Nitrate transporter gene AtNRT1.5, and coding protein and application thereof

Abstract

The invention belongs to the field of plant molecular biology, and specifically relates to functional verification of arabidopsis thaliana nitrate transporter gene AtNRT1.5. A function of the gene in efficient utilization of plant nitrogen is studied by inserting T-DNA of the AtNRT1.5 gene into a homozygous line of a mutant CS870797. Experimental results show that the growth of the mutant on a high-nitrogen (MS) medium is inhibited; the root system of the mutant is obviously shorter than that of the wild type; and in a low nitrogen condition, the growths of the root systems of the mutant and the wild type have no difference, but dry weight of the mutant is lower than that of the wild type. Determination results of nitrogen content show that the nitrogen content in the root system of the mutant is higher than that of the wild type while the nitrogen content in the shoot is significantly lower than that of the wild type; and the difference is more obvious on the high nitrogen (MS) medium. The results demonstrate that after mutation of the ATNRT1.5 gene, nitrogen uptake capacity of the root system is not affected significantly while transportation capacity of the nitrogen from roots to shoots is reduced significantly. Over-expression of the gene is expected to improve the nitrogen utilization capability of plants and has relatively large economic value in plant nitrogen nutrition efficient molecular breeding.

Description

Technical field [0001] The invention belongs to the related technical field of plant molecular biology and transgene. Specifically, the response and mechanism of the key gene ATNRT1.5 of the Arabidopsis nitrate transporters (Nitrate transporters, NRTs) family under low nitrogen stress, as well as the application of this gene in the cultivation of new varieties of nutritionally efficient plants. Background technique [0002] Nitrogen is one of the essential nutrients for plants, and is a component of proteins, nucleic acids, chlorophyll, enzymes, etc. in plants. Insufficient nitrogen supply will affect the growth and development of plants and limit the yield of crops; while excessive nitrogen fertilizer application will reduce nitrogen use efficiency, which not only causes waste of resources, but also causes environmental pollution. One of the important ways to solve this problem is to tap the genetic potential of plants, clone low-nitrogen tolerance and high-efficiency nitr...

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Problems solved by technology

But so far there is no report that this gene regulates plant growth and development under different nitrogen conditions.

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