Method for cultivating seedlings of polygonatum kingianum

Abstract

The method for raising seedlings of Rhizoma Polygonatum, the steps include: preparation of explants of Rhizoma Rhizoma Diannensis, culture for eliminating browning, callus induction, adventitious bud generation and proliferation culture, dark culture for promoting rhizome growth, rejuvenation culture of test-tube plantlets, rooting culture, hardening of seedlings Cultivation; the beneficial effect is: the Yunnan gold explants are carried out in the substratum A in the transfer culture of every 7 days, and the effect of removing the browning phenomenon occurring in the tissue culture process is remarkable; simultaneously carry out callus induction, cluster bud generation and Proliferation culture improves the reproduction efficiency, the reproduction coefficient can be increased to more than 8.67, and the tissue culture time can be shortened to 45 days; in the dark culture stage, the rhizome of Polygonatum yunnanensis is greatly improved, and the survival rate can reach more than 95%; the invention is divided into stages Four kinds of media are used for transfer culture, which overcomes the problem of easy breeding of fungi and accumulation of viruses when Polygonatum yunnanensis is used for group cultivation of seedlings. The cultivated seedlings have strong adaptability and stress resistance, which reduces the pollution rate of group cultured seedlings and improves To ensure the stability of species.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of traditional Chinese medicinal materials, and relates to a seedling raising method of Polygonatum yunnanensis. Background technique [0002] Polygonatum kingianum Coll.er Hemsl. is a perennial herb of the Liliaceae genus Polygonatum, mainly produced in Yunnan, Sichuan, Guizhou and Vietnam, rarely distributed in Myanmar; born in forest shrubs Or damp grass slopes, rocks. Dianhuangjing is a traditional Chinese medicinal material. Its medicinal part is the rhizome. It is flat in nature and sweet in taste. It is a nourishing top grade. Less dry food, lung deficiency and dry cough, lack of essence and blood, internal heat and thirst. Modern pharmacological studies have shown that Rhizoma Polygonatum also has the functions of immune stimulation, immune enhancement, anti-aging, anti-virus, etc. It has broad prospects in the research of new drugs and the development of health care products. Du...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of slow propagation speed, easy browning, long cultivation period, easy breeding of fungi and accumulation of viruses in propagules such as rhizomes in the propagation of Rhizoma Polygonatum in the prior art, resulting in the degradation of species, the present invention provides a Rhizoma Polygonatum Seedling raising method, concrete scheme comprises the following steps: