39 results about "High pressure freezing" patented technology

A specimen holder for a high-pressure freezing device comprises a first shaped part (2) and a second shaped part (3) that form, in the mutually joined state, a specimen space (6). The first shaped part (2) is embodied in a cup shape, and possesses a bottom (5) that closes off the specimen chamber (6) toward the outside to prevent contact between the specimen and a pressure medium.

An automatic loading apparatus (12) for specimens (1) for high-pressure cryosubstitution is disclosed. The automatic loading apparatus (12) is connected to a high-pressure freezing device (40). Specimens (1) are inserted in a holder (2). By means of a slider (4), the specimens (1) and holders (2) are conveyed to the automatic loading apparatus (12). The guidance element (26) allows exact positioning of the holder (2) in the clamping element (14). The automatic loading apparatus makes possible rapid transfer of the holder (2) having the specimen (1) from, for example, an optical microscope to the high-pressure freezing device (40).

Methods of sterilizing a temperature sensitive material, such as an acidified or non-acidified food product, pharmaceutical product or cosmetic product, are disclosed. The methods comprise freezing the temperature sensitive material to an initial temperature of less than or equal to −2° C. and then either (i) pressurizing the frozen temperature sensitive material to a first elevated pressure of at least 250 MPa for a predetermined first period of time of at least 3 minutes or (ii) pressurizing the frozen temperature sensitive material to a first elevated pressure of at least 250 MPa for a predetermined first period of time of at least 90 seconds, releasing the first elevated pressure for a predetermined pause period of time and then pressurizing the temperature sensitive material to a second elevated pressure of at least 250 MPa for a predetermined second period of time of at least 90 seconds.

A modular specimen holder (10′) for high pressure freezing and / or X-ray crystallography of a specimen has a specimen holding element (100′) and an extension element (200′) connectable with and separable from each other; the specimen holding element (100′) including a tubule (120′) adapted to hold the specimen and a base element (110′) adapted to hold the tubule (120′), wherein a distance from a bottom of the base element (110′) to a top of the tubule (120′) is a first distance (d1); the extension element (200′) being connectable with the base element (110′), wherein, when the extension element (200′) and the base element (110′) are connected with each other, a second distance (d2) from a bottom of the extension element (200′) to the top of the tubule (120′) is larger than the first distance (d1).

The present invention discloses a cryosurgery device capable of achieving freezing and rewarming by adjusting gas pressure. The cryosurgery device comprises a high-pressure gas source, a high-pressure pressure-limiting valve, a freezing gas main pipe and a freezer for throttling and freezing; high-pressure gas divided from a high-pressure gas pipe passes through a shunting electromagnetic valve and a low-pressure pressure-limiting valve for pressure limiting, and is transmitted to the freezer via a heating gas main pipe for shunting and rewarming; gas from a low-pressure gas source passes through a low-pressure electromagnetic valve and the low-pressure pressure-limiting valve for pressure limiting, and is transmitted to the freezer via the heating gas main pipe for low-pressure rewarming; and the heating gas main pipe is provided with a gas heater capable of preheating low-pressure rewarming gas and achieving a controllable heating function. The technical prejudice that throttling refrigeration type gas can only be used for freezing is corrected, warming is performed by utilization of residual gas of high-pressure freezing gas and low-pressure gas, the rewarming problem and low-temperature working medium waste problem of a gas throttling type cryosurgery device are solved, and freezing, rewarming and heating functions are achieved.

A specimen holder for a high-pressure freezing device comprises a first shaped part (2) and a second shaped part (3) that form, in the mutually joined state, a specimen space (6). The first shaped part (2) is embodied in a cup shape, and possesses a bottom (5) that closes off the specimen chamber (6) toward the outside to prevent contact between the specimen and a pressure medium.

The invention discloses a pressure-adjustable mine high- and low-pressure water energy exchange system. The pressure-adjustable mine high- and low-pressure water energy exchange system comprises an underground integrated control center system of a monitoring system, which comprises an industrial control computer and is connected with a hydraulic station and a three-cavity water energy exchange device. While high-pressure freezing water is subjected to pressure relief, hot water is conveyed to the ground surface from the underground part through a water energy conversion pipe, so that the potential energy exchange between the cold water and the hot water is realized; the opening degree of an electric flow adjusting valve is controlled through signals of the temperature and the flow acquired by a temperature sensor and a flow sensor, and the flow of the cold water is adjusted to be matched with end load; an underground circulating water treatment unit guarantees the backwater quality of the hot water, makes sure the quality of the water flowing through the valve, and prolongs the service life of the valve; a variable frequency water complementing system guarantees the system to stably run; and the hydraulic station is used for controlling the valve to open and close according to a certain logic sequence through an instruction of the industrial control computer, guaranteeing the system to continuously and stably run, and monitoring the status of the system equipment.

The present invention relates to a pretreatment method for harmful substances in milk powder samples, in particular to a pretreatment method for smelting and extracting harmful substances in milk samples in a frozen region, mainly used for chloramphenicol residues in formula milk powder for infants and young children The method for measuring belongs to the field of food processing detection. The method comprises the following steps: (1) taking a solid milk powder sample, adding ultrapure water, or weighing an emulsion sample, mixing it with an organic extraction reagent, first extracting, then shaking to obtain a treatment solution; (2) pouring the treatment solution (3) Put the high-pressure freezing extraction kettle into the refrigerator to freeze, or put it in an ice bath; (4) Take out the high-pressure freezing extraction kettle, absorb the upper organic phase, and filter to obtain the filtrate. The pretreatment method for extracting harmful substances in milk samples by smelting in a frozen region provided by the present invention not only has high extraction efficiency, can reduce or even eliminate interfering components, but also is simple and convenient to operate, and is helpful for effective detection of harmful substances in milk samples.

The invention discloses a gas throttle type cryosurgical device and a method for controlling the same. A plurality of rewarming processes can be implemented by the aid of the gas throttle type cryosurgical device and the method. Rewarming (shunt rewarming) effects can be realized by the aid of gas which is shunted from high-pressure freezing gas and has limited pressures lower than throttle working pressures; rewarming (residual gas rewarming) effects can be realized by the aid of residual gas with the pressures lower than freezing working pressures; rewarming (normal-pressure rewarming) effects can be realized by the aid of normal-pressure gas; rewarming (throttle rewarming) effects can be realized by the aid of throttle pyrogenic gas; rewarming (exhaust rewarming) effects can be realized by the aid of gas source shutting and equipment exhaust procedures. The gas throttle type cryosurgical device and the method have the advantages that the gas throttle type cryosurgical device is also provided with gas preheating devices, accordingly, the rewarming efficiency can be improved, the rewarming temperatures can be increased, and the gas throttle type cryosurgical device has a freezing function, a rewarming function and a heating function; the technical prejudice that throttle frigorific gas only can be used for freezing can be corrected, rewarming difficult problems of existing gas throttle type cryosurgical equipment can be solved, the gas utilization rate can be increased, the surgical cost can be reduced, energy can be saved, environments can be protected, the rewarming performance can be enhanced, the gas throttle type cryosurgical device has an additional thermotherapy function, and the gas throttle type cryosurgical device and the method are beneficial to promoting popularization and improvement of cryoablation technologies.

The invention belongs to the field of food processing and microbial fermentation, and in particular relates to a method for improving the tenderness of goose meat based on multiple fermentations, comprising the following steps: S1: subjecting the goose meat to ultra-high pressure freezing treatment; The tenderizer was injected into the meat; S3: the goose meat treated in S2 was tumbled, marinated and tenderized in vacuum; S4: the goose meat treated by Streptococcus thermophilus was used for primary fermentation and secondary fermentation respectively; S5: S4 The treated goose meat is stretched and compressed; S6: the goose meat treated in S5 is cooked at 95 to 100° C. for 10 to 14 minutes. The method of the invention can improve the tenderness of the cooked goose meat, is tender and juicy without affecting the original flavor of the goose meat, reduces the cooking loss rate, and effectively solves the problem of the goose meat becoming hard.