The invention relates to an ultrasonic-guided visualized positioning device for an animal in-vivo
blood vessel. The ultrasonic-guided visualized positioning device comprises an ultrasonic diagnostic apparatus, an ultrasonic probe, a puncture guide frame, a
laser positioning device and a puncture needle, wherein the ultrasonic probe is electrically connected with the ultrasonic diagnostic apparatus, and the puncture guide frame is mounted at a working end close to the ultrasonic probe; the
laser positioning device comprises a supporting frame, the top end of the supporting frame is sequentiallyconnected with a
laser, a first locating ring and a second locating ring from left to right, horizontal-penetrative collimating locating holes are separately formed in dead centers of the first and second locating rings, and a laser
position line emitted from the laser penetrates through the collimating locating holes and a shot hole; the puncture needle is mounted at the bottom end of the supporting frame, the front end, close to a spine, of the puncture needle is mounted in a locating groove of the puncture guide frame, and the laser
position line is parallel to the axis of the puncture needle. According to the device, an
angle of incidence of a shot can be accurately determined, and the accurate positioning of a wound to an
experimental animal caused by the shot is ensured.