adjusted. While improving burn tip necrosis, axillary bud germination and growth, basal stem cluster bud induction and growth were simultaneously carried out, and the tissue culture process was simplified. Three culture processes At the same time in a culture medium, greatly improving the reproduction efficiency.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the efficiency of artificial fast propagation by overcoming burning tip necrosis. Background technique [0002] Centranthera grandiflora Benth. is Scrophulariaceae ( Scrophulariaceae ) plants of the genus Centranhera, erect and sturdy herbs, 50-80cm high; mainly produced in Yunnan, Guizhou, Guangxi and other provinces, born on hillsides, roadsides and open spaces at an altitude of 800m, and also distributed in Vietnam, Myanmar, and India. [0003] "Chinese Materia Medica" records: Dahua flax grass is used as medicine, fresh or dried, commonly known as red root wild bean, Xiaohongyao, Huaxuedan, Ganoderma lucidum, golden cathead; sweet, light; warm in nature; Promotes blood circulation and regulates menstruation, disperses blood stasis and relieves pain; used for amenorrhea, dysmenorrhea, metrorrhagia, bruises, rheumatism and bone pain; externally u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 徐福荣黄衡宇
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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