Tissue cultivaition method of vetiveria zizanioides transplant

Abstract

The invention provides a tissue cultivation method of vetiveria zizanioides transplants. The method comprises steps that: sterilizated vetiveria zizanioides explants are placed in induction and proliferation culture media; inducting and proliferation cultivating are carried out concurrently until explants are differentiated and young buds are germinated; times of subcultures are carried out untila required number of seedlings are obtained; the seedlings are transplanted into cultivation media to be cultivated until 3 to 5 roots appear; the seedlings are then transplanted into cultivation bags; routine managements of watering and fertilizing are carried out, and vetiveria zizanioides transplants can be obtained after 40 days of growth. With the method provided by the present invention, usage amounts of drugs can be reduced. Through the concurrent inducting and proliferation cultivating, appearances of variants can be efficiently controlled, tissue cultivating processes can be simplified, propagation periods can be shortened, production cost can be reduced, and merits of vetiveria zizanioides varieties can be kept fast and stably. With the method provided by the present invention, problems in methods with common propagation systems, such as complex parent resources, unstable seedling qualities and slow seedling propagations, can be solved.

Description

technical field [0001] The invention relates to a method for tissue culture of transplanted seedlings of vetiver, and belongs to the field of biotechnology. Background technique [0002] Vetiver (Vetiveria zizanioides) is a perennial herb of the Poaceae Vetiver, native to Southeast Asia, India and tropical Africa. The vetiver grass grows densely on the ground, with a stalk height of 1-2m; the leaves are strip-shaped, hard, and 4-10cm wide; it blooms in autumn and generally has no fruit; the deep root system can generally reach 2-3m deep, and can adapt to various soil environments , can grow under conditions such as strong acid and strong alkali, heavy metals, drought, waterlogging, and barrenness. It entered China in 1958 and was planted in Guangdong, Fujian and other places. It is generally used as a spice plant to extract the aromatic oil from the roots and leaves. Since the 1980s, vetiver has been widely used to control soil erosion in South China, East China, and South...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the existing vetiver seedling propagation technology existing problems such as high material requirements, large dosage, difficulty in large-scale production, etc., the object of the present invention is to provide a kind of vetiver The root grass industrialized tissue culture and rapid propagation method improves the production process and medium formula to increase the proliferation rate and reduce production costs. At the same time, the seedlings with excellent traits are fixed, and the operation is simple and easy to meet the needs of high-quality seedlings. mass production needs