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Hongyan strawberry nitrate transporter gene FaNRT2.7 and application thereof

A technology for nitrate transport and red-faced strawberries, applied in application, genetic engineering, plant genetic improvement, etc., to achieve high application value, promote maturity, and increase nitrate content

Pending Publication Date: 2022-05-31
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of how to elucidate the molecular mechanism of NRT transporter involved in the regulation of strawberry nitrogen metabolism, improve the nitrogen utilization rate in the strawberry production process and realize the cultivation of fertilizer-saving technology, and provide the red-faced strawberry nitrate transporter gene FaNRT2 .7 and its application

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  • Hongyan strawberry nitrate transporter gene FaNRT2.7 and application thereof
  • Hongyan strawberry nitrate transporter gene FaNRT2.7 and application thereof
  • Hongyan strawberry nitrate transporter gene FaNRT2.7 and application thereof

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Embodiment Construction

[0023] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.

[0024] 1. Cloning and analysis of the gene and promoter of the nitrate transporter gene FaNRTF2.7 in red strawberry.

[0025] The NRT2.7 gene was amplified using the prepared cDNA of strawberry fruit as a template and N27F and N27R as primers, and relevant bioinformatics analysis and phylogenetic tree analysis were carried out. Bioinformatics analysis using software or website found that: FaNRT2.7 contains a complete open reading frame of 1416bp, encoding 471 amino acids; the isoelectric point of the encoded amino acid sequence is 8.46, and the protein molecular weight is 50848.21; predicted transmembrane helices (TMHs) is 12, and the predicted amino acid number (AAs) of the transmembrane helix is ​​254.50042; there is no signal peptide in the amino acid sequence; the FaNRT2.7 protein domain is predicted a...

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Abstract

The invention relates to the technical field of plant genes, in particular to a Hongyan strawberry nitrate transporter gene FaNRT2.7 and application thereof, and provides cloning and analysis of the gene and a promoter of the Hongyan strawberry nitrate transporter gene FaNRT2.7 and application of analysis on response modes of different nitrogen forms, nitrate nitrogen, hormones and other abiotic stresses. Therefore, the method can be applied to screening germplasm materials, cultivating high-quality varieties and coping with abiotic stress according to different response states.

Description

technical field [0001] The invention relates to the technical field of plant genes, in particular to the red-faced strawberry nitrate transporter gene FaNRT2.7 and its application. Background technique [0002] Hongyan Strawberry (Fragaria x ananassaDuch.'Benihoppe') is a new variety in Japan. It has strong plant growth, large leaves, many new stems and branches, and the plant is erect and easy to cultivate and manage. Hongyan Strawberry, also known as Red Cheek, is an early-maturing cultivar bred by crossing Zhangji and Xingxiang in Shizuoka Prefecture, Japan. [0003] NRT2.7 is a seed-specific expression of high-affinity NO 3 - transport protein. Arabidopsis NRT2.7 is particularly expressed in seeds, especially at the late stage of seed maturation. It is located on the vacuolar membrane of reproductive organs and is responsible for the NO 3 - Transport from cytosol to vacuole to regulate NO in seeds 3 - content and dormancy. According to the research of Qian Yu et ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/08A01H6/74
CPCC07K14/415C12N15/8243
Inventor 郝福玲葛相君王琴孙启涵吴沐谦
Owner ANHUI AGRICULTURAL UNIVERSITY
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