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Methods for improving rice grain yield

A technology for yield, rice, in the field of genetically altered plants and preparation of such plants, capable of addressing yield loss, low nitrogen availability, etc.

Pending Publication Date: 2022-01-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field, rice can be affected by many adverse conditions, which can lead to a huge loss in yield
One of the main limiting factors affecting yield is low nitrogen availability

Method used

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  • Methods for improving rice grain yield
  • Methods for improving rice grain yield
  • Methods for improving rice grain yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0246] Materials and methods

[0247] Plant material and growing conditions

[0248] OsNRT2.3TIILLING lines, backcross lines and transgenic lines (in this study) were surface sterilized with 30% (v / v) NaClO for 30 min and then rinsed thoroughly with water. Seedlings were grown in a climate chamber with 16 hours of light (30°C) and 8 hours of darkness (26°C). Plants used for field trials were grown at Nanjing Agricultural University in Nanjing, Jiangsu. The chemical properties of the experimental field soils were as described by Chen et al. (Chen et al., 2016)

[0249] Identification of the OsNRT2.3 TILLING line

[0250] When the seedlings were grown in IRRI for 3 weeks, DNA samples were collected and stored at −80 °C. DNA samples were extracted by CTAB method. We used two pairs of primers pOsNRT2.3-F / R and OsNRT2.3-F / R to amplify the promoter and gene of OsNRT2.3; each DNA sample was repeated 4 times. Product sequence by company name Sure. The sequence of the amplifi...

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Abstract

The present invention relates to methods for increasing rice grain yield and nitrogen use efficiency by increasing the expression of a nitrate transporter gene, as well as transgenic plants expressing increased expression of the gene and methods of making such plants.

Description

technical field [0001] The present invention relates to methods of increasing rice grain yield and nitrogen use efficiency by altering the splicing of nitrate transporter genes, as well as genetically altered plants having increased yield and methods of making such plants. Background technique [0002] In natural environments, plant growth and development are adversely affected by biotic and abiotic stresses. Environmental change is also an important factor affecting the normal growth of crops. As a result, plants have developed a number of unique mechanisms to cope with environmental fluctuations. [0003] Rice is one of the most important staple foods in the world, consumed by more than 50% of the world's population, especially in Asia (FAO, 2015). It is also one of the most important food crops nutritionally. However, in the field, rice can be affected by many adverse conditions, which can lead to a huge loss in yield. One of the main limiting factors affecting yield ...

Claims

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Application Information

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IPC IPC(8): C12N15/82
CPCC12N15/8261C07K14/415C12N15/8216Y02A40/146
Inventor 范晓荣
Owner NANJING AGRICULTURAL UNIVERSITY
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