The invention provides a nano-scale pressure-reducing, injection-flooding, oil displacement and viscosity-reducing integrated agent and its preparation method and application. The raw material composition of the integrated agent includes: 5.0%-10.0% oil-soluble comb polymer, 5.0%-10.0% Triethylenetetraminehexaacetic acid, 8.0%‑10.0% dihexyltriaminepentamethylene phosphate, 8.0%‑10.0% monoethanolamine, 5.0%‑10.0% cocamidopropyl mesylate beet Alkali, 20.0%‑25.0% xylene, 3.0%‑5.0% dioctadecyldimethylammonium chloride, 10.0%‑15.0% nonylphenol ethoxylate TX‑15, 15.0%‑20.0 % of methanol and the balance of water. The present invention also provides a preparation method of the above-mentioned integrated agent. The nano-scale pressure-reducing, increasing-injection, oil-flooding and viscosity-reducing integrated agent of the present invention is used as an additive for water injection wells, and the dosage is 0.1%-0.3%, which can effectively reduce the oil-water interfacial tension to 10 ‑3 At mN / m level, it can effectively eliminate formation clogging caused by barium, strontium sulfate and organic scale in the formation, and at the same time, it can effectively reduce the viscosity and freezing point of crude oil by more than 60%, and effectively improve the production efficiency of oil wells.