A vacuum toilet collection system and operation method with a transfer box, including a toilet, the toilet is connected to a dirt box through a sewage pipe, and a vacuum exhaust pipe is arranged on the dirt box; the toilet is also installed with air and water through the connecting pipe The operating system, the air-water operating system is connected to the water tank through the main pipe; it also includes a control unit, which is connected with the air-water operating system, toilet, and dirt tank for electrical signals, and the control unit is also connected with a flushing button; Box, and with the cooperation of the air-water operating system and the dirt box, it can conveniently complete the low-noise feces collection work, with good work reliability, high work efficiency and fast response. The present invention can reduce the noise in the private room under the condition that the passenger goes to the toilet, avoids relatively large noise from affecting the rest of the passenger, and improves the travel satisfaction of the passenger. At the same time, the vacuum feces collection system is equipped with a transfer box, which can reduce the start-up frequency of the vacuum generator, reduce the consumption of compressed air, and reduce the power consumption of the train.