Cadmium contaminated soil restoration method
A cadmium-contaminated soil and soil technology, applied in the field of soil heavy metal pollution control, can solve the problem of low repair efficiency, achieve the effect of improving enrichment capacity, increasing absorption, improving transferability and availability
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Embodiment 1
[0025] 1. Soil for the test: The soil was taken from the 0-20cm topsoil in the area around a lead-zinc mine in Nanjing. The soil was air-dried, ground, and sieved (20 mesh) before use. The content of Cd in the polluted soil is 8.21 mg·kg, more than 8 times higher than the national third-level standard -1 , pH 7.10.
[0026] 2. Preparation of black nightshade seedlings: sterilize the black nightshade seeds with 0.5% NaClO solution for 20 minutes, rinse them with distilled water, rinse off the shriveled grains, and let them dry naturally for later use. Then spread the sterilized vermiculite in the tray with a thickness of 4-6cm, and sow nightshade seeds therein. The vermiculite trays filled with nightshade seeds are placed in the incubator, and the trays are put into the cultivation room after the nightshade seeds germinate. The cultivation room uses fluorescent tubes to simulate natural light, the day and night cycle is 16 hours of light, 8 hours of darkness, and the relative...
Embodiment 2
[0032] Embodiment 2 applies the influence of polyaspartic acid and microbial bacterial agent to Cd content of Solanum nigrum jointly
[0033] The experimental method is the same as in Example 1, and the results are shown in Table 2. The combined application of microbial agents and polyaspartic acid can significantly increase the Cd content in Solanum nigrum, and the effect is: PASP+PGPR>PASP>PGPR. Compared with the single application of PGPR or PASP, the combined application increased the Cd content in the shoot and underground parts of Solanum nigrum by 252.1%-344.1% and 131.3%-312.6%, respectively. It can be concluded from Table 3 that the combined application of microbial agents and polyaspartic acid has increased the extraction of Cd in the shoots and underground parts of Solanum nigrum up to 8 times, and the significance is higher than that of a single application, indicating that the use of polyaspartic acid The combination of acid and microorganisms to assist Solanum ni...
Embodiment 3
[0039] A method for repairing cadmium-contaminated soil, comprising the following steps:
[0040](1) Cultivate Solanum nigrum seedlings, and when the seedlings grow to three leaves and one heart, select the robust and consistent seedlings to be transplanted into the cadmium-contaminated soil that needs to be repaired;
[0041] The specific process of cultivating black nightshade seedlings is as follows: sterilize black nightshade seeds with 0.5% NaClO solution for 20 minutes, rinse them with distilled water, rinse off the shriveled grains, and dry them naturally for later use; 4-6cm, where the nightshade seeds are sown; the vermiculite trays containing the nightshade seeds are placed in the incubator, and the trays are placed in the cultivation room after the nightshade seeds germinate; the cultivation room uses fluorescent tubes to simulate natural light, and the day and night cycle is 16h Light, 8h dark, relative humidity controlled at 80%.
[0042] (2) Solanum nigrum seedl...
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