LOC100507599 as molecular marker for diagnosis of laryngeal squamous cell carcinoma
A technology for laryngeal squamous cell carcinoma and products, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems of high postoperative recurrence rate and difficulty in early diagnosis of laryngeal cancer.
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Embodiment 1
[0028] Example 1 Screening for differentially expressed molecules
[0029] 1. Research object
[0030] The cancer tissues and corresponding paracancerous tissues of 5 patients with laryngeal squamous cell carcinoma were collected. Inclusion and exclusion criteria: ①Patients have no primary tumor other than the primary tumor, and the primary tumor has not metastasized; ②No history of cardiovascular and cerebrovascular diseases such as diabetes and hypertension; ③No infectious diseases such as hepatitis, syphilis, tuberculosis, and HIV Medical history; ④ no family history of genetic diseases; ⑤ no radiotherapy, chemotherapy and biological therapy before operation.
[0031] Sampling precautions: When cutting tumor specimens, pay attention to cutting non-necrotic tumor tissue in the central area of the tumor. When cutting adjacent normal tissues, try to keep away from the tumor as far as possible, and be more than 1.5cm away from the edge of the tumor. Try to cut mucosal epithe...
Embodiment 2
[0086] Example 2 Large sample verification screened out differentially expressed molecules
[0087] 1. Sample collection
[0088] According to the method of Example 1, the cancer tissues and paracancerous tissues of 30 patients with laryngeal squamous cell carcinoma were collected.
[0089] 2. Validation at the transcript level
[0090] Reagents: Reverse transcription kit (DDR037A) was purchased from Treasure Bioengineering (Dalian) Co., Ltd. SYBR Premix Ex Taq for real-time quantitative PCR (polymerase chain reaction) TM (Tli RNaseHPlus) kit was produced by Japan Takara Company.
[0091] 2.1 Extraction of total tissue RNA
[0092] Step is with embodiment 1.
[0093] 2.2 Reverse transcription
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