The invention discloses a method for establishing a
bladder cancer in-situ model of a
nude mouse. The method is characterized by comprising the following steps of: narcotizing a female
nude mouse superficially, and inserting a
urethral catheter with a side hole into a bladder from a urethral canal, so that negative pressure is formed in the
urethral catheter continuously; penetrating a cutter into the
urethral catheter, and adjusting the positions of a
cutting inclined plane and the side hole of the urethral
catheter;
cutting under the negative pressure to
cut part of
mucous membrane layers off, so that the lower layer of a bladder
mucous membrane is exposed to form a local
wound surface; and pouring
human bladder cancer cell suspension which is in the logarithm growth period into a
bladder cavity of the
nude mouse and reserving for a period of time, discharging the
cell suspension out, and establishing the superficial
bladder cancer model after 7 days. Compared with the conventional electrocauterization method, the conventional chemical medicinal
corrosion method and the conventional bladder puncture intracavity
cutting method, the method has the advantages that molding assemblies are low in production cost, and the method for establishing the
bladder cancer model is good in effect, the device is easy to operate, the time is short, the nude mouse has little pain, and death caused by
anesthesia, operation strike and infection is reduced obviously.