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Use of SLC17A5 gene and coded protein thereof in preparing medicines for treating or diagnosing nitrate transfer or metabolic block or disorder related diseases

1. SLC17A5, nitrate transport technology, applied in metabolic diseases, gene therapy, drug combination, etc.

Inactive Publication Date: 2014-01-29
王松灵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Human and other mammalian nitrate transport channels have not been reported

Method used

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  • Use of SLC17A5 gene and coded protein thereof in preparing medicines for treating or diagnosing nitrate transfer or metabolic block or disorder related diseases
  • Use of SLC17A5 gene and coded protein thereof in preparing medicines for treating or diagnosing nitrate transfer or metabolic block or disorder related diseases
  • Use of SLC17A5 gene and coded protein thereof in preparing medicines for treating or diagnosing nitrate transfer or metabolic block or disorder related diseases

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0029] Experimental example 1 "Gastrointestinal - salivary gland circulation" interference research experiment

[0030] The experimental results showed that in patients with salivary gland hypofunction (Sjogren's syndrome, SS), nitrate and nitrite in saliva were significantly reduced, but significantly increased in urine. The concentration of nitrate in the saliva of healthy volunteers was 990.1±46.5μM and the concentration of nitrite was 122.2±17.6μM; the concentration of nitrate in the saliva of SS patients was 227.6±17.6μM and the concentration of nitrite was 79.9±38.8μM. The nitrate concentration in the urine of healthy volunteers was 1584.2±653.4 μM, and that of SS patients was 4603.9±2188.1 μM. The parotid gland atrophy miniature pig experiment also showed that the parotid gland atrophy led to a significant decrease in nitrate and nitrite in the saliva of the miniature pig, and a significant increase in the urine.

[0031] The experimental results suggest that salivary ...

experiment example 2

[0032] Experimental Example 2 Blocking Salivary Gland Nitrate Secretion Leads to Decreased Gastric Mucosa Protection

[0033] 1. Experimental materials and methods

[0034] 1) Ligation of parotid and submandibular gland ducts to block salivary gland nitrate secretion

[0035] 230-250g male SD rats were anesthetized with 10% chloral hydrate to incise the skin, and ligate bilateral parotid and submandibular gland ducts (P&SDL). Operation control group (sham): the skin was incised without ligation of the catheter.

[0036] 2) Rat stress test

[0037] Water immersion restraint stress (water-immersion-restraint, WIR) experiment was used. SD male rats were fasted for 18-20 hours, and after 1 hour of water deprivation, they were restrained in a rat holder, immersed vertically in water at 20±2°C, from the horizontal plane to the level of the xiphoid process, and lasted for 4 hours.

[0038] 3) Collect rat gastric juice to detect nitrate and nitrite

[0039] Pyloric ligation was p...

experiment example 3

[0048] Experimental example 3 Sialin localization and expression experiment

[0049] 1. Experimental materials and experimental methods

[0050] Human parotid gland and submandibular gland specimens were taken, washed with normal saline, and fixed with 4% paraformaldehyde (dissolved in 0.1M PBS solution) for 24 hours. Dehydration, fixation, embedding. Sections (4 μm thick) were deparaffinized, hydrated, and antigens were repaired by citrate microwaves. Routine immunohistochemical process, the expression and localization of proteins were detected by immunological double-labeling method.

[0051] 1.1 Colocalization experiment of Sialin and lysosomal membrane marker protein

[0052]The primary anti-Sialin antibody is: goat anti-human Sialin IgG antibody (Santa Cruz, CA, USA), with a dilution factor of 1:100. The primary antibody against LAMP-1 (Lysosomal-associated membrane protein1) was: mouse anti-human LAMP-1 IgG antibody (Santa Cruz, CA, USA), and the dilution factor was ...

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Abstract

The invention discloses use of an SLC17A5 gene and a coded protein thereof in preparing medicines for treating or diagnosing nitrate transfer or metabolic block or disorder related diseases. According to the use disclosed by the invention, a mass of experiments finally confirm that the SLC17A5 gene coded protein (Sialin protein) is a mammal nitrate transfer protein, and the SLC17A5 gene or the coded protein thereof can be used for preparing medicines for treating in vivo nitrate transfer and metabolic block related diseases as well as anti-hypertension and antishock medicines and medicines for treating myocardial infarction and preventing gastric ulcer and the like.

Description

technical field [0001] The present invention relates to the medical use of the SLC17A5 gene and its encoded protein, in particular to the use of the SLC17A5 gene and its encoded protein in the preparation of medicines for treating diseases related to nitrate transport or metabolic disorders in the body, and belongs to the medical use of the SLC17A5 gene and its encoded protein field. Background technique [0002] Nitrate is an inorganic substance that exists widely in nature. Initially, nitrate in mammalian organisms was regarded as a metabolite of nitric oxide, which was not biologically active. Later, people gradually realized that acidified nitrate has a bactericidal effect and can participate in host defense, so there have been new changes in the understanding of nitrate. Studies in recent years have shown that nitrate also has the functions of regulating blood pressure, regulating blood vessel tone, acting as nerve signal and immune regulation, and has potential thera...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K38/17A61P3/00A61P9/12A61P9/02A61P9/10A61P1/04A61P1/02A61P1/00A61P31/00C12Q1/68
Inventor 王松灵秦力铮刘细保邓大君英杜·阿伯德卡
Owner 王松灵
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