41 results about "Adenylic deaminase activity" patented technology

The present invention relates to a naturally occurring low molecular weight adenosine A3 receptor agonist (LMW-A3RAg) which is preferably obtained from a vertebrate tissue or a vertebrate-derived cell by extraction in a liquid medium. The LMW-A3RAg of the invention is characterized by the following feature: (i) it is obtainable from animal-derived tissue or cells; (ii) it filters through a filter with a maximal molecular weight cut-off of about 3,000 Daltons; (iii) it is water soluble, heat stable, non-proteinaceous and resistant to adenosine deaminase activity. The invention also concerns pharmaceutical compositions comprising the naturally occurring LMW-A3RAg of the invention and therapeutic methods comprising administering to a subject in need an effective amount of the naturally occurring A3RAg for achieving a therapeutic effect, the therapeutic effect comprises inhibition of adenylate cyclase in target cells.

Heinz(1980) makes use of the adenosine ammonia-deleted enzyme to change adenosine into hypo-yellow and gains H2O2 through the reaction of the enzyme1 and enzyme2. With the effect of the catalase and aldehyde hydrogen-deleted enzyme, Heinz measures the velocity of increase of the degree of NADPH absorbing light at 334 nms to gain adenosine ammonia-deleted enzyme's activity. The method's weakness is high cost of the reagents. The invention introduces Trinder's reaction to combine aniline type hydrogen and 4- to from a coloured product with the effect of H2O2 gained by the reaction of enzyme3 in the situation of the catalase. By watching the quantity of this coloured product, people can determine the adenosine ammonia-deleted enzyme's activity. The invention simplifies the Heinz's reaction system and lowers the cost. The invention uses the aniline compound ADOS, ADPS, ALPS, TODB, TOOS and TOPS as hydrogen provider for Trinder's reaction and increases the reaction's agility. The invention also involves a reagent box used for implementing the method mentioned above.

The invention discloses an adenosine deaminase diagnosing agent box through enzyme circulating augmentation method, density agent composition and component in the medical detecting and testing technical domain, which comprises the following parts: buffer, adenosine, alpha-ketoglutaric acid, glutamate dehydrogenase, glutamic oxidase, reduction type coenzyme and stabilizer, wherein the samples are blended according to certain bulk rate to do series of enzymatic reaction and places the reactants under ultraviolet / visible analyzer, which detects the absorbance dropping speed of major wavelength at 340nm to calculate the activity density of the adenosine deaminase. The invention improves the sensitivity and precision, which is convenient to spread and apply.

The invention relates to a method for detecting adenosine deaminase activity through liquid chromatography tandem mass spectrometry and screening an inhibitor thereof. The method comprises the following steps: reacting a sample (whole blood, serum or hemocyte) to be detected and substrate adenosine, or reacting the sample (traditional Chinese medicine or compound) to be detected, an adenosine deaminase and substrate adenosine; then detecting inosine content in the stop solution by the liquid chromatography tandem mass spectrometry; and calculating the activity of adenosine deaminase. The method has the advantages of being high in accuracy, high in precision, high in sensitivity, high in stability, simple to operate, low in cost, and high in throughput; an effective method is provided to clinically detect the adenosine deaminase activity and diagnose leukemia, typhoid fever, systemic lupus erythematosus, diabetes, hepatopathy and tumor, and the support is provided to screen the inhibitor of the adenosine deaminase in order to treat the abovementioned diseases.

The present invention relates to one kind of diagnostic adenosine deaminase reagent kit and method of measuring activity of adenosine deaminase, and belongs to the field of medical detection technology. The reagent kit of the present invention includes buffering liquid, 2-ketoglutarate, reduced coenzyme, glutamate dehydrogenase and adenosine. The sample is mixed with the reagent in certain volume ratio to produce reduced enzyme-linked reaction, and the reaction product is set under visible light analyzer to detect the absorbance descending speed or main wavelength or subsidiary wavelength, so as to obtain the activity of adenosine deaminase via calculation. The present invention obtains the determination results in visible light analyzer, and has high sensitivity, high precision and no contamination of endogenous matter.

The invention is to determine the activity of the Adenosine deaminase and the reagent box of it. The box includes buffer, adenosine, phosphate, nucleoside phosphorylase, xanthine oxidase, ethanol, peroxidase, oxidized coenzyme, aldehyde dehydrogenase and stabilizer. According to the proper ratio to mix the reagents, so the reagent can react and we can get the activity of the enzyme by detecting the absorbance of the main wave.

The invention relates to an adenosine deaminase diagnostic reagent (kit) which utilizes an enzyme colorimetric method and an enzyme linking immunoassay technology, and also relates to an adenosine deaminase activity concentration measuring method as well as composition and components of the reagent, belonging to the technical field of medical test measurement. The reagent (kit) mainly comprises the following components: buffer solution, coenzyme, adenosine, inosine nucleosidase, ribose 1-dehydrogenase and stabilizing agent; a sample is mixed with the reagent according to a certain volume proportion, so that a series of enzymatic reaction is carried out; and reactant is arranged under an ultraviolet / visible light analysis instrument, and the absorbance rising speed is detected when the main wavelength is 340nm, so that the activity concentration of the adenosine deaminase can be measured and calculated.

The invention relates to a diagnostic reagent (kit) of adenosine deaminase, which utilizes enzyme colorimetry and an enzyme linkage method technology. Meanwhile, the invention also relates to a method for measuring the activity concentration of the adenosine deaminase and the formation and the components of the reagent, which belong to the technical fields of the inspection and the measurement of medicine. The reagent (kit) comprises the main components of a buffer solution, reduced coenzyme, adenosine, oxyacid, amino acid dehydrogenase and a stabilizing agent; and a sample and the reagent generate a series of enzymatic reactions by mixing the sample and the reagent according to a certain volume ratio, then a reactant is put under an ultraviolet / visible light analyzer, and the descending speed of the absorbency of a position with the dominant wavelength of 340nm is detected, thereby measuring and calculating the magnitude of the activity concentration of the adenosine deaminase.

The invention relates to a method for determining the activity of adenosine deaminase, and also the reagent kit for adenosine deaminase diagnosis. The reagent kit comprises cushioning solution, adenosine, glutacid, deacidized type coenzyme, adenosine triphosphate, pyroracemic acid, phosphoenolpyruvate phosphatase, glutamine synthetase, pyruvic oxidase, phosphoenolpyruvate pyruvate carboxylase, malic dehydrogenase, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the activity of the adenosine deaminase can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention relates to an adenosine deaminase diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining adenosine deaminase activity concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, coenzyme, adenosine, adenosine triphosphate, inosine kinase, inosine monophosphate dehydrogenase and a stabilizer. The method for determining adenosine deaminase activity concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the activity concentration of adenosine deaminase.

The invention relates to a method for determining the activity of adenosine deaminase, and also the reagent kit for adenosine deaminase diagnosis. The reagent kit comprises cushioning solution, adenosine, inosine monophosphate, oxidized type coenzyme, guanosine monophosphate reductase, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the activity of the adenosine deaminase can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention relates to an adenosine deaminase diagnostic reagent (kit) which utilizes an enzyme colorimetric method and an enzyme linking immunoassay technology, and also relates to an adenosine deaminase activity concentration measuring method as well as composition and components of the reagent, belonging to the technical field of medical test measurement. The reagent (kit) mainly comprises the following components: buffer solution, coenzyme, adenosine, inosine nucleosidase, xanthine dehydrogenase and stabilizing agent; a sample is mixed with the reagent according to a certain volume proportion, so that a series of enzymatic reaction is carried out; and reactant is arranged under an ultraviolet / visible light analysis instrument, and the absorbance rising speed is detected when the main wavelength is 340nm, so that the activity concentration of the adenosine deaminase can be measured and calculated.

The invention relates to an adenosine deaminase diagnostic reagent (kit) which utilizes an enzyme colorimetric method and an enzyme linking immunoassay technology, and also relates to an adenosine deaminase activity concentration measuring method as well as composition and components of the reagent, belonging to the technical field of medical test measurement. The reagent (kit) mainly comprises the following components: buffer solution, coenzyme, adenosine, sodium bicarbonate (carbon dioxide), adenosine triphosphoric acid, glyceraldehyde-3-phosphoric acid, amine formyl phosphate synthase, glyceraldehyde-3-phosphoric acid dehydrogenase and stabilizing agent; a sample is mixed with the reagent according to a certain volume proportion, so that a series of enzymatic reaction is carried out; and reactant is arranged under an ultraviolet / visible light analysis instrument, and the absorbance rising speed is detected when the main wavelength is 340nm, so that the activity concentration of the adenosine deaminase can be measured and calculated.

The invention relates to an adenosine deaminase diagnostic kit utilizing technologies of an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring activity concentration of adenosine deaminase and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, coenzyme, adenosine, pyruvic acid, hydrogen peroxide, glycine oxidase, alanine dehydrogenase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the activity concentration of the adenosine deaminase.

The invention relates to a method for determining the activity of adenosine deaminase, and also the reagent kit for adenosine deaminase diagnosis. The reagent kit comprises cushioning solution, adenosine, adenosine triphosphate, glutacid, pyroracemic acid, ethanol, oxidized type coenzyme, glutamine synthetase, pyruvic oxidase, hydrogen peroxidase, aldehyde dehydrogenase, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the activity of the adenosine deaminase can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention relates to a method for determining the activity of adenosine deaminase, and also the reagent kit for adenosine deaminase diagnosis. The reagent kit comprises cushioning solution, adenosine, adenosine triphosphate, glutacid, pyroracemic acid, glutamine synthetase, pyruvic oxidase, peroxydase, deacidized chromogen combination, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the activity of the adenosine deaminase can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention is to determine the activity of the Adenosine deaminase and the reagent box of it. The box includes buffer, adenosine, phosphate, nucleoside phosphorylase, xanthine oxidase, peroxidase, urease, reduced chromogen and stabilizer. According to the proper ratio to mix the reagents, so the reagent can react and we can get the activity of the enzyme by detecting the absorbance of the main wave.

The invention relates to an adenosine deaminase diagnostic kit utilizing technologies of an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring activity concentration of adenosine deaminase and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, coenzyme, adenosine, phosphate group, ammonium chloride, 5'nucleotidase, ribonucleoside monophosphate reductase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the activity concentration of the adenosine deaminase.

The invention relates to a adenylate deaminase diagnostic reagent box utilizing the enzyme cycle amplification, the enzyme-colorimetric and the enzyme-linked techniques, meanwhile, the invention also relates to a method for detecting the activity consistence of the adenylate deaminase, and the compositions and the component of reagent, and belongs to the technical field of medical examination and determination. The reagent box provided by the invention contains buffer solution, reduced coenzyme, adenosine, pyruvic acid, alanine dehydrogenase, glycine oxidase and stabilizer. The sample is mixed with the reagent at certain volume ratio to impel the series of enzymatic reaction between the sample and the reagent, and the reactant is then put under an analyzer of visible light / ultraviolet ray to detect the descending speed of absorbency at the 340nm of the main wavelength, thus, the activity consistence of the adenylate deaminase can be calculated. The invention can completely obtain the needed test result with the help of the analyzer of visible light / ultraviolet ray.

The invention relates to a kit for diagnosing adenosine deaminase by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the active concentration of the adenosine deaminase, and belongs to the technology field of medical inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, adenosine, pyruvic acid, indole, tryptophanase, dopa decarboxylase, formate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the active concentration of the adenosine deaminase.

The invention relates to an adenosine deaminase diagnostic kit utilizing technologies of an enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring activity concentration of adenosine deaminase and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, coenzyme, adenosine, hydrogen peroxide, 2-oxoglutaric acid, D-glutamic oxidase, glutamate synthase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the activity concentration of the adenosine deaminase.

The invention relates to a kit for diagnosing adenosine deaminase by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the active concentration of the adenosine deaminase, and belongs to the technology field of medical inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, adenosine, pyruvic acid, indole, acetyl coenzyme A, tryptophanase, dopa decarboxylase, pyruvate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the active concentration of the adenosine deaminase.

The invention relates to a method for determining the activity of adenosine deaminase, further to an adenosine deaminase detection kit, and belongs to the technical field of medical test determination. According to the method, adenosine used as a substrate is converted and dehydrogenated by using purine nucleoside phosphorylase and xanthine dehydrogenase as tool enzymes, oxidized coenzyme is reduced to form a reduced coenzyme, and the enzyme reaction rate is calculated by detecting the generation amount of the reduced coenzyme I at a wavelength of 340 nm. The method of the present invention has advantages of less reaction steps, less kinds of raw materials, good reagent stability, wide linear range, high sensitivity, good accuracy and convenient promotion.

The invention relates to an adenosine deaminase diagnostic reagent (kit) which utilizes an enzyme colorimetric method and an enzyme linking immunoassay technology, and also relates to an adenosine deaminase activity concentration measuring method as well as composition and components of the reagent, belonging to the technical field of medical test measurement. The reagent (kit) mainly comprises the following components: buffer solution, coenzyme, adenosine, ferricytochrome C, calcium chloride, nitrite reductase, nitrate reductase and stabilizing agent; a sample is mixed with the reagent according to a certain volume proportion, so that a series of enzymatic reaction is carried out; and reactant is arranged under an ultraviolet / visible light analysis instrument, and the absorbance rising speed is detected when the main wavelength is 340nm, so that the activity concentration of the adenosine deaminase can be measured and calculated.

The invention relates to a kit for diagnosing adenosine deaminase by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the active concentration of the adenosine deaminase, and belongs to the technology field of medical inspection and measurement. The main components of the kit include a buffer solution, coenzyme, adenosine, nucleoside triphosphatase, xanthine oxidase, NAD(P)H oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the increase in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the active concentration of the adenosine deaminase.

The present invention relates to one kind of adenosine deaminase activity detecting method and the adenosine deaminase diagnosis reagent for the detection, and belongs to the field of medical detection technology. Adenosine, single-valent phosphate, nucleoside phosphorylase, xanthine oxidase, peroxidase and reductive chromoplast are combined to form the main component of the diagnosis reagent. Through serial enzyme linked reactions, the colorless reductive chromoplast is combination oxidized into color dye, so that dye content may be determined with visible light analyzer at 400-600 nm and adenosine deaminase activity may be detected for the diagnosis of several diseases. Compared with available technology, the created dyes have relatively high molar extinction coefficient and thus the present invention has high sensitivity and high precision. In addition, the present invention has no contamination of endogenous matter.

This invention relates to a method for measuring the active of the adenosine deamination enzyme and the digonose reagent box for adenosine deamination enzyme, which belongs to the physic checking measuring technique area. This reagent box includes cushion solution, adenosine, glutamic acid, 2-2-ketoglutaric acid,reducing assist enzyme,adenylic triphosphate acid, glutamine synthetic enzyme, glutamic acid synthetic enzyme, stabilizing agent, this method mixes the smple with the reagent by a certain volum proportion to make it fall into the enzyme couplet action, then puts the finally reactant under the biochemistry analyzer to check the absorbenfy changing situation of the main wave to work out the active of the adenosine deamination enzyme. This invention can achive the needed result completely through the biochemistry analyzer, the sensitivity and the definition is high, and it doesn't suffer the pollution from the environment.

The invention relates to a diagnostic reagent (kit) of adenosine deaminase, which utilizes enzyme colorimetry and an enzyme linkage method technology. Meanwhile, the invention also relates to a method for measuring the activity concentration of the adenosine deaminase and the composition and the components of the reagent, which belong to the technical fields of the inspection and the measurement of medicine. The reagent (kit) comprises the main components of a buffer solution, coenzyme, adenosine, oxidation type ferredoxin, ferredoxin nitrite reductase, nitrate reductase and a stabilizing agent; and a sample and the reagent generate a series of enzymatic reactions by mixing the sample and the reagent according to a certain volume ratio, then a reactant is put under an ultraviolet / visible light analyzer, and the ascending speed of the absorbency at the dominant wavelength of 340nm is detected, thereby measuring and calculating the activity concentration of the adenosine deaminase.

The invention relates to a kit for diagnosing adenosine deaminase by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the adenosine deaminase, and belongs to the technical field of medical inspection and measurement. The main components of the kit include a buffer solution, coenzyme, adenosine, 5'-nucleotidase, inosine monophosphate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the adenosine deaminase.

The present invention relates to one kind of adenosine deaminase activity detecting method and the adenosine deaminase diagnosis reagent for the detection, and belongs to the field of medical detection technology. Adenosine, single-valent phosphate, nucleoside phosphorylase, xanthine oxidase, peroxidase and reductive chromoplast are combined to form the main component of the diagnosis reagent. Through serial enzyme linked reactions, the colorless reductive chromoplast is combination oxidized into color dye, so that dye content may be determined with visible light analyzer at 400-600 nm and adenosine deaminase activity may be detected for the diagnosis of several diseases. Compared with available technology, the created dyes have relatively high molar extinction coefficient and thus the present invention has high sensitivity and high precision. In addition, the present invention has no contamination of endogenous matter.