41 results about "Laryngeal squamous cell carcinoma" patented technology

The invention discloses a Hsa-miR-145-5p kit and a use of a mature body analogy of the Hsa-miR-145-5p. The Hsa-miR-145-5p kit comprises the following quantitative detection components of a Hsa-miR-145-5p gene: a Hsa-miR-145-5p PCR primer, dNTP with the concentration of 10mM, an RNase inhibitor, a microRNA / cDNA reverse transcription primer, a buffer solution with the concentration of 5 times, M-MLV, a SYBRGreenPCRMasterMix mixing liquid with the concentration of 2 times, deionized water, a Hsa-miR-145-5pfluorescence in-situ hybridization probe and a FSCN1 immunohistochemical component, wherein the Hsa-miR-145-5pfluorescence in-situ hybridization probe is a base sequence complemented with a Hsa-miR-145-5p basic group and is modified by DNA skeleton and fluorophore cy3. The Hsa-miR-145-5p kit can be used for molecular targeting treatment medicine for Laryngeal carcinoma.

The invention discloses a laryngeal squamous cell carcinoma cartilage invasion model. The laryngeal squamous cell carcinoma cartilage invasion model comprises a laryngeal squamous cell carcinoma invasion in-vitro experimental model, a laryngeal squamous cell carcinoma cartilage invasion semi-vitro experimental model, and a laryngeal squamous cell carcinoma cartilage invasion in-vivo experimental model. A method comprises the following steps of: closing the cup bottom of a Transwell chamber by using a polycarbonate microporous filter membrane; artificially reconstructing a basement membrane Matrigel and coating on the filter membrane; putting the Transwell chamber into a culture hole after the Matrigel is in a gel state, irradiating for 2h by using ultraviolet rays, and sterilizing for later use; adding an RPMI1640 culture medium containing 10 percent calf serum into the lower chamber, uniformly adding Hep-2 cells of laryngeal squamous cell carcinoma into the upper chamber, culturing, softly wiping away the Matrigel gel and cells which are not infiltrated at the cup bottom, immobilizing by using ethanol, and dyeing by using crystal violet; and counting the number of member-penetrating cells and taking an average value, wherein the relative number of the member-penetrating cells represents the invasiveness. In the invention, the laryngeal squamous cell carcinoma cartilage invasion model can be effectively established; and the discussion of an invasion mechanism of laryngeal squamous cell carcinoma on cartilages plays an important role in preventing the metastasis and relapse of laryngeal squamous cell carcinoma and selecting effective personalized therapeutic schedules.

The present invention discloses the application of miR-1207 and its target gene in detecting laryngeal squamous cell carcinoma, and the target gene is LOC339685 or LOC400706. The invention discloses the application of a reagent for detecting LOC339685 or LOC400706 in preparing a product for diagnosing laryngeal squamous cell carcinoma, and also discloses the application of LOC339685 or LOC400706 in preparing a drug for treating laryngeal squamous cell carcinoma.