Nitrate transporter gene AtNRT1.1, coding protein and applications

Abstract

The invention provides a nitrate transporter gene AtNRT1.1, a coding protein and applications, and belongs to the plant molecular biology and transgenosis related technology fields. Through insertion of arabidopsis AtNRT1.1 gene T-DNA into a homozygous strain of a mutant salk_138710, functions of the AtNRT1.1 gene at aspects of plant low-nitrogen tolerance and efficient utilization of nitrogen are researched. Experiment results show that, a AtNRT1.1 gene knockout mutant has greener leaf color than wild type and slightly longer roots and shows a low nitrogen tolerance phenotype without any addition of exogenous nitrogen element. Under treatment condition of else addition of 0.1 nM and 5 mM of ammonium nitrate, the nitrogen content of the mutant root is higher than that of the wide type but the nitrogen content of the overground part is obviously lower than that of the wide type (P is equal to 0.0001), which shows the AtNRT1.1 gene has important regulation and control effects on nitrogen element absorption and transportation under different concentrations, and predicts that the silencing of the gene can raise plant low-nitrogen tolerance ability and has great economic value in plant nitrogen nutrition efficient molecular breeding.

Description

Technical field [0001] The invention belongs to the related technical field of plant molecular biology and transgene. Specifically, the response and action mechanism of the key gene of the Arabidopsis nitrate transporters (Nitrate transporters, NRTs) family, AtNRT1.1, under low nitrogen stress, and the application of this gene in the cultivation of new varieties of nutrient-efficient plants. Background technique [0002] Nitrogen is one of the essential nutrients for plants, and is a component of proteins, nucleic acids, chlorophyll, enzymes, etc. in plants. Insufficient nitrogen supply will affect the growth and development of plants and limit the yield of crops; while excessive nitrogen fertilizer application will reduce nitrogen use efficiency, which not only causes waste of resources, but also causes environmental pollution. One of the important ways to solve this problem is to tap the genetic potential of plants, clone low-nitrogen tolerance and high-efficiency nitroge...

AI Technical Summary

Problems solved by technology

But so far there is no report on the growth and development of the above-ground parts and nitrogen transport of this gene under different nitrogen concentrations.

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