The invention discloses a printing bottom plate fixing device and a 3D printing workbench provided therewith. The printing bottom plate fixing device includes a base frame, a lifting platform, a first fastening mechanism and a second fastening mechanism, and the upper surface of the lifting platform is provided with a bottom plate In the installation area, the structure of the first fastening mechanism and the structure of the second fastening mechanism are left-right symmetrical, and the first fastening mechanism includes the first lever assembly, the first stopper, the first hook body, the second stopper and the third stopper block, the first lever assembly includes a first cross bar, a first hinge seat and a first elastic member. When the lifting platform rises upwards, the two hooks will move away from each other and move upwards. The printing base can be placed on the base installation area, and then the lifting platform is lowered, and the two hooks are close to each other and moving downwards, which completes the fixing of the printing base. , on the contrary, you can remove the printing bottom plate; it is simple and convenient to install or remove the printing bottom plate, saving time and effort.