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Pretreatment method for smelting and extracting harmful substances in milk sample in freezing region

A technology for hazardous substances and regional smelting, which is applied to the pretreatment of hazardous substances in smelted and extracted milk samples in freezing areas, and the pretreatment of hazardous substances in milk powder samples, which can solve the problems of secondary pollution, high consumption, and increased analysis costs. , to achieve the effect of eliminating interfering components, simple and convenient operation, and high extraction efficiency

Active Publication Date: 2015-06-24
WISSUN INT NUTRITION GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these pretreatment methods often need to consume a large amount of organic solvents, corresponding extraction materials and corresponding pretreatment instruments, which not only increase the analysis cost, but also cause secondary pollution

Method used

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  • Pretreatment method for smelting and extracting harmful substances in milk sample in freezing region
  • Pretreatment method for smelting and extracting harmful substances in milk sample in freezing region
  • Pretreatment method for smelting and extracting harmful substances in milk sample in freezing region

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0056] (1) Weigh 8g of solid milk powder sample, add 65mL of ultrapure water to the solid milk powder sample, mix with 35mL of saturated ethyl acetate, vortex extract for 3min, and shake at medium speed for 3min to obtain the treatment solution;

[0057] (2) Pour the treatment liquid obtained in step (1) into a high-pressure freezing extraction kettle, cover the lid tightly, and tighten the lid of the kettle body, then open the sealing bolt on the lid of the kettle, and use a syringe to pass through the small hole in the lid of the kettle Inject an appropriate amount of saturated ethyl acetate into the high-pressure freezing extraction kettle, and discharge the remaining air bubbles in the high-pressure freezing extraction kettle;

[0058] (3) Screw the sealing bolt on the lid of the kettle into the lid of the kettle, and tighten it, put the high-pressure freezing extraction kettle in a refrigerator at -20°C for 2 hours, or put it in an ice bath for 30 minutes;

[0059] (4) Ta...

Embodiment 1-2

[0061] (1) Weigh 5 g of the solid milk powder sample, add 50 mL of ultrapure water to the solid milk powder sample, mix with 20 mL of saturated ethyl acetate, vortex extract for 1 min, and then shake at a medium speed for 2 min to obtain the treatment solution;

[0062] (2) Pour the treatment liquid obtained in step (1) into a high-pressure freezing extraction kettle, cover the lid tightly, and tighten the lid of the kettle body, then open the sealing bolt on the lid of the kettle, and use a syringe to pass through the small hole in the lid of the kettle Inject an appropriate amount of saturated ethyl acetate into the high-pressure freezing extraction kettle, and discharge the remaining air bubbles in the high-pressure freezing extraction kettle;

[0063] (3) Screw the sealing bolt on the lid of the kettle into the lid of the kettle, and tighten it, put the high-pressure frozen extraction kettle in a refrigerator at -18°C for 1.8 hours, or put it in an ice bath for 25 minutes; ...

Embodiment 1-3

[0066] (1) Weigh 10 g of solid milk powder sample, add 80 mL of ultrapure water to the solid milk powder sample, mix with 50 mL of saturated ethyl acetate, vortex extract for 5 minutes, and then oscillate at medium speed for 5 minutes to obtain the treatment solution;

[0067] (2) Pour the treatment liquid obtained in step (1) into a high-pressure freezing extraction kettle, cover the lid tightly, and tighten the lid of the kettle body, then open the sealing bolt on the lid of the kettle, and use a syringe to pass through the small hole in the lid of the kettle Inject an appropriate amount of saturated ethyl acetate into the high-pressure freezing extraction kettle, and discharge the remaining air bubbles in the high-pressure freezing extraction kettle;

[0068] (3) Screw the sealing bolt on the lid of the kettle into the lid of the kettle, and tighten it, put the high-pressure freezing extraction kettle in a refrigerator at -22°C for 2.2 hours, or put it in an ice bath for 35 ...

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Abstract

The invention relates to a pretreatment method for smelting and extracting harmful substances in a milk sample in a freezing region, and in particular relates to a pretreatment method for smelting and extracting harmful substances in the milk sample in the freezing region. The pretreatment method is mainly used for measuring residues of choramphenicols in infant formula milk powder, and belongs to the field of food processing and detection. The method comprises the following steps: (1) weighing a solid milk powder sample and adding ultra-pure water or weighing the milk powder sample, mixing the milk powder sample with an organic extraction reagent, and performing extraction and oscillation in sequence to obtain treatment liquid; (2) pouring the treatment liquid into a high-pressure freezing extraction kettle, and injecting ethyl acetate; (3) putting the high-pressure freezing extraction kettle into a refrigerator for freezing or into an ice bath; and (4) taking out the high-pressure freezing extraction kettle, sucking an organic phase on the upper layer, and filtering to obtain filtrate. The pretreatment method for smelting and extracting the harmful substances in the milk sample in the freezing region is high in extraction efficiency; interference components can be reduced and even eliminated; furthermore, the method is easy and convenient to operate, and effective detection on the harmful substances in the milk sample is facilitated.

Description

technical field [0001] The present invention relates to a pretreatment method for harmful substances in milk powder samples, in particular to a pretreatment method for smelting and extracting harmful substances in milk samples in a frozen region, mainly used for chloramphenicol residues in formula milk powder for infants and young children The method for measuring belongs to the field of food processing detection. Background technique [0002] Chloramphenicol (CAP) is a high-efficiency broad-spectrum antibiotic, commonly used in the treatment of various infectious diseases in animals, and has a strong inhibitory effect on a variety of pathogenic bacteria. Due to the low price and strong disease resistance of chloramphenicol, illegal administration of chloramphenicol to dairy cows occurs from time to time, resulting in chloramphenicol residues in milk powder, which brings potential harm to the health of infants and young children. At present, the European Union and the Unite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N30/06
Inventor 陈光耀林玉宙池松炎
Owner WISSUN INT NUTRITION GRP
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