30 results about "Adenosine kinase" patented technology

The invention belongs to the field of bioengineering, relates to preparation of ATP (adenosine triphosphoric acid), and provides a recombinant expression vector and a recombinant expression host containing the same. The recombinant expression vector contains polynucleotide of encoded adenosine kinase, adenylate kinase and acetokinase. The invention further provides a method for synthesizing ATP from the recombinant expression host. According to the invention, ATP is synthesized from the recombinant expression host by whole-cell catalysis, adenosine conversion rate, ATP yield and reaction efficiency are increased, and the reaction system contains simple components and is low in cost.

The present provides compounds capable of modulating IL-4 receptor-mediated IgE production, as well as IL-4 induced processes associated therewith, methods and kits for identifying such compounds that utilize an adenosine kinase as a surrogate analyte and methods of using the compounds in a variety of in vitro, in vitro and ex vivo contexts.

In the invention provides for a method of stimulating or increasing beta-cell replication or growth, by contacting a beta-cell with an inhibitor of adenosine kinase (ADK), an inhibitor of S-Adenosylhomocysteine hydrolase (SAHH) or an activator of AMP activated protein kinase (AMPK).

The present invention relates to an antibacterial composition containing, as an active ingredient, adenylate kinase or adenosine kinase (ADK) protein derived from Mycobacterium tuberculosis, and a composition for preventing or treating infectious diseases. In addition, the present invention relates to a composition for preventing or treating sepsis or septic shock. Furthermore, the present invention relates to a method for preventing, improving or treating an infectious disease comprising administering the present antibacterial composition. The ADK protein derived from Mycobacterium tuberculosis according to the present invention has excellent antibacterial activity selectively against gram-negative bacteria, and thus can be favorably used as an antibacterial composition against gram-negative bacteria or for the prevention or treatment of infectious diseases caused by gram-negative bacteria. In addition, the ADK protein has an excellent sepsis treatment effects, thus can be favorably used for the prevention or treatment of sepsis or septic shock.

The invention belongs to the field of bioengineering and relates to the preparation of adenosine triphosphate. The invention provides a recombinant expression vector and a recombinant expression host comprising the vector, the recombinant expression vector comprises polynucleotides encoding adenosine kinase, adenylate kinase and acetate kinase; the invention also provides the use of the A method for the synthesis of adenosine triphosphate by a recombinant expression host. The invention improves the adenosine conversion rate, ATP production rate and reaction efficiency by using the whole cell of the recombinant expression host to catalyze the synthesis of adenosine triphosphate, and meanwhile, the components of the reaction system are simple and the cost is low.

The invention relates to an enzyme of metabolic adenine nucleotide synthesized by cordyceps sinensis hirsutella sinensis from bailing producing bacteria to participate in adenosine starting, gene coding the enzyme and an application of the enzyme. The adenosine kinase amino acid sequence is shown as SEQ ID No (sequencer identifier number).1, and the coding gene correspondingly has the nucleotide sequence shown as SEQ ID No .2. The metabolic pathway of the adenosine synthesized adenine nucleotide is detailedly studied in the principle aspect, cloning DNA (deoxyribonucleic acid) containing the nucleotide sequence provided by the invention can be used for being transferred into engineering bacteria by transduction, conversion and combination methods, high expression of host adenine nucleotide is given through regulating the adenine nucleotide biosynthetic gene expression, an effective path is provided for the yield increase of the adenine nucleotide, and great application prospects are realized.

The present invention relates to a composition for preventing or treating sepsis or septic shock including a Mycobacterium tuberculosis-derived adenosine kinase (ADK) protein as an active ingredient. The Mycobacterium tuberculosis-derived ADK protein according to one exemplary embodiment of the present invention has an effect of inhibit binding between the LPS and cells by binding to LPS, and also has an excellent therapeutic effect against sepsis by enhancing viability in a sepsis animal model and suppressing inflammatory response in the lung and cell death in the spleen.

The present invention relates to the use of 4-anilinoquinazoline derivatives as adenosine-kinase inhibitors. The present invention also relates to a method for protecting tissues and organs like heart, brain and kidneys affected by ischemia, and for treating heart insufficiency, myocardium infarct, arrhythmia, arterial hypertension, atherosclerosis, coronary artery restenosis after angioplasty, chronic renal insufficiency, cerebral vascular accident, and chronic inflanunatory diseases (e.g., rheumatoid arthritis). The present invention also relates to the compound 6,7-dimethoxy-4-(3′-N′,N′-dimethylaminoanilino)quinazoline, or a pharmaceutically acceptable salt thereof, pharmaceutical composition comprising it and use of such compound in the manufacture of a medicament for treating or preventing diseases or conditions that are benefited from the adenosine-kinase inhibition.

The invention relates to an in vitro cell-free expression system incorporating a novel inorganic polyphosphate-based energy regeneration system. In certain embodiments, the invention includes a cell-free expression system where the cellular energy source, ATP, is regenerated from inorganic polyphosphate using a dual enzyme system. In this embodiment, this dual enzyme system may include thermostable Adenosyl Kinase, and / or Polyphosphate Kinase enzymes.

The invention discloses a method for improving the equilibrium conversion rate of D-psicose synthesized by taking D-glucose as a substrate through combination of phosphonase and an ATP regeneration system. D-psicose is prepared by taking D-glucose as a raw material through recombinant escherichia coli construction, wet thallus preparation and whole-cell catalytic reaction in sequence. The recombinant escherichia coli is used for simultaneously expressing the glucokinase, the 6-phosphoglucose isomerase, the D-psicose-6-phospho-3 epimerase, the D-psicose-6-phosphate-phosphoesterase and the adenosine kinase, and the recombinant escherichia coli is used for simultaneously expressing the glucokinase, the 6-phosphoglucose isomerase, the D-psicose-6-phosphate-phosphoesterase and the adenosine kinase. Wherein the construction process of the recombinant escherichia coli comprises introduction of an ATP regeneration system. According to the method, phosphoenzyme and an ATP regeneration system are combined, so that the conversion rate of synthesizing D-psicose from D-glucose is increased, and the addition of exogenous ATP is reduced. The method has the characteristics of simple preparation process, high product conversion rate, greenness and economy, and can be used for industrial production.