51 results about "Nitrate transporters" patented technology

The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I HDZip hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a NRT protein or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted YEP16; or having modulated expression of a Group I shaggy-like kinase or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.

Methods and compositions that affect yield and other agronomic characteristics in plants are disclosed. Methods of transgenic modulation and marker-assisted breeding by expressing NRT1.1 B are also disclosed, thereby improving the nitrogen utilization and grain yield in rice and other crops.

The present invention relates to a system for functional expression of higher plant nitrate transporter (Nrt) genes in Pichia pastoris, an in vivo nitrate uptake assay using these Pichia pastoris transformants, and an assay for readily identifying successful transformants.

The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I HDZip hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a NRT protein or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted YEP16; or having modulated expression of a Group I shaggy-like kinase or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.

The invention discloses application of rice OsMADS27 gene and protein in improving the nitrogen fertilizer use efficiency of rice. According to the application, it is found according to research thatthe rice OsMADS27 protein can interact with nitrate translocator cofactors OsNAR2.1, overexpression OsMADS27 gene can significantly promote the expression of nitrate translocator, thereby promoting the transfer and absorption of the rice to nitrate, and improving the nitrogen fertilizer use efficiency. The application of the rice OsMADS27 gene and protein in improving the nitrogen fertilizer use efficiency of the rice is helpful for researching key regulatory genes related to the nitrogen absorption of the rice, is of great significance for screening and breeding of nutrient efficient plant varieties, can also provide a theoretical basis and technical support for explaining a physiological mechanism of nitrogen utilization of the rice, improving the nitrogen utilization efficiency of the rice and genetic improvement of rice varieties, and has important theoretical significance and potential application value.

The invention discloses a tea tree NRT1 gene, a sequence of which is shown as SEQ ID No.1. The invention also discloses a tea tree NRT1 protein, a sequence of which is shown as SEQ ID No.2. The invention also discloses a gene expression method of the tea tree NRT1. A total length of the tea tree NRT1 gene sequence is 1880bp, 594 amino acids are encoded, the protein molecular weight is 65.92kD, and a theoretic isoelectric point is 9.07043. 12 transmembrane helical regions are contained, wherein a large hydrophilic ring exists between a sixth transmembrane helical region and a seventh transmembrane helical region. The protein is a hydrophilic protein, and the tea tree NRT1 is a constitutive gene. The mechanism for absorbing and assimilating NO3<-> in different tissue parts of a tea tree is shown to be possibly different, and the NRT1 is not only used as a nitrate transfer protein, but also is a signal acceptor sensing the nitrate.

The present invention relates to a system for functional expression of higher plant nitrate transporter (Nrt) genes in Pichia pastoris, an in vivo nitrate uptake assay using these Pichia pastoris transformants and an assay for readily identifying successful transformants.

The present invention provides a method for changing nitrogen utilization efficiency in a plant comprises regulating the expression of Arabidopsis NRT 1.7 or an orthologue thereof so that the nitrate remobilization from older leaves to young leaves in the plant is regulated, thereby the nitrogen utilization efficiency is changed. The present invention also provides a transgenic plant obtainable by transforming a plant with an expression construct with a high or low level of expression of NRT 1.7. On the other hand, the present invention yet provides a chimera nitrate transporter, a DNA molecule coding for this chimera transporter and an expression vector thereof.

The invention relates to transgenic plants with improved growth and nitrogen use efficiency expressing nitrate transporter gene, methods of making such plants and methods for improving growth and nitrogen use efficiency.

The invention relates to the technical field of plant genetic engineering and provides a transcription factor ZmNLP4 derived from corn and application thereof. A nucleotide sequence of the transcription factor is shown as SEQ ID NO.1, and a coded amino acid sequence of the transcription factor is shown as SEQ ID NO.2. Compared with a receptor Arabidopsis, the Arabidopsis in which the transcriptionfactor is transferred has the advantages that the total biomass is obviously increased, the content of in-vivo amino acids and total nitrogen is increased, the main root length and quantity of lateral roots are obviously increased, the yield per plant is obviously increased, and the expressions of nitrate transporters, nitrate reductase genes and nitrite reductase genes are obviously increased.