30 results about "Donor cornea" patented technology

The present disclosure relates to a corneal implant comprising a matrix material and a core part wherein the core part is arranged essentially in the middle of the matrix material. The core part is anti fouling in order to stop cell proliferation across said part. The present disclosure further relates to a method of preparing the said product and the use of the same.

A fixture and method is provided for in vitro storage of a cornea. The fixture includes a platform having a corneal-sceral rim receiving surface, a clamp having a mating surface for the cornel-scleral rim and handles. A locking mechanism is provided to secure a donor cornea between the clamp and platform. The combination cornea, platform and clamp are placed in a storage unit having a fluid preservation media. In one embodiment, the storage unit includes a vial having an optically clear closed end and an opened end. A lid is secured to the open end of the vial and engages the handles in order to stabilize the clamped cornea within the vial.

A donor corneal tissue cutting blade provided with integrally formed asymmetrical markers for facilitation identification of the anterior and posterior sections or portions of the donor corneal tissue is disclosed. The blade is a cylindrical body having, in one embodiment, a U-shaped extension which cooperates with the body to define a keyway. Protrusions may be formed on the body and / or extension to provide further markers.

The invention relates to a fluorescent quantitative PCR detection kit and a detection method of HBV and TP of donor corneas. The kit comprises a DNA (deoxyribonucleic acid) extraction reagent, a DNA amplification reagent and positive working standards, wherein the DNA amplification reagent comprises a premix reagent, a 20X fluorescent probe reinforcing agent, forward and reverse HBV primers, an HBV fluorescent probe, forward and reverse TP primers, a TP fluorescent probe, distilled water of ribose-free nuclease and HBV and TP negative control and positive control; the positive working standards are pUC57-HBV plasmids containing destination sequences amplified by the forward and reverse HBV primers and a pUC57-TP plasmids containing destination sequences amplified by the forward and reverse TP primers. The invention realizes the complete closed-tube detection on donor conjunctiva tissue specimens and does not need PCR aftertreatment, and thereby, cross contamination is avoided; meanwhile, real-time detection is adopted, and an obtained Ct value and the original template number are completely in linear correlation; the quantitative accuracy rate is extremely high, and the relative error is about 50 percent, and thereby, the invention is enough to adapt to the demands of clinical nucleic acid quantification.

A single-use adjustable depth trephine corneal punch device that allows a goal trephination depth of a donor cornea to be set and cut prior to a surgical procedure for cutting selected portions of an actual patient cornea. The corneal punch includes a punch block having a central well for receiving a donor cornea, a punch top mounted atop the punch block and having a central cylindrical opening coaxially aligned with the well, a punch blade mounted atop the punch top and through the opening cooperates with the top for combined limited rotation and limited axial movement from a reference or “start” point and into juxtaposed relation against the cornea and a limited axial downward movement during rotation to make a shallow cut in the cornea.

The invention discloses a method for screening the transparency of a film-shaped heterogeneous biological material, which comprises the steps of dehydration of the biological material sucrose, OCT embedding, layer-by-layer frozen sectioning, and light transmittance measurement with a spectrophotometer. Also provided is a highly transparent decellularized dermal matrix obtained by the above method. Through the present invention, the acellular dermal matrix with the best transparency can be effectively screened out, which can be used for lamellar keratoplasty, and the surgeon can use the above-mentioned matrix to replace the existing lamellar corneal stroma during the operation. At the same time, it can also effectively solve the contradiction of the current shortage of donor corneas.

The invention relates to an in vitro construction method of tissue engineered human corneal stroma. It is characterized in that the human corneal stromal cells are made into a cell suspension with special culture medium A for in vitro construction of tissue engineered human corneal stroma, and then the cell suspension is injected into the decellularized porcine corneal stroma carrier after rehydration with a special rehydration solution for carrier scaffolds Put the scaffold into a 24-well culture plate, add the special culture medium A for in vitro construction to culture in vitro, replace the special culture medium B for in vitro construction during the culture, and obtain the tissue-engineered human corneal stroma. The invention has low production cost and less time-consuming, and better maintains the integrity of the carrier bracket structure. The constructed tissue-engineered human corneal stroma is similar to the normal human corneal stroma in terms of morphology, structure and transparency, and has the characteristics of normal corneal stroma. Function, it can be used as a substitute for donated cornea for the repair of abnormal corneal stroma, so it can be used in mass production to meet the high-quality demand for tissue-engineered human corneal stroma.

Corneal transplant procedures may involve suturing an implant of healthy corneal tissue to a recipient cornea. The sutures may cause unwanted deformation of the corneal implant and the recipient cornea. A supporting structure may be embedded into the corneal implant to enhance the stability of the corneal implant and the recipient cornea and to reduce the likelihood of unwanted deformation when the corneal implant is sutured to the recipient cornea. According to one embodiment, a corneal implant includes donor corneal tissue extracted from a donor cornea. The donor corneal tissue includes an interior channel formed at a depth below an anterior surface. The corneal implant includes a supporting structure formed from non-tissue material and positioned in the channel.

The invention relates to a fluorescent quantitative PCR detection kit and a detection method of various viruses of donor corneas. The kit comprises an RNA (Ribonucleic Acid) extraction reagent, an RNA reverse transcription and amplification reagent and positive working standards, wherein the RNA reverse transcription (RT) and amplification reagent comprises a 2X RT-PCR premix reagent, polymerases, reverse transcriptases, forward and reverse HCV (hepatitis C virus) primers, an HIV1 (human immunodeficiency virus 1) primer, an RV (rubella virus) primer, HCV, HIV1 and RV fluorescent probes and HCV, HIV1 and RV negative and positive control; the positive working standards are pUC57-HCV plasmids containing destination sequences amplified by the HCV forward and positive primers and pUC57-HIV1 plasmids containing destination sequences amplified by the HIV1 forward and positive primers respectively. The invention realizes the complete closed-tube detection on donor conjunctiva tissue specimensand does not need PCR aftertreatment, and thereby, cross contamination is avoided; meanwhile, real-time detection is adopted and an obtained Ct value and the original template number are completely in linear correlation; the quantitative accuracy rate is extremely high, and the relative error is about 50 percent, and thereby, the invention is enough to adapt to the demands of clinical nucleic acid quantification.