Primer group and kit for detecting rice nitrate transport protein gene, and detection method and application thereof

Abstract

The invention provides a primer group and kit for detecting a rice nitrate transport protein gene NRT1.1B, and a detection method and an application thereof, and relates to the technical field of ricebreeding. The primer group includes forward primers, reverse primers and general primers. Specific SNP loci for detecting the rice nitrate transport protein gene NRT1.1B can be screened by the primergroup, and the identification accuracy and detection efficiency of nitrogen-efficient germplasm resources are significantly improved; the detection method is accurate, reliable, and easy to operate,reduces the scale and frequency of determination of the nitrogen utilization rate, reduces the research and development costs, shortens breeding process and improves breeding efficiency. In the fieldof molecular assisted breeding, a technical support is provided for the creation of the nitrogen-efficient germplasm resources and cultivation of new nitrogen-efficient rice varieties.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows scientists to quickly determine if there's any mutations that affect plant growth or nutrients absorption by plants. It uses primers specifically designed to target certain genes called nrt1b (nucleotiphilin) and another protein called ubiquitous P450 monooxygenase-23a (UPO2) involved in photosynthesis pathways. These techniques help predict how crops will grow better based on their ability to absorb fertile substances from water sources like rainwater. By comparing these results against known data sets provided through experiments, we found that this approach could lead us to more precise identifications about root nodules containing important crop traits like nitrates. Overall, our inventors developed an improved technique for studying nitrotropic compounds related to rice roots.

Problems solved by technology

This patents discuss how different ways were used during rice growing to achieve better yields while maintaining stable supply overcomes issues such as excessively applied nitrogeietosynthesis agents like sulfur fertilizers leading to poor soil health and productivity rates. Current methods involve adding these chemical substances through various means including irrigations, managing weeds, controlling rootstock development, reducing nitrosion rates, promoting tolerance against disease attacks, enhancing grain weight, creating larger seed bases, and optimally combining multiple types of plants together within certain gains called multiples.

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