The invention discloses a production process of a hardened and tempered high-strength Q500E super-thick steel plate. The production method comprises process steps of molten iron pre-treating, converter steelmaking, external refining, continuous casting (dynamic soft pressing and electromagnetic stirring), heating, rolling, pre-straightening, online quenching, offline quenching, tempering, finishing, performance examining and ultrasonic-wave flaw detecting. The steel comprises the chemical components in percentage by mass: not greater than 0.15% of C, 0.20%-0.40% of Si, 1.00%-1.30% of Mn, not greater than 0.015% of P, not greater than 0.003% of S, 0.03%-0.06% of AlT, not greater than 0.10% of Nb+Ti+V, not greater than 1.0% of Cu+Ni, 0.40%-0.70% of Cr, 0.30%-0.60% of Mo, not greater than 0.0025% of B, and the balance of Fe and inevitable impurities. CEV is controlled to be not greater than 0.58, and the CEV is calculated according to a formula of CEV=C+Mn / 6+(Cr+Mo+V) / 5+(Ni+Cu) / 15. The produced high-performance hardened and tempered high-strength Q500E super-thick steel plate which is 100mm-120mm thick satisfies needs of manufacturing heavy machinery of a mine, a port, and the like.