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A kind of tissue culture breeding method of P.

A technology of tissue culture and qinpu, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficulty in large-scale production, slow reproduction speed and high material requirements, and achieve stable seedling traits and reproduction speed. Faster and more economical

Active Publication Date: 2019-05-28
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to overcome the traditional methods of breeding seedlings of existing rhizome propagation, resulting in slow propagation speed, low propagation coefficient, high material requirements, large consumption, and difficulty in large-scale production. Defects and deficiencies, thereby providing A method suitable for industrial tissue culture and rapid propagation of P. chinensis and a special medium to meet the needs of large-scale production of high-quality P. chinensis seedlings

Method used

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  • A kind of tissue culture breeding method of P.
  • A kind of tissue culture breeding method of P.
  • A kind of tissue culture breeding method of P.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) if figure 1 As shown, the shoot tips germinated on both sides of the healthy Rhizoma chinensis were taken as explants, cleaned with washing powder, rinsed with flowing tap water for 1 hour, and then soaked with 72% streptomycin sulfate for 3 hours. After soaking in 75% alcohol for 40 seconds in an ultra-clean environment, put it into 0.1% mercuric chloride solution for disinfection for 20 minutes, two drops of Tween, 15 minutes of disinfection in sodium hypochlorite mixture, Tween Two drops, then rinse with sterile water 4 to 5 times, each time for 1 to 2 minutes; wherein the sodium hypochlorite mixture is formed by adding sodium hypochlorite solution to Tween 20, and the ratio of sodium hypochlorite solution to Tween 20 is: Add 2 drops of Tween 20 to 100 ml of sodium hypochlorite solution with a mass fraction of 2%.

[0041] (2) Induction and proliferation culture: such as figure 2 As shown, the aseptic shoot tip of the germination of the lateral plant of the pa...

Embodiment 2

[0054] (1) Embodiment 2 is the same as Embodiment 1 except that the following measures are different, and will not be repeated.

[0055] Take the shoot tips germinated on both sides of the healthy Rhizoma chinensis as explants, soak them in alcohol with a mass fraction of 75% for 30s in an ultra-clean environment, and then put them in a mercuric chloride solution with a mass fraction of 0.1% for disinfection for 18 minutes , two drops of Tween, disinfected in sodium hypochlorite mixture for 16 minutes, and two drops of Tween.

[0056] (2) Induction and proliferation cultivation: the aseptic shoot tip of the germination of the lateral plant of the Rhizoma chinensis rhizome obtained in step (1) is inserted in the culture bottle filled with the following induction proliferation medium:

[0057] MS Basic Medium

[0058]

[0059] Cultivate for 15 days at a temperature of 23° C., with a light intensity of 1800 lx and a light time of 10 h / d, cultivate until shoot tip shoots grow,...

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Abstract

The invention discloses a tissue culture breeding method for acorus gramineus. The tissue culture breeding method comprises the steps of explant disinfection, induction, enrichment culture, subculture, rooting culture, seedling hardening and transplanting. According to the method, the acorus gramineus is high in reproduction speed, and roots and seedlings are robust; the cost and the production time are greatly saved; the problems that the source of parents of seedlings produced by a conventional breeding system is complicated and the quality of the seedlings is unstable; the tissue culture breeding method is suitable for standard, industrial and large-scale production; high-quality seedlings of the uniform standard are provided for the market; the problem that acorus gramineus seedlings cannot be yearly produced is solved.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a method for tissue culture and breeding of pachyrhiza. Background technique [0002] Acorus gramineus Soland is a perennial emergent herbaceous plant of the genus Iris in the family Araceae, with clusters of branches. Produced in Jiangsu, Zhejiang, Jiangxi, Hubei, Hunan, Guangdong, Guangxi, Shaanxi, Gansu, Sichuan, Guizhou, and Yunnan. It is evergreen, with emerald green and shiny leaves, dignified and beautiful, with fragrance, suitable for waterscape shore and water greening. It can also be used as a potted ornamental plant or as a scenery. The leaves and inflorescences can also be used as flower arrangement materials. The whole plant is fragrant and can be used as spice or mosquito repellent; the rhizome can be used as medicine, with pungent taste and warm nature. Dispelling dampness and whetting the appetite, resuscitating the body and eliminating phlegm, refres...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 苏艳桂敏瞿素萍张艺萍王丽花张丽芳杨秀梅许凤吴旻
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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