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Laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 as well as detection method and application thereof

A molecular marker, a technology for laryngeal squamous cell carcinoma, which is applied in biochemical equipment and methods, microbial determination/inspection, medical preparations containing active ingredients, etc. It is not strong enough to achieve the effect of inhibiting malignant proliferation, stable results and strong primer specificity.

Pending Publication Date: 2020-07-24
FIRST HOSPITAL OF SHANXI MEDICAL UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

siRNA is a highly specific and efficient gene blocking technology. With the continuous development of technology, it has gradually become a powerful tool in cell biology. However, if the design is not reasonable, it is easy to produce off-target effects
[0004] In order to detect or verify the existence and expression of circRNA, the design of primers for real-time fluorescence quantification is very important. If the primer design is unreasonable and the specificity is not strong, it will lead to inaccurate measurement results

Method used

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  • Laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 as well as detection method and application thereof
  • Laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 as well as detection method and application thereof
  • Laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 as well as detection method and application thereof

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Embodiment 1

[0038] A detection method and application of the molecular marker hsa_circ_0018169 and its expression in laryngeal squamous cell carcinoma.

[0039] A molecular marker for laryngeal squamous cell carcinoma, the marker is hsa_circ_0018169, the sequence of which is shown in SEQ ID NO:1.

[0040] The laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 can be applied in the detection and prognosis of laryngeal squamous cell carcinoma.

[0041] The laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 is used in the preparation of an auxiliary diagnostic kit for laryngeal squamous cell carcinoma.

[0042] A method for detecting molecular expression of laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 comprises designing real-time fluorescent quantitative primers for hsa_circ_0018169, and obtaining the expression of hsa_circ_0018169 by using a real-time fluorescent quantitative detection method. Including the following steps:

[0043...

Embodiment 2

[0077] The siRNA of hsa_circ_0018169 was synthesized by using the laryngeal cancer molecular marker hsa_circ_0018169 of the present invention.

[0078] First, siRNA for hsa_circ_0018169 was synthesized.

[0079] According to the siRNA design principle, the synthetic sequence contains the circularization site of the circular RNA, and the sequence with a fragment length of 18-25bp is used as the candidate target sequence. In principle, the siRNA with a GC content of about 40%-50% is compared with the database through the database. Human genome sequences were compared to ensure no homology. The designed siRNA sequence of the present invention is as follows:

[0080] Sense strand: 5'-CCUGUAGACAGGUAAAUAC-3'

[0081] Antisense strand: 5'-GUAUUUACCUGUCUACAGG-3'

[0082] Send the above siRNA sequence to Suzhou Gemma Company for chemical synthesis, and add two deoxyribonucleotides dTdT at the 3' end at the same time to increase the stability of siRNA and prevent degradation.

[008...

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Abstract

The invention discloses a laryngeal squamous cell carcinoma molecular marker hsa_circ-0018169 as well as a detection method and application thereof. The nucleotide sequence of the laryngeal squamous cell carcinoma molecular marker hsa_circ-0018169 is as shown in SEQ ID NO:1. The laryngeal squamous cell carcinoma molecular marker hsa_circ_0018169 is applied to detection and prognosis of laryngeal squamous cell carcinoma. The laryngeal squamous cell carcinoma molecular marker hsa_circ-0018169 is applied to the preparation of a laryngeal squamous cell carcinoma auxiliary diagnosis kit. The invention provides a sequence of siRNA (small interfering ribonucleic acid) of the hsa_circ-0018169. The siRNA of the hsa_circ-0018169 is used for knocking down the expression of the hsa_circ-0018169 in laryngeal squamous cell carcinoma cells, has the effect of inhibiting malignant proliferation of the laryngeal squamous cell carcinoma cells, and is used for preparing laryngeal squamous cell carcinoma drugs; a primer of the hsa_circ-0018169 is high in specificity and stable in result, and a basis is provided for the biological research of the hsa_circ-0018169; and the siRNA designed by the inventioncan effectively reduce the expression quantity of hsa_circ-0018169, and has the characteristics of high efficiency and quick action.

Description

technical field [0001] The invention relates to the technical field of cellular and molecular biology, in particular to a molecular marker hsa_circ_0018169 for laryngeal squamous cell carcinoma and its detection method and application. Background technique [0002] Head and neck cancer is one of the six major tumors in the world, and laryngeal squamous cell carcinoma is one of the most common head and neck cancers, mainly concentrated in northern regions including Shanxi. At present, the main treatment methods are surgery, radiotherapy and chemotherapy. Although the diagnosis and treatment of laryngeal squamous cell carcinoma have made great progress in recent years, the incidence rate is still on the rise, and the malignant proliferation caused by in situ recurrence of laryngeal squamous cell carcinoma is still one of the main adverse factors that seriously affect the improvement of its prognosis. Patient survival and quality of life are greatly affected. [0003] In rece...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113A61K48/00A61K31/713A61P35/00
CPCC12Q1/6886A61K31/713A61P35/00C12Q2600/118C12Q2600/178C12Q2600/158Y02A50/30
Inventor 吴勇延高伟郭慧娜郑希望张宇良代丰升代力李仲珣侯睿
Owner FIRST HOSPITAL OF SHANXI MEDICAL UNIV
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