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A kind of culture medium for cultivating the mother species of wild reticulata fringe

A culture medium and crimping technology, which is applied in cultivation, application, plant cultivation, etc., can solve the problems of restricting industrial development, teaching and scientific research, and the inability to artificially cultivate wild reticulum crimpingum, and achieve good application prospects and great economic benefits. Value and social benefit, effect of simple preparation method

Inactive Publication Date: 2020-12-22
YANGTZE NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the gaps in the prior art, the purpose of the present invention is to provide a culture medium for cultivating the wild Dicticholium cringularis parent species, to solve the problem that the wild Dictophyllum cringularia cannot be artificially cultivated, which greatly limits its industrial development and teaching and research issues

Method used

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  • A kind of culture medium for cultivating the mother species of wild reticulata fringe
  • A kind of culture medium for cultivating the mother species of wild reticulata fringe
  • A kind of culture medium for cultivating the mother species of wild reticulata fringe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The culture medium used in this example for the cultivation of the wild Reticula pleinosa parent species: 20 g of soluble starch, 2 g of peptone, KH 2 PO 4 3.0g, MgSO 4 4.5g, water 1L, pH 4.5.

[0072] 1) Under a sterile environment, cut out about 0.3 cm of the internal tissue block of the fruiting body of the wild Dictyophyllum cringulata and inoculate it in the central part of the PDA slant medium, plug the rubber stopper, mark the name of the strain and the date of inoculation, and place it at 25 After 3-5 days of dark-light cultivation in an incubator at ℃, brown fluffy hyphae germinated from the tissue block. When the mycelium grew to 2-3 cm away from the tissue block, the edge hyphae were picked and transferred to a blank PDA. On the slant medium, when the test tube is overgrown, the purified wild Dictyophyllum cringulae parent species can be obtained;

[0073] 2) Inoculate the purified wild Reticulophyllum cringulata parent species on the PDA plate medium, c...

Embodiment 2

[0077] The culture medium used in this example for the cultivation of the wild Reticula pleurata parent species: 15 g of soluble starch, 2 g of peptone, KH 2 PO 4 1.0g, MgSO 4 0.5g, 1L of water, pH 4.5.

[0078] 1) Under a sterile environment, cut out about 0.3 cm of the internal tissue block of the fruiting body of the wild Dictyophyllum cringulata and inoculate it in the central part of the PDA slant medium, plug the rubber stopper, mark the name of the strain and the date of inoculation, and place it at 25 After 3-5 days of dark-light cultivation in an incubator at ℃, brown fluffy hyphae germinated from the tissue block. When the mycelium grew to 2-3 cm away from the tissue block, the edge hyphae were picked and transferred to a blank PDA. On the slant medium, when the test tube is overgrown, the purified wild Dictyophyllum cringulae parent species can be obtained;

[0079] 2) Inoculate the purified wild Reticulophyllum cringulata parent species on the PDA plate medium...

Embodiment 3

[0083] The medium used in this example for the cultivation of the wild Reticula pleurata parent species: 15 g of soluble starch, 4 g of peptone, KH 2 PO 4 3.0g, MgSO 4 1.5g, water 1L, pH 4.5.

[0084] 1) Under a sterile environment, cut out about 0.3 cm of the internal tissue block of the fruiting body of the wild Dictyophyllum cringulata and inoculate it in the central part of the PDA slant medium, plug the rubber stopper, mark the name of the strain and the date of inoculation, and place it at 25 After 3-5 days of dark-light cultivation in an incubator at ℃, brown fluffy hyphae germinated from the tissue block. When the mycelium grew to 2-3 cm away from the tissue block, the edge hyphae were picked and transferred to a blank PDA. On the slant medium, when the test tube is overgrown, the purified wild Dictyophyllum cringulae parent species can be obtained;

[0085] 2) Inoculate the purified wild Reticulophyllum cringulata parent species on the PDA plate medium, culture i...

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Abstract

The invention discloses a medium for cultivating a wild paxillus involutus stock culture. The medium is prepared from, by mass, 15-25g of soluble starch, 2-6g of peptone, 1-5g of KH2PO4, 0.5-4.5g of MgSO4, 1L of water and 2-6 of pH. The medium has the advantages of early germination, rapid growth, good growth, high mycelial biomass, short production cycle and the like, the biological characteristics of the bacterium are mastered, and a foundation is laid for further development of an application value and artificial domestication and cultivation. The successful cultivation of the stock culturealso provides new germplasm resources for the development and utilization of medicinal ingredients and teaching and researching, and has great economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of edible and medicinal fungus cultivation, and in particular relates to a culture medium for cultivating wild Dictyophyllum cringulatum parent species. Background technique [0002] Paxillus involutus (Batsch) Fr. belongs to Basidiomycota, Hymenomycetes, Agaricales, Paxillaceae, and Paxillus. The Chinese aliases are curled edge (edge) pileus, roll mushroom, roll edge reticulum, and drop pleura. Distributed in Hebei, Beijing, Jilin, Heilongjiang, Liaoning, Fujian, Shanxi, Ningxia, Anhui, Hunan, Guangdong, Sichuan, Yunnan, Guizhou, Tibet and other places. Growing solitary or in groups on tree stumps, or forming clumps from underground tree stumps. In summer and autumn, it often grows on poplars (Mao Xiaolan, Chen Yumei, He Jian. Hulunbeier Mushroom Picking Industry[J]. Edible Fungi, 1987, (3): 1~2), oak and birch and other understory stakes . It forms mycorrhiza with poplar, willow, larch, spruce, pine, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/20A01G18/50
CPCA01G18/20A01G18/50
Inventor 杜萍曹天旭凃慧陈今朝蒋佳颖
Owner YANGTZE NORMAL UNIVERSITY
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