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Method for improving adhesion of coral larvae

A technology for corals and larvae, applied in the field of coral breeding, can solve the problems of attachment to algae and the influence of environmental factors, and achieve the effects of shortening the required time, improving the attachment rate and simple operation

Active Publication Date: 2021-06-11
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technique of shell coralline algae to induce coral larvae attachment has cases where the larvae attach to the algae and may be affected by other environmental factors

Method used

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  • Method for improving adhesion of coral larvae
  • Method for improving adhesion of coral larvae
  • Method for improving adhesion of coral larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Determining calcium ions have the ability to induce coral larvae attachment

[0023] In September 2018, the antlers cup-shaped coral from Sanya City, Hainan Province, was collected for experimental subjects, relying on the Hainan Tropical Marine Biological Experimental Station, South China Sea Institute, China Academy of Sciences.

[0024] The collected antler cup coral larvae is placed in a six-hole plate in natural sea water. Use natural seawater to configure different concentrations of sodium chloride, calcium chloride, calcium sulfate solution. Then, in the natural seawater in the six-hole plate, that is, the final concentration of 10 mm, 40 mm, 80 mm, 10 mm, calcium chloride, calcium sulfate and calcium carbonate solution, respectively, on the basis of natural seawater, 10 mm and 40 mM. figure 1 ), In the culture temperature of 28 ± 0.5 ° C, 12-12h, light and dark circulation, light 200 μmol photons m -2 s -1 Under the conditions, cultured coral larvae, after...

Embodiment 2

[0025] Example 2 Determined calcium chloride optimal induction concentration

[0026] In September 2018, the antlers cup-shaped coral from Sanya City, Hainan Province, was collected for experimental subjects, relying on the Hainan Tropical Marine Biological Experimental Station, South China Sea Institute, China Academy of Sciences.

[0027] The collected antler cup coral larvae is placed in a six-hole plate in natural sea water. Use natural seawater to configure a specific concentration of calcium chloride solution. In the natural sea water in the six-hole plate, that is, the final concentration is 20-80 mm based on the natural sea water, and the final concentration is 20-80 mm, and the culture temperature is 28 ± 0.5 ° C, 12-12h light dark cycle, and the light 200 μmol photons m -2 s -1 Coral larvae was cultured under conditions, and 7D was allowed to culture 7d and the survival rate and admission rate were observed, each treatment 6 repetitions. This example continuously process...

Embodiment 3

[0028] Example 3 Exploring the induction capabilities of calcium ions and shell coral locus for 24h

[0029] In August 2019, the antlers cup-shaped coral from Sanya City, Hainan Province, was collected for experimental subjects, relying on the Hainan Tropical Marine Biological Experimental Station of the South China Sea Institute of China.

[0030] The collected antler cup coral larvae is placed in a six-hole plate in natural sea water. The experimental group was added to the natural seawater in the six-well plate (final concentration), and the control group was placed in a control group to add 40 mm sodium chloride and shell-shaped coral algae, at the culture temperature of 28 ± 0.5 ° C, 12-12H. Dark loop, light 200μmol photons m -2 s -1 Under the conditions, the coral larvae attached under treatment was followed, and the process of cultivation did not change water, not inflated, and each treatment was 4 repetitions. Take the shell coral algae used by the experiment, collect self...

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Abstract

The invention discloses a method for improving adhesion of coral larvae. The coral larvae are placed in seawater containing calcium ions to be cultivated, and metamorphosis and adhesion of the coral larvae are promoted. By means of the method, the adhesion rate of the coral larvae can be remarkably increased, and the time needed by larva attachment is shortened. The adhesion rate of the larvae can reach 79.2 + / -5.56% within 24 h while the survival rate of the coral larvae is ensured to be close to 100%. In comparison research with crustose coralline algae, the survival rate and the adhesion rate of the coral larvae treated by the method are higher than those of the coral larvae treated by the crustose coralline algae under the same treatment. And after 24 hours of treatment with calcium chloride, the growth ability of the subsequent coral larvae is not affected. Meanwhile, under the condition that the survival and adhesion rate of the coral larvae are guaranteed, calcium ions are used for inducing the coral larvae to adhere efficiently and stably, and calcium chloride is more convenient and quicker to add in an experiment, is simple to operate and is better controlled as a single variable. Therefore, the method also has the potential of large-scale engineering application.

Description

Technical field: [0001] The present invention belongs to the field of coral breeding, and in particular to a method of increasing coral larvae. Background technique: [0002] The coral reef ecosystem contains rich ecological service functions, and they have high primary productive forces, providing food for more than 1 / 4 marine organism and is closely related to human social resources, economy, culture. The coral reef is gradually degraded under the influence of global climate and human behavior. Reef coral acts as a key role in coral reef, which built a three-dimensional structure of a coral reef. In coral reef artificial repair, sexual reproduction and hazardous reproduction are considered to be the main supplementary strategy. Severe breeding is a major means of reserve coral breeding, which can effectively expand population, low cost, and strong practicality. However, a wide range of applications of hazybly may reduce the genetic diversity of corals. Instead, coral breeding n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 杨清松张文谦董俊德凌娟唐小玉龙丽娟殷建平张燕英李洁张偲
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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