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36 results about "EGFR L858R" patented technology

DdPCR detection method for lung cancers EGFR L858R and 19Del and application of ddPCR detection method

InactiveCN107663533AReliable Analysis BasisNumber of optimization cyclesMicrobiological testing/measurementPurification methodsTreatment effect
The invention relates to the technical field of medicines and biology and particularly relates to a ddPCR detection method for lung cancers EGFR L858R and 19Del and application of the ddPCR detectionmethod. The detection method comprises the steps of separating hemocyte and plasma of peripheral blood, carrying out cfDNA extraction by virtue of a two-step purification method, respectively designing two probes and one pair of primers aiming at lung cancer-relevant EGFR L858R loci and EGFR 19# exon deletion loci, carrying out ddPCR detection by taking extracted cfDNA as a template, and finally carrying out data analysis on an obtained amplification product, so as to obtain the proportion of EGFR sensitively-mutated L858R and 19Del. The application comprises the application of the ddPCR detection method in the treatment of tumor patients, medication-relevant genetic locus mutation information is provided for the tumor patients in the treatment process of the terminal lung cancer, and important reference frame is provided for the individualized accurate medication guidance, pesticide effect evolution and treatment effect monitoring of the lung cancers.
Owner:深圳海普洛斯医学检验实验室

Primer probe composition, kit and method for detecting EGFR specific gene mutations through 3D (Three-dimensional) digital PCR (Polymerase Chain Reaction)

The invention discloses a primer probe composition, a kit and a method for detecting EGFR specific gene mutations through 3D (Three-dimensional) digital PCR (Polymerase Chain Reaction). The primer probe composition comprises primers and probes for detecting EGFR gene No.19 to No.21 exon specific mutations, wherein the mutations are EGFR T790M, EGFR L858R, EGFR 2235_2249del15 and EGFR 2236_2250del15. By applying the primer probe composition for detecting the EGFR specific gene mutations through 3D-PCR provided by the invention, four common mutation sites related to lung cancer are included and are respectively detected through the primers and the probes designed according to the different mutation sites, so that the multiple mutations can be detected, the detection efficiency is high, and a result is more intuitive and clearer since the primer probe composition is combined with a 3D-PCR detection method for detecting.
Owner:GENE CRAB BIOTECH CO

Quinolinone-containing pyrimido-five-membered heterocyclic compounds and a preparation method and application thereof

The invention discloses quinolinone-containing pyrimido-five-membered heterocyclic compounds and a preparation method and application thereof and belongs to the field of medicinal chemistry. The compounds are shown in the general formula I. The compounds have significant inhibitory and killing effects on a variety of tumor cells such as H1975, A549, PC9, PC3 and HGC and have good inhibitory activity to a human epidermal growth factor receptor EGFR-L858R / T790M mutant in the in-vitro kinase level. The compounds can be used as candidate or lead compounds in preparation of antitumor drugs.
Owner:ZHENGZHOU UNIV

T790M mutant epidermal growth factor receptor (EGFR) inhibitor and application of same in preparation of antitumor drugs

The invention provides a T790M mutant EGFR inhibitor and an application of the same in preparation of antitumor drugs. The inhibitor is a pyrimidine compound with structural characteristics of a formula (I). The compound can inhibit various tumor cells and particularly can act on EGFR L858R / T790M and EGFR E 745_A750 / T790M lung cancer cells selectively, and the IC 50 of the compound is 10 timers and even 100 times higher than that of wild cancer cells. The compound is the protease inhibitor which is capable of overcoming prior EGFR-TKI drug resistance and has selectivity and can be applied to preparation of antitumor drugs. (img file= 'DDAS0000708455660000011. TIF' wi= '716' he= '576' / ).
Owner:PHARMA SHANGHAI

Kit for detecting 22 mutations of EGFR genes by utilizing digital PCR technology

The invention discloses a kit for detecting 22 mutations of EGFR genes by utilizing a digital PCR technology. The kit comprises at least one of a reagent EGFR 19 del, a reagent EGFR T790M and a reagent EGFR L858R, as well as positive quality control and negative quality control. The kit disclosed by the invention is capable of quantitatively determining L858R point mutation, T790M point mutation and 20 deletion mutations of 19 del of the EGFR genes in samples comprising tissue DNA and free DNA of plasma and urine. The primer, probe and kit disclosed by the invention are high in detection accuracy, high in sensitivity and convenient to operate, and reference is made to TKI type drug choice of the targeted EGFR.
Owner:BEIJING ACCB BIOTECH

Pyrimidotricyclic compounds or pyrimidotetracyclic compounds, pharmaceutical composition and applications thereof

The invention discloses pyrimidotricyclic compounds or pyrimidotetracyclic compounds having a structure represented by the formula (I), (II) or (III), pharmaceutically-acceptable salts, stereoisomers, or prodrug molecules. The compounds can inhibit various tumor cells, and especially, the compounds can selectively act on lung cancer cells of EGFR L858R / T790 and EGFR E745 A750 / T790M. Compared to wild type cancer cells, the IC50 of the compounds is 10 times, 100 times or even 1000 times higher. The compounds are a novel protein kinase inhibitor, which can overcome the drug resistance of EGFR-TKI and has selectivity.
Owner:GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI

Preparation method and application of electrochemical biosensor for detecting NSCLC circulating tumor gene

The invention discloses a preparation method and application of an electrochemical biosensor for detecting an NSCLC circulating tumor gene. The preparation method comprises the following steps of taking a prepared NG-PEI-COF nano composite material as a substrate material of the sensor, and taking Prussian blue generated by Fe-MOF and Fe<2+> as a signal amplification material; the sandwich type biosensor for detecting the circulating tumor gene EGFR L858R is constructed by utilizing nucleic acid hybridization. The method is advantaged in that the biosensor provided by the invention not only has a stronger signal amplification effect, but also has advantages of wide linear range, strong specificity, short analysis time and the like, and can provide a new method for diagnosing the non-small cell lung cancer.
Owner:CHONGQING MEDICAL UNIVERSITY

Method for detecting EGFR L858R locus in lung cancer by ddPCR and application thereof

InactiveCN109762878ALow nucleic acid contentLow Mutation Sequence ContentMicrobiological testing/measurementWild typeBlood plasma
The invention discloses a method for detecting an EGFR L858R locus in lung cancer by ddPCR and an application thereof. According to the sequence of an EGFR gene, a pair of amplified primers includingan upstream primer and a downstream primer are designed; according to the mutation sequence of L858R (2573T>G, COSM6224), a probe for detecting a wild type sequence and a probe for detecting a mutation sequence are designed. The primers and the probes are used for ddPCR and used for detecting and analyzing important loci related to targeted medication for lung cancer in ctDNA. Firstly, DNA is extracted from peripheral plasma of lung cancer patients, and the mutation condition of the mutation locus L858R of EGFR is detected by the ddPCR technology, so as to guide the patients to use drugs. Themethod can be used for diagnosis of early-stage lung cancer such as stage I, II and III A of tumor, can detect 0.0625% EGFR gene L858R mutation, and has the sensitivity reaching 10 times or more thatof conventional methods.
Owner:铭时医疗科技(宁波)有限公司

Method for detecting circulating tumor cells and uses thereof

A method for the detection and / or diagnosis of cancer in a patient is disclosed. The method involves detecting circulating tumor cells (CTCs) in a sample obtained from the patient using an antibody specifically binding to an antigen in a protein expressed by the CTCs, wherein the antigen comprises a mutation and wherein the mutation renders the patient receptive or resistance to an anti-cancer drug. Claimed methods include the use of an antibody specific for the EGFR L858R or EGFR exon 19 (ΔE746-A750) mutations for detecting lung cancers and the use of an antibody specific for the K-Ras G12V, K-Ras G12C, K-Ras G12S, K-Ras G12D, or K-Ras G13D mutations for detecting colorectal cancers.
Owner:AGENCY FOR SCI TECH & RES +1

Novel EGFR and alk dual inhibitor

The present application provides a compound of formula I, which is a EGFR and ALK dual inhibitor and can be used alone or in combination with other therapeutic agents to treat diseases such as non-small cell lung cancer. The compounds of the present application are useful in the treatment of diseases carrying the EGFR wild-type gene, or carrying the EGFR T790M mutant gene and / or the EGFR L858R mutant gene and / or the EGFR delE746_A750 mutant gene, or in the treatment of diseases carrying the ALK wild-type gene, ALK F1174L mutant gene and / or ALK F1196M gene and / or EML4-ALK mutant gene and / or NPM-ALK mutant gene, and can be used in the first-line treatment of anaplastic lymphoma kinase (ALK) positive late-stage non-small cell Lung cancer.
Owner:PRECEDO PHARMA CO LTD

Specific T cell receptor aiming at EGFR L858R gene mutation and application thereof

The invention provides a specific TCR for EGFR L858R gene mutation and an application of the specific TCR. The TCR has a property of binding to a neoantigen peptide KITDFGRAK-HLA-A*1101 complex derived from EGFR L858R gene mutation, and the TCR comprises a variable region and a constant region of an alpha chain and a beta chain. The T cell modified by the TCR has a specific killing effect on an HLA-A*1101 tumor cell mutated by an EGFR L858R gene. In addition, the invention also provides a pharmaceutical composition for treating tumors related to expression of the gene mutation, and the pharmaceutical composition has the characteristics of strong specificity and good individualized treatment effect.
Owner:天津亨佳生物科技发展有限公司

2, 6-disubstituted purine compound as well as preparation method and application thereof

The invention belongs to the technical field of anti-tumor pharmacy, and particularly relates to a 2, 6-disubstituted purine compound and application thereof, and the compound or pharmaceutically acceptable salt thereof, which can be used as an inhibitor of targeted EGFR L858R / T790M, has anti-tumor activity, can effectively inhibit the growth of cancer cells, and is simple in production method and low in cost.
Owner:ZIGONG NO 4 PEOPLES HOSPITAL

Specific T cell receptor (TCR) directed at EGFR L858R gene mutation and application thereof

The invention provides a specific TCR directed at EGFR L858R gene mutation and application of the specific TCR. The TCR has a property of binding to a neoantigen peptide KITDFGRAK-HLA-A*1101 complex derived from the EGFR L858R gene mutation, and the TCR comprises variable regions and constant regions of an alpha chain and a beta chain. A T cell modified by the TCR has a specific killing effect onan HLA-A*1101 tumor cell of EGFR L858R gene mutation. In addition, the invention also provides a pharmaceutical composition for treating tumors related to the expression of the gene mutation. The pharmaceutical composition has the characteristics of strong specificity and good individualized treatment effect.
Owner:天津亨佳生物科技发展有限公司

Specific T cell receptor (TCR) directed at EGFR L858R gene mutation and application thereof

The invention provides a specific TCR directed at EGFR L858R gene mutation and application thereof. The specific TCR has a property of binding to a neoantigen peptide KITDFGRAK-HLA-A*1101 complex derived from the EGFR L858R gene mutation, and the TCR comprises variable regions and constant regions of an alpha chain and a beta chain. A T cell modified by the TCR has a specific killing effect on anHLA-A*1101 tumor cell of EGFR L858R gene mutation. In addition, the invention also provides a pharmaceutical composition for treating tumors related to expression of the gene mutation. The pharmaceutical composition has the characteristics of strong specificity and good individualized treatment effect.
Owner:天津亨佳生物科技发展有限公司

Specific T cell receptor (TCR) specific to EGFR L858R gene mutation and application of TCR

The invention provides a specific T cell receptor (TCR) specific to EGFR L858R gene mutation and application of the TCR. The TCR has the characteristic of combining a new antigen peptide KITDFGRAK-HLA-A*1101 compound derived from the EGFR L858R gene mutation, and comprises variable regions and constant regions of an alpha chain and a beta chain. T cells modified by the TCR has specific killing effect on HLA-A*1101 tumor cells with the EGFR L858R gene mutation. In addition, the invention further provides a drug combination for treating tumors involved in expressing the gene mutation; the characteristics of strong specificity and good individualized treatment effect are realized.
Owner:天津亨佳生物科技发展有限公司

2-arylamine-4-amido quinazoline compound as well as preparation method and application thereof

The invention provides a 2-arylamine-4-amido quinazoline compound and a preparation method thereof. The 2-arylamine-4-amido quinazoline compound is shown in a formula I. The compound in the formula I has good inhibitory activity on EGFR L858R / T790M / C797S kinase and can be used for preparing drugs for treating diseases or symptoms related to tumors.
Owner:JIANGSU CHIA TAI FENGHAI PHARMA

EGFR L858R neoantigen epitope peptide, and application thereof to tumor treatment

The invention provides an EGFR L858R neoantigen epitope peptide, and application thereof to tumor treatment. The sequence of the EGFR L858R neoantigen epitope peptide comprises one or two different amino acid sequences shown as SEQ ID No.1 and SEQ ID No.2, or comprises an amino acid sequence formed by adding, deleting or replacing one or more amino acids to or from one or two different amino acidsequences shown as SEQ ID No.1 and SEQ ID No.2. The EGFR L858R neoantigen epitope peptide can activate T lymphocytes and specifically kill HLA-A*1101 tumor cells with EGFR L858R mutation, and is applied to preparation of cancer drugs for treating cancer such as non-small cell lung cancer.
Owner:天津亨佳生物科技发展有限公司

Inhibitor of t790m mutant egfr and its application in the preparation of antitumor drugs

The invention provides a T790M mutant EGFR inhibitor and an application of the same in preparation of antitumor drugs. The inhibitor is a pyrimidine compound with structural characteristics of a formula (I). The compound can inhibit various tumor cells and particularly can act on EGFR L858R / T790M and EGFR E 745_A750 / T790M lung cancer cells selectively, and the IC 50 of the compound is 10 timers and even 100 times higher than that of wild cancer cells. The compound is the protease inhibitor which is capable of overcoming prior EGFR-TKI drug resistance and has selectivity and can be applied to preparation of antitumor drugs. (img file= 'DDAS0000708455660000011. TIF' wi= '716' he= '576' / ).
Owner:PHARMA SHANGHAI

4-amide substituted pyrimidine targeted DDR1 inhibitor as well as preparation and anti-tumor activity application thereof

The invention discloses an N-substituted (5-chloro-6-(substituted phenoxy)pyrimidin-4-yl)benzamide targeted DDR1 small molecule inhibitor as well as preparation and application thereof. Provided is the N-substituted (5-chloro-6-(substituted phenoxy)pyrimidin-4-yl)benzamide target DDR1 inhibitor. The synthesized 45 compounds have low toxicity to normal lung cells, the best compound is C1 in 10 [mu]M DDR1 kinase preliminary screening, and the inhibition rate of the compound on DDR1 under 10 [mu] M is significantly superior to that of a lead compound YFQ07. In the aspect of cell experiments, theIC50 value of C1 to PC-9GR is significantly superior to that of YFQ07. In addition, it is found that the inhibition rate of the designed and synthesized compound on PC-9 cells (EGFR L858R mutation) mostly exceeds 50%, and the C1 can inhibit formation of lung cancer cell colonies and migration of tumor cells.
Owner:WENZHOU MEDICAL UNIV

Specific TCR pointing to EGFR L858R gene mutation and application of specific TCR

The invention provides a specific TCR pointing to EGFR L858R gene mutation and application of the specific TCR. The TCR has the characteristic of bonding to a new antigen peptide KITDFGRAK-HLA-A1101 complex derived from the EGFR L858R gene mutation and comprises variable and constant regions of alpha and beta chains. T cells modified with the TCR have a specific killing action on tumor cells HLA-A1101 expressing EGFR L858R gene mutation. In addition, the invention further provides a pharmaceutical composition for treating tumors involving expression of gene mutation. The pharmaceutical composition has the characteristics of high specificity and good individualized treatment effect.
Owner:天津亨佳生物科技发展有限公司

New application of ibrutinib

The invention discloses a new application of ibrutinib (PCI-32765). In particular, it is found that the ibrutinib can treat non-small cell lung cancer carrying EGFR T790M mutation and / or EGFR L858R mutation and / or EDFR delE746-A750 mutation, and especially, can treat the non-small cell lung cancer carrying double-mutation of EGFR L858R and EGFR T790M.
Owner:HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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