46 results about "Developmental genes" patented technology

The invention discloses a wheat leaf developmental gene TaWRKY76 and an application thereof. The nucleotide sequence of the gene complementary deoxyribonucleic acid (cDNA) is shown in SEQ ID No.1. The invention further discloses an application of the gene TaWRKY76 in culture of plants with curled leaves. Experiments prove that the wheat TaWRKY76 can be introduced into plant cells, so that the plants can obtain a phenotype of curled leaves, advantages are provided for improving photosynthesis, and the gene can be widely applied to culture of high-yield crops varieties.

Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.

The invention discloses a gene for controlling male reproduction and development of rice and application of the gene. The gene has a nucleotide sequence represented by SEQ ID NO.1, and a protein encoded by the gene has an amino acid sequence represented by SEQ ID NO.2. By targeting a LOC_Os08g20730 gene design target by virtue of a CRISPR / Cas9 gene editing system, a receptor is a middle flower 11, and a targeted T0 generation phenotype are shown in the figures 1, 2, 3 and 4 in the description; and sequencing analysis shows that anther development abnormality occurs in a homozygously mutated plant or a biallelically mutated plant and pollen does not exist. The analysis of a homozygously mutated T1 generation shows that the separation proportion of fertile plants to sterile plants is 3 to 1. The integration shows that the gene is capable of regulating and controlling the reproduction and development of rice, once the gene in the rice is damaged, the anther development abnormality occurs, and non-pollen sterility occurs. According to the obtained cell nucleus gene for controlling the reproduction and development of the rice, an intelligent sterile line can be created by virtue of a molecular measure, and excellent parents in the production are improved into excellent sterile lines, so that the heterosis utilization is expanded.

The invention relates to a method for isolating and regulating peanut embryo development genes, which belongs to the field of functional genomics. AhHsfa4A The gene sequence is shown as SEQ ID NO: 1, which will be screened to obtain the closely related important genes that are induced by calcium to cause programmed death of peanut early germ cells AhHsfa4A , and obtained from the constructed whole-tissue full-length library by improved RACE technology AhHsfa4A The full sequence of the gene. The present invention establishes a method (SSHaLL) for efficiently screening important genes of peanut early embryos induced by calcium deficiency, and uses this method to screen an important gene that causes programmed death of early peanut embryos AhHsfa4A And its expression was identified. The invention has laid a good foundation for efficient screening of important differentially expressed genes in peanut embryo development, thereby further clarifying the molecular mechanism of peanut embryo development.

PiggyBac transposases engineered to increase stability in a cell. The engineered piggyBac transposases are useful for stably transforming cells, cell line development, genome modification, and improving titer of recombinant proteins, among other uses.

The invention discloses a plant anther specific promoter and the application. The promoter is the oligodeoxynucleotide with the length of 179bp to 1174bp. The 3'end of the promoter at least contains 996 site to 1174 site nucleic acid sequence at 5' end of sequence 1 in the self-sequence table, which extends along the nucleic acid arrangement mode of the sequence 1. The anther specific promoter has important application prospect for the function analysis and discrimination of the growth and development gene of the plant anther, the construction of artificial sterile system, the prevention of plant transgenic drift or escape and the extension of shelf life of flowers etc.

The invention discloses a gene regulating the growth and development of rice coleoptile OsBEAR1 For its application, the MSU ID of the gene is "LOC_Os11g15210", encoding the amino acid sequence shown in SEQ ID NO:3. Knockout the gene mutation in Nipponbare by gene editing method, knockout OsBEAR1 The growth and development of the coleoptiles of the rice seedlings were accelerated and were significantly longer than those of the recipient rice seedlings. The growth of the coleoptile is beneficial to rice germination and field direct seeding, and the invention provides a basis for the transformation and cultivation of rice varieties suitable for field direct direct seeding.