Hongyan strawberry nitrate transporter gene FaNRT2.7 and application thereof
A technology for nitrate transport and red-faced strawberries, applied in application, genetic engineering, plant genetic improvement, etc., to achieve high application value, promote maturity, and increase nitrate content
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[0023] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.
[0024] 1. Cloning and analysis of the gene and promoter of the nitrate transporter gene FaNRTF2.7 in red strawberry.
[0025] The NRT2.7 gene was amplified using the prepared cDNA of strawberry fruit as a template and N27F and N27R as primers, and relevant bioinformatics analysis and phylogenetic tree analysis were carried out. Bioinformatics analysis using software or website found that: FaNRT2.7 contains a complete open reading frame of 1416bp, encoding 471 amino acids; the isoelectric point of the encoded amino acid sequence is 8.46, and the protein molecular weight is 50848.21; predicted transmembrane helices (TMHs) is 12, and the predicted amino acid number (AAs) of the transmembrane helix is 254.50042; there is no signal peptide in the amino acid sequence; the FaNRT2.7 protein domain is predicted a...
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