The invention discloses a lossless transfer device for droplets. The device comprises a power generation part and a clamping part, the power generation part comprises a movable friction material and at least two fixed friction materials, the clamping part comprises a supporting mechanism, a left dielectric wetting clamping plate and a right dielectric wetting clamping plate, the left dielectric wetting clamping plate and the right dielectric wetting clamping plate are installed on the supporting mechanism, the movable friction materials are connected with the left dielectric wetting clamping plate, and the at least two fixed friction materials are connected with the right dielectric wetting clamping plate. The invention also discloses a lossless droplet transfer method and a droplet micro-reaction method. The device is more portable; by using a crank connecting rod mechanism, the left dielectric wetting clamping plate moves in the horizontal direction, and the device can adapt to liquid drops of different sizes conveniently; the accuracy of horizontal movement of the left clamping plate is ensured; a bolt and a spring are arranged, so that the left dielectric wetting clamping platecan horizontally move leftwards or rightwards conveniently; and the movement of droplets can be controlled, and the driving and fusion of the droplets are realized.