47 results about "Larvacea" patented technology

The invention discloses an interspecific hybridization and propagation method for deep sea scallops and chlamys farreri. The interspecific hybridization and propagation method comprises the following steps: a first step of breeding parent scallops: selecting deep sea scallops and chlamys farreri, separating the male and the female of the two scallops respectively, promoting maturity according to a normal parent scallop breeding method and using the mature scallops for cross experiment after synchronously maturing the mature scallops; a second step of laying eggs and performing hybridization: inducing spawning of the male and female groups of the two scallops respectively, fertilizing obtained eggs with conspecific or heterogeneous sperms respectively to obtain pure-bred self-bred progenies of the deep sea scallops and chlamys farreri as well as two hybrid fertilized eggs of female deep sea scallops and male chlamys farreri, female chlamys farreri and male deep sea scallops; hatching the obtained fertilized eggs at 13 to 14 DEG C, observing and measuring the proportion of D type larvas at regular times after the D type larvas appear until the maximum hatching rate is reached; a third step of cultivating scallops, wherein at a larval phase, the average shell height growth speed of the hybrid scallops is 6 to 6.3m per day, and the shell height, the shell length, the shell width and the weight are increased in a cultivating phase. According to the interspecific hybridization and propagation method disclosed by the invention, the deep sea scallops are effectively utilized through interspecific hybridization, so that the scallop breeding species are increased.

The invention relates to a breakwater ecologicalization technology, particularly to a servicing ocean breakwater underwater area ecological restoration method, and belongs to the field of ocean engineering and ocean ecological engineering. The method comprises the following steps: (1) investigating a sea area of a concrete structure construction position; (2) preparing a concrete adhesion matrix;(3) quantitatively collecting and culturing young oysters; (4) cleaning the surface of the breakwater; (5) carrying out underwater pouring of mortar; (6) placing an oyster adhesion matrix on site; (7)monitoring larva attachment; and (8) adding and managing nutrient substances. According to the invention, underwater non-dispersible ecological mortar is developed, and is poured into breakwater underwater area without oyster attachment to induce oysters to be massively attached to the breakwater underwater area, and due to the fact that the oysters serving as ecological engineers have the characteristics of purifying water and improving the water area, ecological restoration of the breakwater underwater area is achieved, and all-around high ecologicalization of the marine breakwater is achieved.

The invention discloses a preparation method of a Crassostrea hongkongensis fixation medium. The preparation method comprises the following steps that (1) a plurality of rectangular plastic sheets are made and a round hole with the radius being 3-5 mm is formed in each plastic sheet, wherein the length of the plastic sheets is 10-20 cm, the width of the plastic sheets is 2-5 cm and the thickness of the plastic sheets is 1-3 cm; (2) the plastic sheets are strung through a rope, a plastic tube is arranged between every two adjacent plastic sheets, the plastic tubes are arranged on the rope in a sleeved mode, and then the preparation process of the Crassostrea hongkongensis fixation medium is completed. The invention further discloses the Crassostrea hongkongensis fixation medium and an application method of the Crassostrea hongkongensis fixation medium. After being processed through oxalic acid, fresh water and the like, the Crassostrea hongkongensis fixation medium is used as an eyespot larva collection fixation medium; the Crassostrea hongkongensis fixation medium increases the metamorphosis rate of Crassostrea hongkongensis larvas, accelerates juvenile mollusk growth, improves the juvenile mollusk survival ability and increases the juvenile mollusk yield, has the advantages of being capable of being recycled multiple times, light, convenient to use, good in corrosion resistance and the like and is worthy of popularization and application.

A method of artificial breeding for ark shells, which includes cultivating unicellular algae first, then breeding, sampling and inspecting parent calms gonads of ark shells in the sea, capturing and transporting parent calms, breeding species shells temporarily in seeding room interiorly; spawning and hatching parent calms; selecting and breeding larvae, cultivating larvae, changing water once a day, scattering bait 30,000-100,000 / ml single cells every day; scattering cultch when the body length of larvae is 230 micrometers, changing water totally every day, moving larvae to the middle of the sea or shrimp tank to breed temporarily when the shells of them are 0.7-1.2mm; beating platform raft firstly as temporary breeding, the direction of which and flow direction being almost vertical; temporary breeding management, tying one hanging at intervals of 0.6-1.0m on every platform raft, 6-10 bags on every hanging, 5,000-15,000 juveniles shellfishes in every bag, arranging platform raft, promising the safety of platform raft, scattering on the bottom of the sea or cultivating in the shrimp tank when juveniles shellfishes grow up above 5mm. The advantage of the method can realize artificial breeding, short time of breeding, cultivation in the shrimp tank, bottom sowing in the sea and resume the population advantage, low-cast, quick results and great benefit.

The invention discloses a Ruditapesphilippinarum seedling large scale cultivation method, belongs to the aquatic product cultivation field, and the method comprises the following steps: reclamation area sand blowing and disinfection, parent selection and spawning induction, planktonic larva cultivation, adhesion juvenile mollusk cultivation, and sand seedling harvesting; by using the method, 50,000,000-120,000,000 Ruditapesphilippinarum sand seedlings (shell length 1.5-4mm) can be grown in each mu; the method can be applied to coastal area in our nation, thus realizing Ruditapesphilippinarum seedling large scale and industrialization production, and providing seedling guarantee for developing Ruditapesphilippinarum cultivation industry; the method is simple in operation and easy in promotion.

The invention relates to a killifish culture method. The method includes the following steps: culture water, the pH of which is 6.5 to 8.0 and the oxygen content of which is 4mg / L to 8mg / L, is adopted to incubate spawns under 24 DEG C to 30 DEG C for 4 to 12 hours, incubated larvae are fed with brine shrimp larvae after 10 to 15 hours, water is changed every three days, and the amount of changed water is 1 / 10 to 1 / 5 each time; after 10 to 20 days, the larvae are grown into adult fishes, the adult fishes are transferred into a culture tank for raising and fed with fairy shrimps, water is changed every two days, and the amount of changed water is 1 / 8 to 1 / 4 each time. The culture method for culturing killifishes is easy to operate, and moreover, both the incubation rate and the survival rate of the killifishes are high.

The invention relates to a breakwater ecologicalization method, in particular to an on-service ocean breakwater ecologicalization method, and belongs to the field of ocean perphyton and ocean ecological engineering interdisciplinary combination. The on-service ocean breakwater ecologicalization method comprises the steps of: (1) sea area investigation in a building position of a concrete structure; (2) preparation of a concrete substratum; (3) quantitative acquisition and culture of oyster seedlings; (4) surface cleaning of a breakwater; (5) brushing and curing of paint; (6) scene placement ofan oyster substratum; (7) monitoring of larva attachment; and (8) adding and management of nutrient substances. Through cleaning of the on-service breakwater and fixed placement of the stratum attached with oysters around the breakwater, the compact oysters are attached on the on-service breakwater more quickly; and through the characteristic of using the oysters as engineers and the effects of purifying water and improving a water area, high ecologicalization of the on-service breakwater is achieved, and the purposes of ecologicalization and long-time on service of the breakwater are achieved.

The invention relates to an ecological breakwater technology, in particular to a construction method of an ecological riprap breakwater, and belongs to the field of marine ecological engineering. Themethod comprises the following steps of (1) surveying a sea area wherein a breakwater construction position is located; (2) preparing a concrete attachment substratum; (3) quantitatively collecting and breeding oyster seedlings; (4) carrying out stone block surface treatment; (5) carrying out stone block placement; (6) placing the oyster attachment substratum on site; and (7) monitoring larva attachment and carrying out management. According to the construction method, the reasonable space layout is adopted, so that all stone piles (blocks) can effectively dissipate waves when the ocean current load is large, and water bodies on the two sides of the breakwater are smoothly exchanged at ordinary times; and after the oysters attached to all the stone piles (blocks) are massively bred, the ecological breakwater can purify the water bodies and improve the ecological environment of the surrounding sea area.

The invention relates to a marine sessile organism corrosion prevention technology, and particularly relates to an ecological corrosion prevention method for a serving marine reinforced concrete engineering, and belongs to the field of marine sessile organism and marine engineering interdisciplinary. The method comprises the steps of concrete structure construction position sea area investigation,oyster eyespot larva culture, concrete surface cleaning, concrete structure construction, coating and curing, field attachment and larva attachment monitoring and management. A cement-based coating for inducing sessile organisms is adopted, the characteristics of being capable of rapidly inducing attachment and metamorphosis of the sessile organisms and promoting long-term growth are achieved, the characteristics of being easy to construct and easy to coat are achieved, the novel marine sessile organism corrosion prevention technology for reinforced concrete engineering in the marine environment is developed, the durability of reinforced concrete can be greatly improved, meanwhile, the ecological environment of the sea area is improved, the ecological environment current situation of serving engineering is changed, and the damaged ecological environment is repaired.

The invention discloses an abalone fry cultivation method. The method comprises that: A. Benthic diatoms are cultured under strong light 2000-3000lx. B. The larvae of abalone are cultured directly in 5-10 square strip ponds, and then the sieve was selected and counted. C. According to the counting results, the number of abalone seedlings in floating period is determined, and cultured in the cultivation pond of pre-placed benthic diatom according to certain density. The cultivation method changes the time of the larvae of the abalone enter a large pool in the prior art, and therefore the cultivation of planktonic larvae is conducted in large space and low density, mechanical damages of single screen dragging selection is reduced, the dilemma of deaths of a large number of abalone larvae in the period of metamorphosis is well solved, the survival rate is improved, and the metamorphosis rate of larva is improved. The costs of abalone repurchase, nurturing, spawning and personnel employment is reduced, and the production cost is reduced.

A method for preserving marine aquarium foodstuffs such as zooplankton crustaceans, gelatinous organisms, vertebrate and invertebrate larvae and eggs, shellfish, mollusks, fish and fish roe, oysters and clams, and sea urchins, wherein a supersaturated saline solution is prepared from reverse osmosis deionized water and marine salt, said solution is chilled to a low temperature, and said foodstuffs are immersed into said solution such that moisture is withdrawn from said foodstuffs via osmosis. The resulting product may then be stored and shipped at low temperatures for subsequent rehydration and use as a food product for a salt water aquarium ecosystem.

A tray system suitable for use with fish laying eggs in skeins provides a set of cassettes having opposed water-permeable faces that may clamp about the edges of the skeins to prevent them from coiling about themselves while exposing both sides of the skeins to flowing water. A regular form factor of the cassettes allows them to be closely stacked with small water flow gaps between them for high hatching density.

The invention provides an induction method of a high-heterozygosity tetraploid of a new variety of crassostrea gigas 'Haida No.3'. The method comprises the following steps: taking female triploid crassostrea gigas 'Haida No.3' obtained by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas 'Haida No.3' as a female parent; taking a male diploid 'Haida No.3' as a male parent; after the female parent and the male parent are artificially matured, putting ova into seawater to be matured, and when the ova are round through microscopic examination, the ova are matured; conducting insemination on the cured ova and sperms, starting timing after the sperms and the ova are mixed, treating fertilized ova with cytochalasin B, inhibiting discharging of the first polar bodies of the fertilized ova, and collecting and soaking the fertilized ova; and transferring the fertilized ova into a cultivation container for incubation and larva cultivation. The triploid female parent shellfish is obtained by inhibiting the discharge of the first polar bodies of the fertilized ova of the diploid crassostrea gigas 'Haida No.3', and the tetraploid obtained by the triploid female parent shellfish has higher growth advantage due to higher gene heterozygosity.

The invention relates to marine concrete ecological engineering, particularly to a method for constructing an ecological high-durability concrete structure in a marine environment, and belongs to thefield of marine ecological engineering. The construction method comprises the following steps: (1) investigating the sea area of a construction position of a concrete structure; (2) culturing oyster eyelid larvae; (3) constructing the concrete structure; (4) carrying out field attachment; and (5) monitoring larva attachment and management. According to the invention, a novel anti-corrosion technology of marine fixation organisms is adopted, so that the durability of reinforced concrete can be greatly improved, the ecological environment of the sea area is improved, the current situation that the ecological environment is damaged by constructing concrete engineering is changed, and the damaged ecological environment can be repaired.

The invention belongs to the field of fish disease prevention and control and in particular relates to a method for controlling cryptocaryon irritans diseases of fishes by using ozone. According to the method, by virtue of the characteristic that larvae of cryptocaryon irritans are centrally incubated at night, ozone is introduced into a breeding water body in a larva incubation period, and the ozone concentration is kept within a lowest concentration range which is capable of effectively deactivating the larvae of the cryptocaryon irritans; and by cutting off the life history of the cryptocaryon irritans, the purposes of treating and preventing the cryptocaryon irritans diseases can be achieved. By adopting the method, experiments that the larvae of the cryptocaryon irritans are treated with seawater of different ozone concentrations show that the lowest effective deactivation concentration is 0.1-0.15mg / L; and trachinotus ovatus is adopted for effect verification experiments, resultsshow that 411.4+14.7 cysts are generated by each fish on average when the ozone concentration is 0mg / L, 0.4+ / -0.1 cyst is generated by each fish on average when the ozone concentration is 0.1mg / L, and 0 cyst is generated by each fish on average when the ozone concentration is 0.15mg / L. Results show that when the ozone concentration is 0.1-0.15mg / L, the larva infection capability of the cryptocaryon irritans can be remarkably reduced (P is less than 0.05), and the cryptocaryon irritans diseases can be effectively controlled.

The invention relates to an ecological breakwater technology, in particular to a waste concrete marine ecological engineering construction method, and belongs to the field of marine ecological engineering. The method comprises the steps that (1) the sea area where a breakwater is built is investigated; (2) a concrete attachment substrate is prepared; (3) seed oysters are collected quantitatively and farmed; (4) the surface of a waste concrete block is treated; (5) the waste concrete block is placed; (6) the oyster attachment substrate is placed on site; and (7) larva attachment monitoring andmanagement are performed. According to the method, blocks of building waste generated by buildings, infrastructures, etc. are utilized to build marine ecological breakwaters, the waste concrete can befully utilized in accordance with the characteristics of recycling and energy saving of the waste, the oyster attachment substrate can be further induced to grow, the water body is purified, shore-line erosion is prevented, biodiversity is maintained, carbon sequestration is achieved, ocean acidification is slowed down, and an ecological restoration ability is achieved.

The invention discloses an urechis unicinctus fry breeding method. The method comprises the following steps: 1, preparing urechis unicinctus fry; 2, transporting the urechis unicinctus fry; 3, insemination; 4, splashing fertilized eggs into a hatching pond for hatching; 5, breeding planktonic larvae; 6, when the larvae grow to the later period of body section larvae, namely 10-12 body sections of the larvae grow, the larvae are moved into a young urechis rearing pond, meanwhile, the larvae are fed with rhodotorula benthica, and after the larvae enter sea mud and are metamorphosed into wormlike larvae from the body section larvae, the larvae are dragged through a bolting-silk net or collected through drainage; and 7, the young urechis unicinctus is bred for 30-40 days and taken out of the pond when the body length is 5 + / -1 mm. According to the method, rhodotorula benthica is used as bait, digestion of larvae is facilitated, growth is promoted, and manpower and material resources for building a bait culture room and bait culture are omitted; and sea mud is used as a substratum, production management is facilitated, the metamorphosis survival rate of an important link of larva growth can be effectively increased, and the metamorphosis survival rate in the prior art is increased to 70%-75% from 10% of the metamorphosis survival rate in the original art.

The invention discloses a method for breeding perinereis aibuhitensis by using a shrimp pond. The method comprises the following steps of building a breeding facility, breeding perinereis aibuhitensisseedlings, and releasing the perinereis aibuhitensis seedlings. A breeding net cage is used for isolating nereis eggs and planktonic larvae from harmful organisms, so that the nereis eggs and the planktonic larvae are prevented from being predatory; and fertilized eggs are in a suspended state through an oxygenation system and a water inlet system, sufficient dissolved oxygen is provided, the hatching rate of the fertilized eggs is increased, meanwhile, the water inlet system can bring rich unicellular algae bait to the planktonic larvae, and the breeding survival rate of nereis seedlings isincreased.

The invention discloses a molecular monitoring method for long-spiny starfish larvae in a coral reef area. The monitoring method comprises the following steps of 1) manufacturing a DNA template of standard reference long-spiny starfish larvae; 2) filtering and collecting zooplankton samples in the coral reef area; 3) extracting DNA of the zooplankton samples collected in the coral reef area; 4) carrying out PCR amplification and detection on the zooplankton samples. In the PCR detection, a pair of specific primers for the PCR detection of the long-spiny starfish are adopted. A forward primer is AcanP-TF:GCACCGATTTGTCTCTGCCAA. A reverse primer is AcanP-TR:AGTCCTTCTCTCCAGGT. If a target band exists at 785bp, the long-spiny starfish larvae is positive, and if the target band does not exist at the 785bp, the long-spiny starfish larvae is negative. According to the method, the long-spiny starfish larvae can be monitored, and the risk of outbreak of the population of the long-spiny starfish can be pre-warned in advance, so that the aim of early and timely prevention and control is fulfilled.

The invention discloses a method for cultivating burying habitat type shellfish larvae. The method comprises the following steps of after shellfish grows to D-shaped larvae, washing young shellfishesinto a cultivation basket, not adding a substrate into the cultivation basket, ensuring that the pond distribution density in the cultivation basket is 10ind / ml, and adhering a 300-mesh bolting silk net to the inner surface of the cultivation basket; putting the cultivation basket into a cultivation water body for cultivation, feeding bait organisms into the cultivation basket at regular time, andmaintaining the concentration of the bait organisms at 5*10<5>ind / ml; introducing oxygen into the cultivation water body to keep the cultivation water body rich in oxygen; and performing regular young shellfish washing and water changing. Compared with the prior art, the method has the following advantages that 1, the cultivation basket is arranged in the cultivation water body, the cultivation environment is improved, and the young shellfish growth speed and survival rate are increased; 2, the substrate does not need to be added, seawater permeates through the 300-mesh bolting-silk net, andthe influence of zooplankton on young shellfishes is reduced; and 3, the young shellfishes are basically not damaged by washing the young shellfishes and changing water, and the labor cost is greatlyreduced.

The invention provides an efficient outdoor ecological pond artificial fry breeding method for lutjanus argentimaculatus. The method comprises the following steps: (1) arranging a pond, and laying volcanic rock, gravel, vermiculite and biological ceramsite on the bottom of the pond; (2) before larva fish eats food, inoculating 60,000-90,000 / mL of thalassiosira, 40,000-60,000 / mL of chlorella and 10,000-30,000 / mL of golden algae into the pond, and after the water quality in the pond is stable, placing the lutjanus argentimaculatus into the pond, wherein the placing density is 20,000-40,000 / mu; (3) after the larva fish eats food, in the larva fish period, feeding the larva fish with oyster larvae every day, and splashing soybean milk in the pond, wherein the density of the oyster larvae is 5-8 / mL; (4) in the juvenile fish period, feeding juvenile fish with rotifers and Moina mongolica daday, wherein the density of the rotifers is 3-5 / mL, the density of the Moina mongolica daday is 50-80 / L, and meanwhile, splashing soybean milk and bean dregs in the pond at the same time; and (5) in the juvenile fish period, feeding the juvenile fish with the rotifers and heliozooid, wherein the density of the rotifers is 8-10 / mL, the density of the heliozooid is 10-25 / mL, and harvesting the lutjanus argentimaculatus after keeping breeding for 20-40 days. The survival rate of harvested lutjanus argentimaculatus fries is high, the specification is large, and variation is reduced.

The invention provides a breeding method of a stress-resistant crassostrea gigas new variety. The method comprises the steps that 1, crassostrea gigas with regular shell shapes is selected, and elimination selection is carried out; 2, parent shellfishes with the strongest stress resistance are selected out, and dissecting is carried out after artificial maturation promotion to obtain sperms and ova; 3, after being cleaned, the ova are put into seawater filtered by sand to be cured; 4, one-to-one fertilization is carried out on the cured ova and sperms in sand-filtered seawater to establish families; and 5, the fertilized ova of the different families after fertilization are put into different plastic barrels, the fertilized ova are incubated in sand-filtered seawater, and larva cultivationis carried out. According to the method, along with prolonging of oyster culture time and year-by-year increasing of the oyster death rate, the advantages of the new variety are more remarkable, thelower death rate enables farmers to try to culture oysters of two years old or above, large-size and high-quality oysters more popular with consumers, especially e-commerce, can be produced, and the method has great popularization value.

The invention discloses a method for improving adhesion of coral larvae. The coral larvae are placed in seawater containing calcium ions to be cultivated, and metamorphosis and adhesion of the coral larvae are promoted. By means of the method, the adhesion rate of the coral larvae can be remarkably increased, and the time needed by larva attachment is shortened. The adhesion rate of the larvae can reach 79.2 + / -5.56% within 24 h while the survival rate of the coral larvae is ensured to be close to 100%. In comparison research with crustose coralline algae, the survival rate and the adhesion rate of the coral larvae treated by the method are higher than those of the coral larvae treated by the crustose coralline algae under the same treatment. And after 24 hours of treatment with calcium chloride, the growth ability of the subsequent coral larvae is not affected. Meanwhile, under the condition that the survival and adhesion rate of the coral larvae are guaranteed, calcium ions are used for inducing the coral larvae to adhere efficiently and stably, and calcium chloride is more convenient and quicker to add in an experiment, is simple to operate and is better controlled as a single variable. Therefore, the method also has the potential of large-scale engineering application.

The present invention relates to an octopus minor-specific octopressin peptide comprising the amino acid sequence of SEQ ID NO: 3, and the peptide, by promoting the brooding behaviors of mother octopus minor to protect her eggs, may increase the hatched larva rate when applied to partial cultivation of octopuses, and thus may be used for the growth of octopus resources.

The invention discloses an artificial breeding method of sea cauda. The method comprises the steps of parent collection and maturity promotion, spawning induction and incubation, larva cultivation, juvenile mollusk cultivation and the like. The method has the advantages and positive effects that a long-term unsuccessful rearing technology of the clam offspring seeds is broken through; a set of method suitable for artificial breeding of the sea cauda is established, a large number of sea cauda fries are bred, and the domestic blank is filled. The method disclosed by the invention has important practical significance on culture development, resource recovery and biological diversity maintenance of the sea cauda.

The invention provides an artificial breeding method for large-size young clams, which belongs to the technical field of shellfish breeding and comprises the steps of juvenile clam breeding and middle-sized clam breeding. By adopting the method provided by the invention, large-scale breeding of the clams can be realized, the induced spawning discharge rate of the parent clams of the clams is 60%, the fertilization rate is 85%, the hatching rate is 90%, and the larva metamorphosis rate is 50% or above; the survival rate reaches 30% or above, and when shells of the clams grow to 2-3 cm, the clams can be moved to a natural sea area to be cultured.

The invention discloses a three-stage cultivation method for large-scale seedlings of meretrix meretrix suitable for the cold sea area in northern my country. The first stage: screening of clam broodstock, oviposition, hatching of fertilized eggs, indoor artificial cultivation of planktonic larvae, and abnormal attachment of shell top larvae and so on, the shell length is about 0.9mm, and this stage is from the end of June to the middle of October; the second stage: the process of overwintering the clam spat in an outdoor pond until the shell length is about 4.5mm, and the time is from From mid-October to April of the second year; the third stage: the process of cultivating the overwintered meretrix meretrix spat through the middle of an outdoor pond until the shell length is more than 1.0 cm, and the time course is from April to July. The method can provide a technical guarantee for the large-scale production of clam seed, and provide sufficient seed supply for the development of the clam breeding industry.