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Tomato gene SLMBP21 and application thereof

A tomato and gene technology, applied in the field of genetic engineering, can solve problems such as unknown functions and different functions, and achieve the effect of good application value

Active Publication Date: 2014-03-26
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In Arabidopsis, the genes of this subfamily have redundant functions, but the three SEP genes reported in tomato have different functions, indicating that the function of the genes of this subfamily in tomato is similar to that in Arabidopsis It may be very different, and the functions of two other SEP-like genes in tomato, LeMADS1 and SLMBP21 are currently unknown

Method used

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  • Tomato gene SLMBP21 and application thereof
  • Tomato gene SLMBP21 and application thereof
  • Tomato gene SLMBP21 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Cloning of full-length tomato SLMBP21 gene

[0031] 1) Using a forward primer with a nucleotide sequence as shown in SEQ ID No.3 and a reverse primer with a nucleotide sequence as shown in SEQ ID No.4, clone SLMBP21 from the pedicel cDNA of tomato Zhongshu No. 4 The sequence of the coding region of the gene;

[0032] PCR program: 94°C, 5 minutes; 94°C, 30 seconds; 55°C, 30 seconds; 72°C, 45 seconds; repeat 35 times; 72°C, 10 minutes.

[0033] PCR system: 2×EasyTaq PCR SuperMix (Quanshijin Company) 25μl;

[0034] Forward primer (10μM) 2μl;

[0035] Reverse primer (10μM) 2μl;

[0036] DNA template 5μl;

[0037] Double distilled water to make up 50μl.

[0038]The above PCR product was directly cloned and connected to pEASY-T1Simple according to the TA cloning method (such as image 3 shown); the ligation product was transformed into Escherichia coli DH5α and multiplied in it, and the positive clone was sequenced and screened to obtain the sequence;

[0039...

Embodiment 2

[0057] Example 2 Sequence Structure Analysis of Tomato SLMBP21 Protein

[0058] Tomato SLMBP21 has the characteristic sequence region of MADS-box family proteins: MADS region (about the first 60 amino acids), which has 69% similarity with SEP4 protein sequence in Arabidopsis and 84% similarity with LeMADS1 protein sequence in tomato . The result is as figure 1 shown.

Embodiment 3

[0059] Example 3: Sequence structure analysis of tomato SLMBP21 protein

[0060] The results of phylogenetic analysis showed that SLMBP21 belonged to the SEP subfamily, and belonged to the same branch of the evolutionary tree as SEP1 / 2 / 3 / 4 in Arabidopsis and Ph FBP2 / 4 / 5 / 9 / 23 in Petunia. Other members of this subfamily in tomato include TM5, TM29, RIN and LeMADS1.

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Abstract

The invention provides a tomato protein SLMBP21 and a tomato gene SLMBP21 for coding the protein, wherein the nucleotide sequence of the gene is shown in SEQ ID No.1. The invention has the advantages that the tomato gene SLMBP21 belongs to a tomato abscission zone developmental gene in an MADS-box gene family, is capable of inhibiting the expression of the gene SLMBP21 so as to obviously influence the development of a tomato abscission zone to produce a transgenic plant with no tomato abscission zone or an incompletely developed abscission zone. The tomato protein SLMBP21 can be used for solving the problems of blossom drop and fruit drop of various crops, vegetables, fruits and flowers and the problem of industrial picking of tomatoes; moreover, different form the situation that the development of inflorescence in a JOINTLESS mutant is affected, the development of inflorescence in the SLMBP21transgenic plant is not affected, which means that the gene SLMBP21 has higher application value.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a gene related to the development of a tomato detached area and its application in the process of organ shedding. Background technique [0002] Organ shedding is an important process in the process of plant development. The shedding of leaves, the shedding of flowers and fruits, and the cracking of fruit pods all belong to the category of organ shedding. At the same time, organ shedding is also an important agronomic trait, which affects the yield and quality of many crops, such as the flower and fruit drop of fruit trees that we are familiar with, and the bud drop and boll drop of cotton. The part where organ shedding occurs is called the abscission zone or abscission zone, which is the layer of smaller cells distributed at the base of the petiole, flower stalk, or fruiting stalk. Before the organ is about to fall off, the cell wall and intercellular layer of these layers of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 毛龙刘旦梅李爱丽
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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