Laryngeal squamous cell carcinoma cartilage invasion model

Abstract

The invention discloses a laryngeal squamous cell carcinoma cartilage invasion model. The laryngeal squamous cell carcinoma cartilage invasion model comprises a laryngeal squamous cell carcinoma invasion in-vitro experimental model, a laryngeal squamous cell carcinoma cartilage invasion semi-vitro experimental model, and a laryngeal squamous cell carcinoma cartilage invasion in-vivo experimental model. A method comprises the following steps of: closing the cup bottom of a Transwell chamber by using a polycarbonate microporous filter membrane; artificially reconstructing a basement membrane Matrigel and coating on the filter membrane; putting the Transwell chamber into a culture hole after the Matrigel is in a gel state, irradiating for 2h by using ultraviolet rays, and sterilizing for later use; adding an RPMI1640 culture medium containing 10 percent calf serum into the lower chamber, uniformly adding Hep-2 cells of laryngeal squamous cell carcinoma into the upper chamber, culturing, softly wiping away the Matrigel gel and cells which are not infiltrated at the cup bottom, immobilizing by using ethanol, and dyeing by using crystal violet; and counting the number of member-penetrating cells and taking an average value, wherein the relative number of the member-penetrating cells represents the invasiveness. In the invention, the laryngeal squamous cell carcinoma cartilage invasion model can be effectively established; and the discussion of an invasion mechanism of laryngeal squamous cell carcinoma on cartilages plays an important role in preventing the metastasis and relapse of laryngeal squamous cell carcinoma and selecting effective personalized therapeutic schedules.

Description

technical field [0001] The invention relates to a cartilage invasion model of laryngeal squamous cell carcinoma, belonging to the technical field of tumor invasion and metastasis in vitro experimental models. Background technique [0002] At present, laryngeal cancer is one of the common malignant tumors in the head and neck, which seriously endangers human health. Surrounded by a dense connective tissue membrane around the larynx, it is difficult for intravenous chemotherapy drugs to reach the lesion. After years of clinical observation, the curative effect of radiotherapy is not good. Therefore, surgical resection is still the main treatment for laryngeal cancer. However, due to the important physiological functions of the larynx, the postoperative breathing, swallowing and vocalization functions of some patients are seriously affected, which brings a lot of inconvenience to life. Nevertheless, some patients still died of recurrence and metastasis. The vast majority of l...

AI Technical Summary

Problems solved by technology

However, it is often found clinically that there is infiltration of the surrounding tissues, especially in cases involving the laryngeal cartilage, which are prone to metastasis and recurrence. Once the recurrence of laryngeal squamous cell carcinoma is very poor, the 5-year survival rate is often as low as 60%.

So far, there are no relevant reports on cartilage invasion models of laryngeal carcinoma

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