The invention provides feed for promoting estrus and pregnancy of female forest musk deer. The feed is prepared from seeds of Chinese dodder, rhizoma cyperi, epimedium, prepared rehmannia root, herba lycopi, leonurus, patrinia and corn starch. If female forest musk deer eats the feed in the mating preparation period, the pregnant rate and the rate for having multiple births can be increased. The invention also provides a method for breeding female forest musk deer with the feed. According to the method, a proper amount of feed is fed to female musk deer in the mating preparation period, after the feed is fed for 20 to 40 days, the illumination time and temperature of a female musk deer living environment are controlled, the temperature and the illumination are regularly changed, the temperature is gradually reduced, the illumination time is gradually shortened, and the pregnancy rate and the rate to have multiple births of female musk deer can be remarkably increased.