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Non-coding RNA closely related to laryngeal squamous cell carcinoma

A long-chain non-coding technology for laryngeal squamous cell carcinoma, which is applied in the detection/testing of microorganisms, medical preparations containing active ingredients, biochemical equipment and methods, etc., can solve the high recurrence rate after surgery and the difficulty of early diagnosis of laryngeal cancer And other issues

Pending Publication Date: 2020-08-28
BEIJING MEDINTELL BIOMED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reasons are mainly manifested in three aspects: First, the early clinical manifestations of laryngeal cancer are diverse, which brings great difficulty to the early diagnosis of laryngeal cancer. Most patients fail to receive timely and effective treatment in the early stage. Molecular markers are the key to improving the early diagnosis rate of laryngeal cancer
Second, the treatment of laryngeal cancer is mainly based on surgery, supplemented by radiotherapy and chemotherapy. Although partial laryngectomy and the early stage of glottic laryngeal cancer are treated with C02 laser treatment, the laryngeal function is completely or partially preserved, and postoperative complications Therefore, the quality of life of patients is improved, but the postoperative recurrence rate is still high. If the tumor-promoting genes or tumor suppressor factors of laryngeal cancer can be found out, and potential drug targets can be screened out, it will be a great opportunity for the development of laryngeal cancer. Cancer-related specific vaccines or / and new anti-cancer drugs lay the foundation

Method used

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  • Non-coding RNA closely related to laryngeal squamous cell carcinoma
  • Non-coding RNA closely related to laryngeal squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Differential Expression Molecular Verification

[0045] 1. Research object

[0046] The cancer tissues and corresponding paracancerous tissues of 45 patients with laryngeal squamous cell carcinoma were collected. Inclusion and exclusion criteria: ①Patients have no primary tumor other than the primary tumor, and the primary tumor has not metastasized; ②No history of cardiovascular and cerebrovascular diseases such as diabetes and hypertension; ③No infectious diseases such as hepatitis, syphilis, tuberculosis, and HIV Medical history; ④ no family history of genetic diseases; ⑤ no radiotherapy, chemotherapy and biological therapy before operation.

[0047] Sampling precautions: When cutting tumor specimens, pay attention to cutting non-necrotic tumor tissue in the central area of ​​the tumor. When cutting normal adjacent tissues, try to stay away from the tumor as far as possible, and be more than 1.5cm away from the edge of the tumor. Try to cut mucosal epithel...

Embodiment 2

[0096] Example 2 LncRNA overexpression

[0097] 1. Cell culture

[0098] The human laryngeal squamous carcinoma cell line Hep2 was incubated in RPMI1640 medium containing 10% fetal bovine serum and 1% P / S at 37°C and 5% CO 2 , Cultivated in an incubator with a relative humidity of 90%. The medium was changed once every 2-3 days, and the cells grew well and showed a monolayer adherent growth. Routine digestion with 0.25% EDTA-containing trypsin was used for passage.

[0099] 2. Transfection

[0100] 1) Treatment of cells before transfection

[0101] The day before transfection, seed 3-5×10 on a 6-well culture plate 5 Cells / well were cultured in antibiotic-free medium for one day, and the cell density was 30-50% at the time of transfection, and replaced with serum-free medium before transfection.

[0102] 2) LncRNA overexpression

[0103]Find the full-length sequence of long-chain non-coding RNA in NCBI, and submit it to Shanghai Gemma Company to construct an overexpressi...

Embodiment 3

[0112] Example 3 The effect of LncRNA expression on the proliferation of laryngeal squamous cell carcinoma cells

[0113] WST-1 method was used to detect the effect of lncRNA expression on the proliferation of laryngeal squamous cell carcinoma cells.

[0114] On the second day after cell transfection, adjust the cell density to 1×10 2 / μl, inoculated in a 96-well plate (100 μl / well), with 3 replicate wells for each group. 37°C, 5% CO 2 After culturing for 72 hours, 10 μl of WST-1 was added and incubated for 2 hours, and the optical density (D) value of the cells was detected at a wavelength of 450 nm with a microplate reader.

[0115] The results are shown in Table 2, and the difference between the groups was statistically significant (P<0.05). It shows that promoting the expression of LOC285627, LOC100506860 and LOC388692 can inhibit the proliferation of laryngeal squamous cell carcinoma cells.

[0116] Table 2 Proliferation statistics of laryngeal squamous cell carcinoma...

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Abstract

The invention discloses a non-coding RNA closely related to laryngeal squamous cell carcinoma, and the non-coding RNA is LOC100506860. Experiments of the invention prove that the LOC100506860 is closely related to diagnosis and treatment of the laryngeal squamous cell carcinoma, so that the LOC100506860 can be used as a diagnosis and treatment marker to be applied clinically.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a non-coding RNA closely related to laryngeal squamous cell carcinoma. Background technique [0002] Laryngeal cancer is a common malignant tumor in the head and neck, accounting for the second place in head and neck tumors, and its incidence rate accounts for about 5% of systemic malignant tumors. Most squamous cell carcinomas originate in the vocal cords, accounting for about 60%. Huang Xinhui et al conducted a retrospective analysis on 306 cases of laryngeal malignant tumors and found that squamous cell carcinoma accounted for 99.7% of all malignant tumors, among which squamous cell carcinoma with high and moderate differentiation was the main type; the primary site of laryngeal cancer was mostly glottic type, Followed by supraglottic type, subglottic type; smoking, drinking are closely related to the occurrence of tumors. Although surgery, radiotherapy, and chemotherapy can alleviat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00A61P35/00C12Q2600/118C12Q2600/158C12Q2600/178C12Q2600/166C12Q2600/136
Inventor 杨承刚孙耀兰
Owner BEIJING MEDINTELL BIOMED CO LTD
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