The invention belongs to the field of biotechnology and biomedicine, and particularly relates to a kit for detecting inversion mutation of intron 22 and intron 1 of a severe hemophilia A disease-causing gene. The severe hemophilia A disease-causing gene mutation detection kit comprises Inv22 PCR (polymerase chain reaction) liquid and Inv1 PCR liquid, the Inv22 PCR liquid comprises three primers for detecting gene inversion of the intron 22 of the severe hemophilia A disease-causing gene, and the Inv1 PCR liquid comprises three primers for detecting gene inversion of the intron 1 of the severe hemophilia A disease-causing gene. Long fragment amplification stability of Inv22, detection sensitivity, PCR product quantity and electrophoresis detection abundance are improved, the problems of short preservation time, poor temperature tolerance and the like of reagents are solved by further adding Taq DNA (deoxyribonucleic acid) polymerase inhibitors, and stability of clinical application of the kit is improved.