Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of samples for high pressure freezing

A high-pressure, sample technology, applied in the preparation of test samples, household refrigeration equipment, applications, etc., can solve time-consuming and complicated problems

Pending Publication Date: 2022-03-22
LEICA MICROSYSTEMS GMBH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Transfer to these cassettes is complicated and time-consuming, especially when samples were previously housed in the mentioned culture chambers
This is a major obstacle to rapid fixation of cell states in prior art methods and arrangements

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of samples for high pressure freezing
  • Preparation of samples for high pressure freezing
  • Preparation of samples for high pressure freezing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0062] figure 1 A culture chamber 900 for culturing a sample, which does not form part of the invention, is shown in a simplified cross-sectional view and has been described at the outset. Please refer to the instructions above. It should also be noted that the present invention is not intended to be limited to the particular type of culture chamber shown here.

[0063] figure 2 Shown in exploded view is a high pressure freezer box 30, the sample holding means, which may be used with the present invention. Cassette 30 may be used with the above-mentioned high pressure freezers known in the art. For example, reference is made to the literature on the "EM ICE", "EM HPM100" and "EM PACT" systems of Leica Microsystems cited above (e.g. reference to DE 10 2013 003 164 A1 which further describes details of high pressure freezing systems) and to the scientific review literature, For example Kaech & Ziegler (see above). In the assembled state, the cassette 30 can be transferred ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a device (10) for preparing a microscope sample (1) for a high-pressure freezing process, in which the sample (1) is provided using an arrangement of an intermediate plate (33) comprising a high-pressure freezing cartridge (30) and a culture chamber (100, 200), the intermediate plate (33) being attached to the culture chamber (100, 200) and being detachable from the culture chamber (100, 200) by effecting a relative movement between the intermediate plate (33) and the culture chamber (100, 200), the culture chamber (100, 200) is provided with an opening (36), and the sample (1) is provided on an encapsulation element (37) to be fitted into the opening (36) of the intermediate plate (33), in which the device (10) comprises an engagement structure (11, 21) adapted to engage with the intermediate plate (33) and restrict movement of the intermediate plate (33) when the culture chamber (100, 200) is moved in order to effect said relative movement between the intermediate plate (33) and the culture chamber (100, 200), thereby detaching the intermediate plate (33) from the culture chamber (100, 200). Furthermore, a culture chamber (100, 200), an intermediate plate (33) of a high-pressure freezing box (30), an arrangement comprising these components and a corresponding method are also part of the invention.

Description

technical field [0001] The invention relates to a device for preparing microscopic samples for high-pressure freezing processes as well as means and corresponding methods that can be used in conjunction with such a device. Background technique [0002] During light microscopy imaging, living cells should be maintained under favorable environmental conditions. Stage incubators, stage heaters, carbon dioxide chambers, etc. are common tools used for this purpose. [0003] There are several stage incubators and chambers for conditioning the gas atmosphere around samples during light microscopy examinations available. The operating principle of a particular type of culture chamber is disclosed in DE 20 2016 007 488 U1, which offers the option of transferring samples between different locations while still maintaining ambient conditions. [0004] under the figure 1 , the components of this type of culture chamber are shown in longitudinal section. The present invention is not ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/42
CPCG01N1/42C12M45/20C12M41/36C12M41/14F25D3/10F25D2400/30
Inventor 赖讷·沃格利施保罗·武尔青格
Owner LEICA MICROSYSTEMS GMBH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com