A
system is provided for sensing an
analyte in a breath sample. The
system includes a breath bag, a
cartridge and a base. The breath bag contains the breath sample. The bag includes a
mouthpiece fixedly disposed on the breath bag. The
cartridge includes an interactant that reacts with the
analyte and generates a change in an optical characteristic relative to a reference. The base includes a flow path, a breath bag
receiver for detachably receiving and retaining the
mouthpiece of the breath bag in fluid communication with the flow path, and a
cartridge receiver that detachably receives and retains the cartridge in the base, such that the base engages the cartridge so that the interactant is in fluid communication with the flow path. The base further includes a flow
handling system in fluid communication with the flow path, an optical subsystem for sensing the change in the optical characteristic, a processor operatively coupled to the flow
handling system and the optical subsystem, and a
user interface operatively coupled to the processor and comprising a start command. Upon user selection of the start command, the processor is configured to automatically regulate the flow
handling system to move the breath sample in the flow path and to contact the breath sample and the interactant. Upon the occurrence of a predetermined process parameter, the processor is configured to automatically regulate the optical subsystem to sense the change in the optical characteristic, to correlate the sensing of the optical
system with information about the
analyte in the breath sample, and to output the information about the analyte in the breath sample to the
user interface. Related methods also are provided.